지모의 collagen 유발 관절염에 대한 소염 효과 -DBA/1J mouse 에서의 병태 관찰 및 RAW264.7에서의 cytokine 분비측정-
Anti-inflammatory Effect of Anemarrhenae Rhizoma on Collagen Induced Arthritis - a Model for Rheumatoid Rrthritis in DBA/1J Mice and Cytokine Production in Raw264.7 Cells
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In order to examine anti-inflammatory effect of Anemarrhenae Rhizoma (AR) alcohol extract on rheumatoid arthritis, the present study investigated the viability and TNF-α production in Raw264.7 cells treated with AR and collagen induced arthritis in DBA/1J mice which were orally administered with AR prior to immunization. The results are as follows: AR extract at 20 and 50 ㎍/ml inhibited the viability of Raw264.7 by 35% and 79%, respectively. AR showed a significant decrease in TNF-α levels from Raw264.7 cells treated with LPS. AR administration significantly decreased arthritic index in DBA/1J mice immunized with bovine collagen type Π. AR administration significantly decreased spleen weights obtained from mice in 6 weeks after immunization. AR administration significantly decreased serum anti-type Ⅱ collagen antibody levels compared with control group. AR administration decreased serum IL-6 levels compared with control group but it did not reach statistical significance.
Abstract
In order to examine anti-inflammatory effect of Anemarrhenae Rhizoma (AR) alcohol extract on rheumatoid arthritis, the present study investigated the viability and TNF-α production in Raw264.7 cells treated with AR and collagen induced arthritis in DBA/1J mice which were orally administered with AR prior to immunization. The results are as follows: AR extract at 20 and 50 ㎍/ml inhibited the viability of Raw264.7 by 35% and 79%, respectively. AR showed a significant decrease in TNF-α levels from Raw264.7 cells treated with LPS. AR administration significantly decreased arthritic index in DBA/1J mice immunized with bovine collagen type Π. AR administration significantly decreased spleen weights obtained from mice in 6 weeks after immunization. AR administration significantly decreased serum anti-type Ⅱ collagen antibody levels compared with control group. AR administration decreased serum IL-6 levels compared with control group but it did not reach statistical significance.
참고문헌 (23)
- Mindrescu, C., "Amelioration of collagen-induced arthritis in DBA", 1J mice by recombinant TSG-6, a tumor necrosis factor, Arthritis Rheum, pp.2000, 43[DOI]
- Y. Ono, "Suppressive Effect of Kanzo-bushi-to, a Kampo Medicine, on Collagen-induced arthritis", Biol. Pharm. Bull, 27(9), pp.1406~1413 (2004)
- Lipsky, P.E., "Eng. J. Med.", Eng. J. Med, 343, pp.1594~1602 (2000)
- Trentham, D.E., "Autoimmunity to type Ⅱ collagen: an experimental model of arthritis", J. Exp. Med, 146, pp.857~868 (1977)
- 이호근, "대강활탕이 제Ⅱ형 Collagen 유발 관절염의 항체에 미치는 영향", 한방재활의학과학회, 4(1), pp.87~99 (1994)
- 신병희, "계지작약지모탕이 제Ⅱ형 Collagen 유발 관절염의 항체에 미치는 영향", 한방재화의학과학회, 41, pp.121~133 (1994)
- 엄재원, "골담초가 제2형 collagen 유발 관절염의 항체에 미치는 영향", 한방재활의학과학회, 5(1), pp.149~161 (1995)
- 서영배, "동충하초가 synovial 세포 증식과 collagen에 의해 유발된 생쥐의 관절염 억제에 미치는 영향", 박사, pp.2000
- 설수용, "지모의 진정수면효과 및 장관평활근에 미치는 영향", 생약학회지, 16(1), pp.58~58 (1985)
- 도은수, "지모추출액의 항균활성과 항균성물질의 동정", 자연과연구논문집, 8(1), pp.59~70 (1999)
- 이승호, "지모의 항암활성성분에 관한 연구", 생약학회지, 26(1), pp.47~50 (1995)
- 대한정형외과학회지, "정형외과학", pp.175~175 (1995), 서울, 최신의학사
- Symmons, D.P, "What is rheumatoid arthritis?", Br Med Bull, 65(1), pp.273~281 (1995)
- 전국한의과대학 재활의학과교실, "동의재활의학과학", pp.95~96 (1995), 서울, 서원당
- 전국한의과대학 침구경혈학교실, "침구학(하)", pp.1294~1296 (1988), 서울, 집문당
- Holmdahl, R., "Type Ⅱ collagen autoimmunity in animals and provocations leading to arthritis", Immunol Rev, 118, pp.193~232 (1980)
- Tough, D.F., "Stimulation of naive and memory T cells by cytokines", Immunol Rev, 170, pp.39~47 (1999)
- Mosmann, T.R., "Two types of murine helper T cell clone. Definition according to profiles of lymphokine activities and secreted proteins", J Immunol, 136(7), pp.2348~2357 (1986)
- Holmdahl, R., "The molecular pathogenesis of collagen-induced arthritis in mice- a model for rheumatoid arthritis", Ageing Research Reviews, 1, pp.135~147 (2002)
- Terato, K., "Induction of arthritis with monoclonal antibodies to collagen", J Immunol, 148, pp.2103~2108 (1992)
- Seki, N., "Type Ⅱ collagen induced murine arthritis. Ⅰ. Induction and perpetuation of arthritis require synergy between humoral and cell-mediated immunity", J Immunol, 140, pp.1477~1484 (1988)
- Michaelsson, E., "Macrophages bt not dendritic cells present collagen to T cells", Eur J Immunol, 25, pp.2234~2241 (1995)
- Holmdahl, R., "Transgenic mouse models of rheumatoid arthritis", Immunol Rev, 169, pp.161~173 (1999)