Imperatorin을 처리한 HL-60 백혈병 세포주에서 대규모 유전자 분석 발현 연구
Studies on Gene Expression of Imperatorin treated in HL-60 cell line using High-throughput Gene Expression Analysis Techniques
0
조회
0
다운로드
0
인용
0
참고문헌
초록
Abstract
Imperatorin, a biologically active furanocoumarin from the roots of Angelica dahurica (Umbelliferae), was mutagenic and induced transformation of mouse fibroblast cell lines, whereas it provided inhibiting effects on mutagenesis and carcinogenesis induced by various carcinogens. Furthermore, it has been suggested that imperatorin may have potential anticarcinogenic effects when administered orally in the diet. In addition to its anticarcinogenic properties, imperatorin has been shown to possess anticancer activities. We investigated the macro scale gene expression analysis on the HL-60 cells treated with imperatorin. Imperatorin (10 μM) were used to treat the cells for 6h, 12h, 24h, 48h, and 72h. In a human cDNAchip study of 10,000 genes evaluated 6, 12, 24, 48, 72 hours after treated with imperatorin in HL-60 cells. Hierarchical cluster against the genes which showed expression changes by more than 2 fold. Three hundred eighty six genes were grouped into 6 clusters by a hierarchical clustering algorithm. Pathway analysis using gene microarray pathway profiler that is a computer application designed to visualize gene expression data on screen representing biological pathways and groupings of genes.
참고문헌 (16)
- "지형준 Angelica속 생약의 정유성분에 관한 연구", pp.121~125 (1990)
- "Oral administration of naturally occurring coumarins leads to altered phase I and II enzyme activities and reduced DNA adduct formation by polycyclic aromatic hydrocarbons in various tissues of SENCAR mice", carci(genesis), pp.73~82 (2001)
- "Antiproliferative effect of isopentenylated coumarins on several cancer cell lines", tomo ya(y m) (1905)
- "Effect of coumarins on HL-60 cell differentiation", tomo ya(y m), pp.2505~12 (2000)
- "a furanocoumarin from Angelica dahurica induces cytochrome c-dependent apoptosis in human promyelocytic leukaemia", pp.40~8 (2002)
- "Cytotoxic activity of coumarins from the fruits of Cnidium monnieri on leukemia cell lines", pp.1091~5 (2003)
- "Coumarins from Opopanax chironium New dihydrofuranocoumarins and differential induction of apoptosis by imperatorin and heraclenin", appendi(g), pp.532~6 (2004)
- "a novel apoptosis-related gene cloned from human leukemia cell line TF-1 could enhance apoptosis of some tumor cells induced by growth factor withdrawa", pp.203~210 (1999)
- "A polyadenylate binding protein localized to the granules of cytolytic lymphocytes induces DNA fragmentation in target cells", pp.629~639 (1991)
- "novel serine", threonine kinases related to death-associated protein kinase that trigger apoptosis, 1998, 29066~29071
- "Mutation of Chinese Hamster V79 cells and transformation and mutation of mouse fibroblast C3H", 10T1, pp.1983, 43(3)[DOI]
- "The mutagenicities of seven coumarin derivatives and a furan derivative isolated from three medicinal plants", pp.4 521~6 30 (1984)
- "Amplification of a gene encoding a p53-associated protein in human sarcomas", pp.80~358 83 (1992)
- "The DNA repair endonuclease XPG binds to proliferating cell nuclear antigen and shares sequence elements with the PCNA-binding regions of FEN-1and cyclin-dependent kinase inhibitor p21", pp.24522~24529 (1997)
- "Activation of cdc2 protein kinase during mitosis in human cells", pp.17~26 (1988)
- "Expression of c-fos oncogene during hepatocarcinogenesis liver regeneration and in synchronized HTC cells", pp.427~34 (1985)