녹차 추출물 중 카테킨 성분 농도에 따른 항균활성 비교 연구
Studies on the Comparison of Antibacterial Activity by Catechin Concentration in Green Tea Extract
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Abstract
Catechin products in green tea extract was prepared to investigate antibacterial activity on the pathogenic bacteria. Survival of pathogenic bacteria (MASA - methicillin resistant Staphylococcus aureus, E.coli O157 and S. typhimurium Sal-13) in tryptic soy agar(TSA) containing Catechin products powder incubated at various concentration was evaluated. TSA containing 0~2%(w/v) of Catechin products was inoculated approximately 104 CFU/㎖ of pathogenic bacteria and incubated at 37℃ for 24 hours. The plate counting technique and clear zoon test were used to test survival effect of the Catechin products. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) was derived from the survival curves of pathogenic bacteria. S. typhimurium Sal-13 was the most sensitive strain to Catechin products. This result suggested that Catechin products can be used as an effective natural antibacterial agent.
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