CCl4로 급성 간손상을 유도한 백서에서 人蔘葉과 莖抽出物의 간기능 개선과 항산화 작용
Improvement of Liver Function and Suppressed Lipid Peroxidation of Extract from Ginseng Folium and Stem in Acute CCl4 Intoxicated Rats
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Abstract
Panax ginseng is the one of best famous phytochemical plant in the world and it's various positive effects such as antioxidant, regulation of immunity are very well known. In this study, we investigated primary the cell viability and morphological change and secondary an antioxidative effect and liver function improvement of extract from Ginseng folium and stem in CCl4 intoxicated rats. The NCTC cell line were used for cell viability and sirius red staining before the animal experiment. The female Sprague-Dawley rats (90～100g) were divided into 3 groups (Normal, AC: CCl4 treated group, GFS: CCl4 + extract of Ginseng folium and stem treated group) and acute liver damage was developed by one time administration of CCl4 mixture (0.5 ㎖/rat). The liver tissue and sera were collected and used for quantitative measurement of enzyme activity (AST, ALT, ALP, BUN), MDA and Hyp. As a result, cell viability in GFS treated group (in concentration of 3.33～33.33 ㎎ GFS/200㎕ medium) was 180.9～241.0% significantly and dose dependently higher than in control group. And potential state of cell growth and differentiation and no criteria of cytoplasm lysis and nucleus breaking were observed in control and GFS group. The parameters of liver function (AST and ALP) in sera of GFS group showed significantly 93% and 67.6% lower than AC group (p<0.005～0.05). And the level of ALT and BUN showed fast similar in AC group and GFS group. The concentration of MDA in liver was decreased 576.5% significantly in GFS group when compared with AC group (p<0.005). The content of Hyp in GFS group is merely lower than in AC group. In conclusion, the water extract of Ginseng folium and stem such as Ginseng radix may be possessed the antioxidative effect and improvement of liver function in CCl4 intoxicated rats.
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