露蜂房이 t-butylhydroxyperoxide에 의한 Peroxynitrite 생성과 염증성 단백질 발현에 미치는 영향
Effects of Vespae Nidus on Peroxynitrite Production and Protein Expression of Proinflammatory Mediators
0
조회
0
다운로드
5
인용
0
참고문헌
초록
Abstract
Peroxynitrite (ONOO-) is a reactive oxidant formed from superoxide anion radical (·O2-) and nitric oxide (NO), which can oxidize cellular components such as essential protein, non-protein thiols, DNA, low-density lipoproteins and membrane phospholipids. ·O2- and ONOO- have contributed to the pathogenesis of diseases such as stroke, heart disease, Alzheimer's disease and atherosclerosis. Because of damaging effects of ·O2- and ONOO- oxidants, Vespae Nidus, which has been known to strengthen the kidneys to preserve the vital energy. was tested as a potential specific scavenger of those oxidants. In this study, the viability of Vespae Nidus (1, 10, 50 g/ml) to scavenge ·O2-, NO, ONOO- and so to protect cells against tert-butylhydroxyperoxide (t-BHP) induced cell death was tested. The levels of ·O2- and ONOO- were detected by staining with DCFH-DA and DHR 123, respectively. Protein expression levels of COX-2, iNOS and NF-κB were assayed by western blot. Vespae Nidus blocked t-BHP-induced cell death in a dose-dependent fashion. Vespae Nidus inhibited t-BHP-induced production of ·O2-, NO and ONOO- in YPEN cells. The lipid peroxide level was increased and glutathione level was decreased in lipopolysaccharide (LPS)-treated ICR mouse, whereas the ones in the Vespae Nidus-administered group were regulated beneficially. Vespae Nidus inhibited the expression of COX-2, iNOS and NF-κB (p65 and p50) genes in LPS-treated ICR mouse. The present study suggests that Vespae Nidus is a powerful antioxidant and promotes cellular defense activity by scavenging the toxic oxidants such as ·O2- and ONOO-.
참고문헌 (30)
- "Aging and oxidative stress: modulation by dietary restriction", 21, pp.651~668 (1996)
- "NO and NOS", 2, pp.207~214 (1996)
- "Oxidation of LDL by myeloperoxidase and reactive nitrogen species: reaction pathways and antioxidant protection", 20, pp.1716~1723 (2000)
- "The inflammatory process in aging", 10, pp.207~222 (2000)
- "Molecular inflammation hypothesis of aging based on the anti-aging mechanism of calorie restriction", 59, pp.264~272 (2002)
- "Peroxynitrite scavenging by flavonoids", 236, pp.591~596 (1997)
- "Inhibition of peroxynitrite-mediated tyrosine nitration by catechin polyphenols", 232, pp.164~170 (1997)
- "The effect of age on cyclooxygenase-2 gene expression : NF-κB activation and IκBα degradation", 28, pp.683~692 (2000)
- "NF-κB and Rel proteins : evolutionarity conserved mediators of immune responses", 16, pp.225~260 (1998)
- "Age-related changes in the regulation of transcription factor NF-kappa B in rat brain", 225, pp.61~64 (1997)
- "J. Free radical scavenging by ethanol extract of propolis. Int J Radiat Biol. 57", pp.461~465 (1990)
- "천연물질의 항균 활성에 관한 연구" (2001)
- "Propolis의 약물학적 특성. 항염증 및 에스트로젠성 효과" (2001)
- "Propolis가 마우스에서 종양 발생 및 비장세포와 대식세포의 성장에 미치는 영향" (1998)
- "露蜂房의 항산화 작용에 관한 연구", 13(1), pp.11~22 (2003)
- "K. An improved colorimetric assay for interleukin 2. J Immunol Methods. 93", pp.157~165 (1986)
- "B.N. Detection of picomole levels of hydroperoxides using a fluorescent dichlorofluorescein fluorescent assay. Anal Biochem. 134", pp.111~116 (1983)
- "J.S. Peroxynitrite-mediated oxidation of dihydrorhodamine 123. Free Radic Res Commun. 16", pp.149~156 (1994)
- "Inhibitory effects of catecholamines and anti-oxidants on the fluorescence reaction of 4,5-diaminofluorescein, DAF-2, a novel indicator of nitric oxide", 125, pp.658~661 (1999)
- "K. Assay for lipid peroxide in animal tissues by thiobarbituric acid reaction. Anal Biochem. 95", pp.351~358 (1979)
- "R.J. Protein measurement with folin phenol reagent. J Biol Chem. 193", pp.265~275 (1951)
- "M. Occupational and cosmetic dermatitis from propolis. Contact Dermatitis. 9", pp.163~164 (1983)
- "antiinflammatory and antipyretic studies on propolis bee products. J Ethnopharmacol. 35", pp.77~82 (1991)
- "D. Inhibition of tumor promoter-mediated processes in mouse skin and bovine lens by caffeic acid phenethylester. Cancer Res. 53", pp.1255~12561 (1993)
- "R.G. Scavenging action of propolis extract against oxygen radicals. J Ethnopharmacol. 41", pp.9~13 (1994)
- "프로폴리스에서 분리한 플라보노이드 화합물의 항산화 활성 및 방사선 방어 효과", 34(2), pp.162~166 (2005)
- "The Structure-Activity Relationship of Flavonoids as Scavengers of Peroxynitrite", 16, pp.232~235 (2002)
- "Moleculor inflammation hypothesis of aging based in the anti-aging mechanism of calorie restriction", 10, pp.991~1005 (2002)
- "J.F. The role of lipid peroxidation and biological antioxidants in the function of the adrenal cortex. Part 2. Mol Cell Endocrinol. 30", pp.123~147 (1983)
- "Mechanism of free radical induced toxicity and glutathione dependent protection. Pharmacol Ther. 37", pp.231~239 (1988)