관절9호방이 생쥐의 Collagen Ⅱ 유발 관절염에 미치는 영향
Inhibitory Effects of Gwanjul9-bang on Collagen Induced Arthritis in DBA/1J Mouse
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This study was carried out to know the effects of Gwanjul9-bang (hereinafter reffered to GJ9) on the inhibition of arthritis induced by collagen on DBA/1J mouse. For this purpose, GJ9 was orally administered to mouse with arthritis induced by collagen Ⅱ. Cytotoxicity, hepatotoxicity, arthritis index, value of immunocyte in draining lymph node and paw joint, rheumatoid factor (IgG, IgM) in serum were measured in vivo. The cytotoxicity against hFCs was not measured in any concentration. The hepatotoxicity was low in GJ9 treated group compared with MTX group. The arthritis index was decreased significantly. In total cell counts of DLN and paw joint, the cells in DLN increased significantly while there was significantly decrease in paw joint. In lymph nodes, CD19+, CD3+, CD4+, CD3+CD69+, CD8+, CD4+CD25+, CD3+CD49b+, CD4+CD44+, CD3+CD8+ cells increased significantly, B220+CD23+cells decreased significantly. In joints, CD3+, CD4+, CD4+CD25+, CD11b+Gr-1+ cells decreased significantly. The levels of IgG and IgM was significantly decreased compared with control. Anti-collagen Ⅱ in serum was significantly decreased compared with control. The degree of arthritis induced damage of joint of GJ9 group is slight compared with control group in histopathologic observation (Hematoxylin & Eosin, Masson's Trichrome). Comparison of the results for this study showed that GJ9 had immunomodulatory effects. So we expect that GJ9 should be used as a effective drugs for not only rheumatoid arthritis but also another auto-immune disease. Therefore we have to survey continuously in looking for the effective substance and mechanism in the future.
Abstract
This study was carried out to know the effects of Gwanjul9-bang (hereinafter reffered to GJ9) on the inhibition of arthritis induced by collagen on DBA/1J mouse. For this purpose, GJ9 was orally administered to mouse with arthritis induced by collagen Ⅱ. Cytotoxicity, hepatotoxicity, arthritis index, value of immunocyte in draining lymph node and paw joint, rheumatoid factor (IgG, IgM) in serum were measured in vivo. The cytotoxicity against hFCs was not measured in any concentration. The hepatotoxicity was low in GJ9 treated group compared with MTX group. The arthritis index was decreased significantly. In total cell counts of DLN and paw joint, the cells in DLN increased significantly while there was significantly decrease in paw joint. In lymph nodes, CD19+, CD3+, CD4+, CD3+CD69+, CD8+, CD4+CD25+, CD3+CD49b+, CD4+CD44+, CD3+CD8+ cells increased significantly, B220+CD23+cells decreased significantly. In joints, CD3+, CD4+, CD4+CD25+, CD11b+Gr-1+ cells decreased significantly. The levels of IgG and IgM was significantly decreased compared with control. Anti-collagen Ⅱ in serum was significantly decreased compared with control. The degree of arthritis induced damage of joint of GJ9 group is slight compared with control group in histopathologic observation (Hematoxylin & Eosin, Masson's Trichrome). Comparison of the results for this study showed that GJ9 had immunomodulatory effects. So we expect that GJ9 should be used as a effective drugs for not only rheumatoid arthritis but also another auto-immune disease. Therefore we have to survey continuously in looking for the effective substance and mechanism in the future.
참고문헌 (45)
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