The effects of Bee Venom on NO, H2O2 in Raw 264.7 cells and IL-1 in D10S cells
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Objectives : The purpose of this study was to investigate the effects of Bee Venom on NO, H2O2 expression induced by LPS in Raw 264.7 cells as a murine marcrophage cell line and on IL-1 expression induced by LPS in D10S cells. Methods : The expression of NO was measured by MTT Assay and IL-1 by MTS Assay. The expression of H2O2 was measured as ROS level within the cell using by FACS analysis. The non-toxic concentration(from 0.1ug/ml to 5ug/ml) of Bee Venom was determined by MTT Assay. Results : 1. Bee Venom inhibited the NO expression. The effective concentration of Bee Venom was 5ug/ml after 3 hours, 1 and 5ug/ml after 1 day and 2 days. The all concentration of Bee Venom inhibited the NO expression after 6, 12 hours and 3 days. 2. Bee Venom inhibited the H2O2 expression in a dose-dependent manner compared to the control. 3. Bee Venom could not significantly inhibit the IL-1 expression.
Abstract
Objectives : The purpose of this study was to investigate the effects of Bee Venom on NO, H2O2 expression induced by LPS in Raw 264.7 cells as a murine marcrophage cell line and on IL-1 expression induced by LPS in D10S cells. Methods : The expression of NO was measured by MTT Assay and IL-1 by MTS Assay. The expression of H2O2 was measured as ROS level within the cell using by FACS analysis. The non-toxic concentration(from 0.1ug/ml to 5ug/ml) of Bee Venom was determined by MTT Assay. Results : 1. Bee Venom inhibited the NO expression. The effective concentration of Bee Venom was 5ug/ml after 3 hours, 1 and 5ug/ml after 1 day and 2 days. The all concentration of Bee Venom inhibited the NO expression after 6, 12 hours and 3 days. 2. Bee Venom inhibited the H2O2 expression in a dose-dependent manner compared to the control. 3. Bee Venom could not significantly inhibit the IL-1 expression.