길경배지 유산균이 마우스 대식세포의 NO 및 TNF-α에 미치는 영향
Effects of Lactobacillus Plantarum Cultured in Platycodi Radix Decoction on the Eexpression of NO and TNF-α in Mouse Macrophage RAW264.7 Cell Line
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
This study examined the effects of Lactobacillus plantarum(LP) cultured in Platycodi Radix decoction(LPPR) on the expressions of NO and TNF-α in mouse macrophage RAW264.7 cell line. Cells were stimulated with LP or LPPR (0.1, 1, and 10 bacteria/cell) in all assays. More NO was induced in LPPR than LPM at 0.1 and 1 of a LP: cell ratio. The iNOS mRNA expression was also enhanced in LPPR stimulated cells. TNF-α was increased in LPPR stimulated cells at the protein and mRNA level compared with LPM. In conclusion, LP fermented in Platycodi Radix decoction induced stronger activity in NO and TNF in mouse macrophages than LPM. These results suggest that fermentation by Platycodi Radix can be useful in enhancing the immunostimulatory activity of LP.
Abstract
This study examined the effects of Lactobacillus plantarum(LP) cultured in Platycodi Radix decoction(LPPR) on the expressions of NO and TNF-α in mouse macrophage RAW264.7 cell line. Cells were stimulated with LP or LPPR (0.1, 1, and 10 bacteria/cell) in all assays. More NO was induced in LPPR than LPM at 0.1 and 1 of a LP: cell ratio. The iNOS mRNA expression was also enhanced in LPPR stimulated cells. TNF-α was increased in LPPR stimulated cells at the protein and mRNA level compared with LPM. In conclusion, LP fermented in Platycodi Radix decoction induced stronger activity in NO and TNF in mouse macrophages than LPM. These results suggest that fermentation by Platycodi Radix can be useful in enhancing the immunostimulatory activity of LP.
참고문헌 (24)
- 민경찬, "필수 식품미생물학", pp.295~300 (2004), 광문각
- 심상국, "발효식품학", pp.11~ (2008), 진로연구사
- 신민교, "임상본초학", pp.749~780 (1997), 영림사
- 전국한의과대학 본초학교수, "본초학", pp.460~ (1998), 영림사
- 王大觀, "本草經義疏", pp.322~ (1995), 人民衛生出版社
- 李梴, "醫學入門", pp.165~ (1989), 고려의학
- 김호철, "한약약리학", pp.350~352 (2008), 집문당
- 이영은, "한방식품재료학", pp.152~153 (2004), 교문사
- 이정우, "유산균의 면역조절 효과 및 항종양 효과", 석사, pp.2003
- 김현주, "유산균 세포벽 성분의 항암 및 면역 활성에 관한 연구", 석사, pp.2004
- 공병만, "백삼, 홍삼, 발효인삼 농축액의 이화학적특성 및 약리효능에 관한 연구", 박사, 한방재료가공학과, pp.2008
- 조임식, "구기자(Lycium chinence Miller) 첨가에 따른 요구르트의 발효특성과 기능효과", 박사, pp.2004
- 강국희, "유산균식품학", pp.25~28 (1996), 성균관대학교출반부
- 방병호, "식품미생물학", pp.79~80 (2008), 진로
- Yun-Gi Kim, "Probiotic Lactobacillus casei activates innate Immunity via NF-κB and p38 MAP kinase signaling pathways", Microbes and Infection, 8, pp.994~1005 (2006)
- Janeway, Travers, "면역생물학", pp.13~ (2005), 라이프사이언스
- Abul K. Abbas, "세포분자면역학", pp.243~274 (2004), 범문사
- Trudy McKee, "생화학길라잡이", pp.418~ (2006), 라이프사이언스
- 곽한식, "생화학", pp.997~ (2008), 라이프사이언스
- Lubert Stryer, "생화학(하)", pp.686~687 (2003), (주)서울외국서적
- Lee, B.G, "Suppression of inducible nitric oxide synthase expression in RAW 264. 7 macrophages by two beta-carboline alkaloids extracted from Melia azedarach", Eur J Pharmacol, 406(3), pp.301~309 (2000)
- Chiou, W.F, "Camptothecin suppression nitric oxide biosynthesis in RAW 264.7 macrophage", Life Sci., 35, pp.625~635 (2001)
- Seo, W.G, "The aqueous extract of Rhodiola sachalinensis root enhances the expression of inducible nitric oxide synthase gene in RAW 264.7 macrophage", J Ethnopharmacol, 76, pp.119~123 (2001)