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산수유의 유리자유기에 의한 간손상 보호효과

Study on the Protective Effect of Corni Fructus against Free Radical Mediated Liver Damage

김준기|김철호|김동욱|하기태|김영미|최달영
한의병리학회 (The Society of Pathology in Korean Medicine)|2007.12|동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)|제21권 제6호|pp. 1415-1423

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참고문헌

주제분류한의학

초록

Abstract

Carbon tetrachloride (CCl4)-induced liver injury depends on a toxic agent that has to be metabolized by the liver NAPDH-cytochrome P450 enzyme system to a highly reactive intermediate. Alternations in the activity of cytochrome P450 enzymes affect the susceptibility to hepatic injury from CCl4. In this study, we evaluated the potential protective activity of the traditional Korean medicinal herb, Corni fructus (CF), against an experimental model of hepatotoxicity induced by CCl4. The CF exhibited a hepatoprotective activity against CCl4-induced liver damage in Sprague-Dawley (SD) rats, as measured by GOT, GPT, ALP and histological observation. The CF also showed significant decrease of malodialdehyde (MDA) and increase of glutathion (GSH), catalase activity in rat liver homogenate. In addition, the expression of CYP2E1, as measured by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analysis, was significantly decreased in the liver of CF treated SD rats. But CCl4 and CF has no significant effect on 1A1 and 3A1 isoform of cytochrome P450. Based on these findings, it is suggested that hepatoprotective effects of CF possibly related to antioxidative effects and regulation of CYP2E1 expression.

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