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淸熱消毒飮이 HL-60 세포주의 Apoptosis에 미치는 영향

Study on the influence of Cheongyulsodokeum that effects on apoptosis of HL-60 tumor cell

최정화|김종한|박수연|배진석
대한한방안이비인후피부과학회 (The Korean Medicine Opthalmology&Otolaryngology&Dermatology Society)|2007.04|한방안이비인후피부과학회지 (The Journal of Korean Medicine Ophthalmology&Otorhinolaryngology&Dermatology)|제20권 제1호|pp. 66-79

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Abstract

Objectives: This study was carried out to evaluate anti-tummor effect about apoptosis of Cheongyulsodok-Eum (CSE) Results: 1. Anti-tumor(HL-60 cells) effects of CSE water extracts(Exts) were more effective in high density.(IC50::572 ㎍/㎖) 2. The generation of O2- in HL-60 cells were according to the concentration of CSE water Exts, specially more effective on 100 ㎍/㎖ and 1000 ㎍/㎖ concentration. 3. The SOD activities in HL-60 cells were in proportion as cytotoxicity against HL-60 cells of CSE water Exts. 4. The GPx activities in HL-60 cells were in proportion as cytotoxicity against HL-60 cells of CSE water Exts(more effective on 100 ㎍/㎖ and 1000 ㎍/㎖ concentration), but the catalase activities in HL-60 cells were not effective. 5. DPPH radical scavenging activity of CSE water Exts was effective.(3 ㎍/㎖:31.2±5.2 %, 10 ㎍/㎖: 49.6±7.3 %, 30 ㎍/㎖:35.8±5.7 % 100 ㎍/㎖:42.3±6.4 %) 6. The results of cytotoxicity against HL-60 cells of CSE were as follows. 1) In hexane fraction, the cytotoxicity against HL-60 cells(IC50:592 ㎍/㎖) was more effective than against NIH3T3 cells. 2) In ethyl acetate fraction, the cytotoxicity against HL-60 cells was not effective. 3) In butanol fraction, the cytotoxicity against HL-60 cells(IC50:306 ㎍/㎖) was more effective than against NIH3T3 cells. 4) In H2O fraction, the cytotoxicity against HL-60 cells was not effective. Conclusion: These result suggest that CSE has antioxidative effects and anti-tumor effects by apoptosis of free radical(O2-) activity, especially butanol and hexane fraction from water extract has more effective in anti-tumor effects.

참고문헌 (25)

  1. "암 동서의 결합치료 권1", pp.258~268 (1999), 익산:원광대학교출판국
  2. "Cancer undefeated", 336, pp.1569~1574 (1997)
  3. "Multidrug resistance modulators PSC 833 and CsA show diferential capacity to induce apoptosis in lymphoid leukemia cel lines independently of their MDR phenotype", 27(5), pp.413~423 (2003)
  4. "消積保中丸의 抗腫瘍效果에 대한 實驗的 硏究", 2(1), pp.43~56 (1996)
  5. "內托羌活湯이 腫瘍 및 免疫調節機能에 미치는 實驗的 硏究" (1996), 圓光大學校 大學院(博士)
  6. "內消升麻湯의 抗癌效果 硏究" (2006), 東新大學校 大學院(博士)
  7. "복분자와 당귀 열수추출물의 마우스를 이용한 항암 및 항스트레스 효과", 14(4), pp.206~211 (2006)
  8. "부평의 면역 및 항암 활성 연구", 19(3), pp.117~127 (2004)
  9. "淸熱消毒飮과 구성약재의 암세포 독성 항균 및 항산화 효과", 20(4), pp.41~51 (2005)
  10. "淸熱消毒飮이 실험적 염증 및 혈전증에 미치는 영향", 9(2), pp.267~293 (1995)
  11. "T h e activity of antioxidants and suppression of cancer cell proliferation in extracts of Orostachys japonicus A", 11(1), pp.31~39 (2003)
  12. "Cytotoxicity of Ethanol Extract of Raphanuse Sativus on a Human Lung Cancer Cel Line", 33(2), pp.287~290 (2004)
  13. "Cytotoxicity and Multidrug-Resistance Reversing Activity of Extracts from Gamma-Irradiated Coix lachryma-jobi L. Var. ma-yuen Stapf seed", 34(5), pp.613~618 (2005)
  14. "Dnizot Fd and Rita L. Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J. Imunol. Methods. 1986", pp.271~277
  15. "W. R. Oxidative stress hypothesis in Alzheimer disease. Free Radical Bio. Med. 1994", pp.134~139
  16. "I. Superoxide dismutase. An enzymatic function for erythrocuprein", pp.6049~6054
  17. "Comparative study of superoxide dismutase glutathione peroxidase levels in erythrocytes of different animals. Biochem. Biophys. Res. Commun. 1977", pp.1525~1531
  18. "A basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer 1972", pp.239~57
  19. "Autophatgy as a cell death and tumor supressor mechanism", 23, pp.2891~2906 (2004)
  20. "Role of Bcl-2 family proteins in a non-apoptotic programmed cell death dependent on autophagy genes", 6, pp.1221~1228 (2004)
  21. "Role of autophagy in temozolomide-induced cytotoxicity for malignant glioma cells", 11, pp.448~457 (2004)
  22. "I. Superoxide sensitivity of Escherichiacoli 6-phosphogluconate dehydratase. J. Biol. Chem. 1991", pp.1478~1483
  23. "Immediate effects of anticancer drugs on mitochondrial oxygen consumption", 66, pp.977~987 (2003)
  24. "Boyum A. Isolation of leukocytes from human blood. Scan. J. Clin. Invest. 1968", pp.9~15
  25. "Antioxidant effects and application as natural ingredients of Korean sanguisorbae officinalis L", 47, pp.244~250 (2004)

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