聰明湯과 木槿皮聰明湯 열수추출물, 초미세분말제형이 microglia 및 기억력 감퇴 병태모델에 미치는 영향
Effects of ChongMyung-Tang and ChongMyung-Tang added Hibiscus syriacus Hot water extract & Ultra-fine Powder on Microglia and Memory Deficit Model
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Abstract
This experiment was designed to investigate the effect of the CMT and MCMT hot water extract & ultra-fine powder on microglia and memory deficit model. The effects of the CMT and MCMT hot water extract on expression of IL-1β, IL-6, TNF-α, NOS-Ⅱ, COX-2, IL-10, TGF-β1 mRNA and production of IL-1β, IL-6, TNF-α, NO, ROS in BV2 microglial cell line treated by lipopolysacchaide(LPS) ; serum glucose, uric acid, AChE activity of the memory deficit mice induced by scopolamine ; behavior of the memory deficit mice induced by scopolamine and were investigated, respectively. The CMT and MCMT hot water extract suppressed the expression of IL-1β, IL-6, TNF-α, NOS-Ⅱ, COX-2 mRNA, production of IL-1β, IL-6, TNF-α, NO, ROS and increased the expression of IL-10, TGF-β1 mRNA in BV2 microglial cell line treated by LPS. The MCMT hot water extract & ultra-fine powder increased glucose, decreased uric acid and AChE significantly in the serum of the memory deficit mice induced by scopolamine. The CMT and MCMT hot water extract & ultra-fine powder groups showed significantly inhibitory effect on the scopolamine-induced impairment of memory in the experiment of Morris water maze. According to the above result, it is suggested that the CMT and MCMT hot water extract & ultra-fine powder might be usefully applied for prevention and treatment of dementia.
참고문헌 (33)
- 대한한방신경정신과학회편, "한방신경정신의학", pp.311~316 (2005), 집문당
- 조추용, "치매 예방과 케어", pp.~49 (2002)
- Cacquevel, M.,, "Cytokines in neuroinflammation and Alzheimer's disease", pp.529~534 (2004)
- Cacquevel, M.,, "In vivo mapping of cerebral acetylcholinesterase activity in aging and Alzheimer's disease", mi(shima), pp.691~699 (1999)
- Mhatre, M.,, "Oxidative stress and neuroinflammation in Alzheimer's disease and amyotrophic lateral sclerosis Journal of Alzheimers disease", pp.147~157 (2004)
- 안준익, "Beta-amyloid의 분자생물학", pp.11~16 (2001)
- 張介賓, "張氏景岳全書", pp.610~611 (1978)
- 최병만, "木槿皮가 CT-105로 유도된 Alzheimer's Disease 병태 모델에 미치는 영향", pp.119~139 (2004)
- 박지운, "聰明湯과 木槿皮聰明湯이 CT105와 βA로 유도된 Alzheimer's Disease病態 모델에 미치는 영향" (2004)
- 최현정, "한약제형 선호도에 관한 설문조사", pp.356~367 (2004)
- Song, L.L.,, "Study on pharmacology of ultra-fine particles compound Rehmannia", pp.436~439 (2002)
- Skehan, "New colorimetric cytotoxicity assay for anticancer drug screening Journal of the National Cancer Institute", pp.1107~1112 (1990)
- Diez, M.,, "Neuropeptides in hippocampus and cortex in transgenic mice overexpressing V717F beta-amyloid precursor protein- initial observations", pp.259~286 (2000)
- Hunot, S.,, "FcepsilonRⅡ", CD23 is expressed in Parkinson's disease and induces production of nitric oxide and tumor necrosis factor-alpha in glial cells, 1999, pp.hu(t), 3440~3447
- Tuppo, E.,, "The role of inflammation in Alzheimer's disease The International Journal of Biochemistry & Cell Biology", pp.289~305 (2005)
- Zhiming Suo,, "Alzheimer's β-amyloid peptides induce inflammatory cascade in human vascular cells the roles of cytokines and CD40", pp.1 110~2 117 (1998)
- Peskind, E.R.,, "Cerebrospinal fluid S100B is elevated in the earlier stages of Alzheimer's disease", pp.5 409~6 413 (2001)
- 이귀녕, "임상병리파일", pp.169~173 (2000), 의학문화사
- Foster, N.L.,, "crossover study of tacrine and lecithin in Alzheimer's disease", pp.260~266 (1996)
- Sramek, J.J.,, "Review of the acetylcholinesterase inhibitor galanthamine", pp.2393~2402 (2000)
- Trabace, L.,, "Biochemical and neurobehavioral profile of CHF2819 orally active acetylcholinesterase inhibitor for Alzheimer's disease" (2000)
- Geerts H, "Indicators of neuroprotection with galantamine", 64(60), pp.519~524 (2005)
- Kang, J.,, "Reactive oxygen species mediate A beta(25-35)-induced activation of BV-2 microglia", pp.25 1449~35 1452 (2001)
- Chang, J.W.,, "Two-dimensional gel analysis of secreted proteins induced by interleukin-1beta in rat astrocytes", pp.5 349~6 359 (2001)
- 康秉秀 외, "surface corrugation is sufficient to improve aerosol performance of powders", pp.148~649 152 (2005)
- Lee, "Cytokine production by human fetal microglia and astrocytes Differential induction by lipopolysaccharide and IL-1 beta The Journal of Immunology", pp.2659~2667 (1993)
- Nick, J.A.,, "Activation of p38 mitogen-activated protein kinase in human neutrophils by lipopolysaccharide", j immu(l), pp.4867~4875 (1996)
- Marcheselli, V.L.,, "Sustained induction of prostaglandin endoperoxide-2 by seizure in hippocampus The Journal of biological chemistry", pp.24794~24799 (1997)
- Pasinatti, G.M.,, "Cyclooxygenase-2 expression is increased in frontal cortex of Alzheimer's disease brain", pp.319~324 (1998)
- Yen, G.C.,, "Nitric oxide-scavenging and antioxidant effects of Uraria crinita root", pp.471~478 (2001)
- Mates, J.M.,, "Antioxidant enzymes and human disease" (1999)
- Ebert, U.,, "Scopolamine model of dementia European Journal of Clinical Investigation", pp.944~949 (1998)
- Ebert, U.,, "Scopolamine model of dementia European Journal of Clinical Investigation", pp.944~949 (1998)