사염화탄소로 유발된 간손상에 대한 구기자 추출물의 치료효과

Abstract

The hepatotherapeutic effect of the extract of Lycii fructus has been studied in rats against CCl4 induced liver toxicity. The rats were orally treated with CCl4 (corn oil/CCl4 1:1, 1㎖/㎏) and then CCl4 (0.5㎖/㎏) administered four times for 2 weeks. The extracts of L. fructus have been administered every day for 2 weeks after the last CCl4 injection. The experimental groups consisted of negative control (G1), positive control (CCl4 alone; G2), extract of L. fructus (50 ㎎/㎏; G3, 100 ㎎/㎏; G4, 200 ㎎/㎏; G5), respectively. There was a significant decrement to G2 on the serum level of aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase in G5. Also, the content of thiobarbituric acid reactive substance, and phosphatidylcholine hydroperxidase, a marker of lipid peroxidation, in the liver were decreased significantly G5 and G4 compared with G2. Although, catalase or superoxide dismutase, antioxidant enzyme, in the liver were decreased significantly too, it would not be a good sign for the liver. In histopathological findings, such a hepatocellular vacuolar degeneration, lobular restructure, cellular infiltration, necrosis, and so on were shown severely in G2. However, G4 and G5 was shown a mild cytoplasmic vacuolation and inflammatory cell. In conclusion, as a protection against cell damage, lipid peroxidation and serum level, it suggested that the extract of Lycii fructus would have been a therapeutic effect of liver injury directly.