SD 랫드를 이용한 십전대보탕의 급성 독성 연구
Acute Toxicity Study on Sipjeondaebo-tang in Rats
1
조회
0
다운로드
15
인용
0
참고문헌
키워드
초록
Sipjeondaebo-tang has been traditionally prescribed a medicine as a restorative. In this study, we investigated the acute toxicity about water-extracted Sipjeondaebo-tang. Thirty rats completed 14 days of oral Sipjeondaebo-tang at the respective doses of 0(control group), 2000 and 5000 ㎎/kg. We observed survival rates, general toxicity, change of body weight and autopsy. To be confirmed the data for the toxicity and safety problems of oriental medicine prescription. Compared with the control group, we could not find any toxic alteration in all treated groups (2000 and 5000 ㎎/kg). LD50 of Sipjeondaebo-tang was over 5000 ㎎/kg and it is very safe to SD rats.
Abstract
Sipjeondaebo-tang has been traditionally prescribed a medicine as a restorative. In this study, we investigated the acute toxicity about water-extracted Sipjeondaebo-tang. Thirty rats completed 14 days of oral Sipjeondaebo-tang at the respective doses of 0(control group), 2000 and 5000 ㎎/kg. We observed survival rates, general toxicity, change of body weight and autopsy. To be confirmed the data for the toxicity and safety problems of oriental medicine prescription. Compared with the control group, we could not find any toxic alteration in all treated groups (2000 and 5000 ㎎/kg). LD50 of Sipjeondaebo-tang was over 5000 ㎎/kg and it is very safe to SD rats.
참고문헌 (19)
- 이상인, "방제학", pp.175~176 (1994), 도서출판 영림사
- Saiki, I., "A Kampo medicine ‘'Juzen-taiho-to’'-prevention of malignant progression and metastasis of tumor cells and the mechanism of action", Biol Pharm Bull, 23, pp.677~688 (2000)
- Ohnishi, Y., "Oral administration of a Kampo (Japanese herbal) medicine Juzen-taiho-to inhibits liver metastasis of colon 26-L5 carcinoma cells", Jpn. J. Cancer Re s, 89, pp.206~213 (1998)
- Saiki, I., "HPLC analysis of juzen-taiho-to and its variant formulations and their antimetastatic efficacies", Chem. Pharm. Bull, 47, pp.1170~1174 (1999)
- Matsumoto, T., "Orally administered decoction of Kampo (Japanese herbal) medicine, "Juzen-Taiho-To" modulates cytokine secretion and induces NKT cells in mouse liver", Immunopharmacol, 46, pp.149~161 (2000)
- Miyagami, M., "Improvement of host-immunity by adjuvant therapy with juzen-taiho-to for patients with brain tumors", No Shinkei Geka, 31, pp.401~409 (2003)
- Utsuyama, M., "Immunological restoration and anti-tumor effect by Japanese herbal medicine in aged mice", Mech. Ageing Dev, 122, pp.341~352 (2001)
- Ohnishi, Y., "Inhibitory effect of traditional Chinese medicine Juzentailio-to on progressive growth of weakly malignant clone cells derived from murine fibrosarcoma", Jpn J Cancer Res, 87, pp.1039~1044 (1996)
- Maruyama, H., "Antitumor effect of Juzen-taiho-to, a kampo medicine, combined with surgical excision of transplanted Meth-A fibrosarcoma", Int J Immunother, 9, pp.117~125 (1993)
- Zhang, Y.H., "Dissociated control by glycyrrhizin of proliferation and IL-2 production of murine thymocytes", Cell Immunol, 162, pp.97~104 (1995)
- Ohnish,i Y., "Inhibitory effect of traditional Chinese medicine Juzentailio-to on progressive growth of weakly malignant clone cells derived from murine fibrosarcoma", Jpn J Cancer Res, 87, pp.1039~1044 (1996)
- Hisha, H., "Isolation and identification of hematopoietic stem cell-stimulating substances from Kampo (Japanese herbal) medicine", Juzen-taiho-to. Blood, 90, pp.1022~1030 (1997)
- Dai, Y., "T-cell-immunity-based inhibitory effects of orally administered herbal medicine juzen-taiho-to on the growth of primarily developed melanocytic tumors in RET-transgenic mice", J Invest Dermatol, 117, pp.694~701 (2001)
- Kiyohara, H., "Characterization of in vitro IL-2-production-enhancing and anticomplementary pectic polysaccharides from Kampo (Japanese herbal) medicine Juzen-taiho-to", Phytotherapy Res, 7, pp.367~375 (1993)
- Iijima, K., "Juzen-taiho-to, a Japanese herbal medicine, modulates type-1 and type-2 T cell responses in old BALB", c mice, 1999, pp.27, 191~203
- Matsumoto, T., "Orally administered kampo (Japanese herbal) medicine Juzen-taiho-to modulates cytokine secretion in gut associated lymphoreticular tissues in mice", Phytomedicin, 6, pp.425~430 (2000)
- Matsumoto, T., "Orally dministered decoction of kampo (Japanese herbal) medicine Juzen-taiho-to modulates cytokine secretion and induces NKT cells in mouse liver", Immunophamacology, 46, pp.149~161 (2000)
- 식품의약품안전청, "의약품등의 독성시험방법", 식품의약품안전청고시. 의약품등의 독성시험방법. 제 2006 (60), ~ (2006)
- Hayes, A., Wallace, "Principles and Methods of Toxicology", pp.17~19 (1984), New York Raven press