산화적 손상에 의해 유발된 심근세포 독성에 대한 補陽還五湯의 방어효과
Protective Effects of Boyanghwanoh-tang on Zinc-mediated Cytotoxicity in H9c2 Cardiomyoblast Cells
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Abstract
The water extract of Boyanghwanoh-tang has been used for treatment of ischemic vascular disease in oriental medicine. However, little is known about the mechanism by which the water extract of Boyanghwanoh-tang rescues cells from these damages. Therefore, this study was designed to evaluate the protective effects of Boyanghwanoh-tang on zinc-mediated cytotoxicity in H9c2 cardiomyoblast cells. This study demonstrates that, after treatment of H9c2 cells with zinc, there was a decrease in cell viability in a dose dependent manner, and there was a chromatin condensation. Zinc induced the change of cell morphology. In addition, zinc induced mitochondrial dysfunction. Zinc-induced H9c2 cell death was remarkably prevented by the pretreatment of Boyanghwanoh-tang consistently with increase of the peroxoredoxin 1, 2, 3, 5, and 6 expression. Taken together, the results suggest that zinc induced severe cell death in H9c2 cardiomyoblast cells, and that protective effects of Boyanghwanoh-tang against oxidative injuries are achieved through regulation of peroxiredoin expression.
참고문헌 (27)
- "國譯 醫林改錯", pp.112~ (1998), 圓光大學校 出版局
- "中藥 方劑 現代 硏究大典", pp.723~6 (1996), 科學出版社
- "Free radicals and myocardial reperfusion injury", 49, pp.545~5 (1993)
- "Free radicals as mediators of tissue injury and disease", 23, pp.21~48 (1993)
- "Mice with targeted mutation of peroxiredoxin 6 develop normally but are susceptible to oxidative stress", 278(27), pp.25179~90 (2003)
- "補陽還五湯今用", 10(2), pp.50~2 (1997)
- "血栓症 및 高粘度血症에 관한 補陽還五湯의 實驗的 硏究", pp.30~46 (1988)
- "談談補陽還五湯與腦血管意外", 8, pp.7~ (1984)
- "補陽還五湯煎湯液이 家 의 血壓降下에 미치는 영향" (1985), 원광대학교 대학원
- "補陽還五湯이 혈압 및 국소뇌혈류량에 미치는 영향", 15(5), pp.682~6 (2001)
- "보양환오탕이 영양혈청결핍에 의한 PC12세포의 고사에 미치는 영향" (2000), 원광대학교 대학원
- "補陽還五湯의 冠心病,心絞痛 治療 44例", 15(1), pp.25~ (1997)
- "補陽還五湯加味의 3度 房室傳導阻滯 治療", 19(4), pp.26~ (1996)
- "임상의학 오늘의 진단 및 치료", pp.401~32 (1999), 한우리
- "In free radicals in biology and medicine", pp.~9 (1989), Clarendon Press
- "A critical physiological role of zinc in the structure and function of biomembranes", 28(13), pp.1425~38 (1981)
- "Zinc metabolism in the brain:relevance to human neurodegenerative disorders", 4(3-4), pp.137~69 (1997)
- "Novel biphasic effect of pyrrolidine dithiocarbamate on neuronal cell viability is mediated by the differential regulation of intracellular zinc and copper ion levels", 59(1), pp.117~25 (2000)
- "Micromolar concentrations of Zn2+ antagonize NMDA and GABA responses of hippocampal neurons", 328(6131), pp.640~3 (1987)
- "Voltage-dependent block by zinc of single calcium channels in mouse myotubes", 425, pp.563~78 (1990)
- "Brief exposure to zinc is toxic to cortical neurons", 71(3), pp.351~5 (1986)
- "contribution of energy failure attributable to loss of NAD and inhibition of glycolysis", 20(9), pp.3139~46 (2000), Zinc-induced cortical neuronal death
- "Zinc accumulation in adriamycin-induced cardiomyopathy in rats effects of melatonin", (4), pp.204~10 (1999)
- "Zinc and brain injury", 21, pp.347~75 (1998)
- "최신임상정신의학", pp.138~ (1988), 하나의학사
- "Two enzymes in one two yeast peroxiredoxins display oxidative stress-dependent switching from a peroxidase to a molecular chaperone function", 117(5), pp.625~35 (2004)
- "regulates apoptotic signaling by mitochondria", 279(40), pp.41975~84 (2004)