Spargue-Dawley 랫드를 이용한 방풍통성산의 급성독성 연구

Abstract

Objectives : This study was conducted to evaluate the acute toxicity and safety of Bagpungtongsung-san (Fangfengtongsheng-san) in Sprague-Dawley rat though the current regulatory guideline. Methods : The preliminary study showed that the single oral administration of Bangpungtongsung-san (Fangfengtongsheng-san) did not induce any toxic effect at a dose level of 2000 ㎎/㎏. Based on the results,2000 ㎎/㎏ was selected as the limited dose. In this study, 10 rats of each sex were randomly assigned to two groups of 5 rats each and were administrated singly by gavage at dose levels of 0 and 2000 ㎎/㎏. Mortalities, clinical signs, and body weight changes were monitored for the 15-day period following administration. At the end of observation period, all animals were sacrificed and complete gross postmortem examinations were performed. Results : Throughout the study period, no treatment-related deaths were observed. There were no adverse effects on clinical signs, body weight, and gross findings at all treatment groups. Conclusions : These results showed that the single oral adminstration of Bangpungtongsung-san (Fangfengtongsheng-san) did not cause any toxic effect at the dose levels of 2000 ㎎/㎏ in rats. In conclusion, the LD50 of Bangpungtongsung-san (Fangfengtongsheng-san) was considered to be over 2000 ㎎/㎏ body for both sexes.