동충하초 균사체를 이용한 법제 유황의 항산화활성 및 항염증 효과
Study on the Anti-oxidative Activity and Anti-inflammatory Effects of Processed Sulfur with Cordyceps Militaris Mycelium
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The purpose of this study was evaluated physiological activity of processed sulfur with Cordyceps militaris mycelium that antioxidative and antiinflammatory effects. Proliferation of processed sulfur (PS) with Cordyceps militaris mycelium was increased in dose-dependent manner. In organic sulfur contents of Cordyceps militalis mycelium fortified processed sulfur, CM+PSH (CM+3000 ppm of PS) was significantly higher than other groups. However, CM+PSL (CM+1500 ppm of PS) was almost changed organic sulfur. Content of total polyphenol compounds was similarity to CM, CM+PSL and CM+PSH. The EDA (electron donating ability) and SOD-like activity was increased in dose-dependent manner and the activity of CM were significantly higher than CM+PSL and CM+PSH. We examined cytotoxicity, nitric oxide production of Raw 264.7 cell and inhibition of HT 1080 cell by MTT assay. CM, CM+PSL and CM+PSH do not have any toxic effects in macrophages (Raw 264.7). And CM+PSL and CM+PSH inhibited the production of nitrite in Raw 264.7 cells activated with LPS. The antitumor effects of processed sulfur with Cordyceps militaris mycelium on HT 1080 cell was indicated a significantly inhibition activity. These results suggested that processed sulfur with Cordyceps militaris mycelium have activities of antioxidant, antiinflammatory effects.
Abstract
The purpose of this study was evaluated physiological activity of processed sulfur with Cordyceps militaris mycelium that antioxidative and antiinflammatory effects. Proliferation of processed sulfur (PS) with Cordyceps militaris mycelium was increased in dose-dependent manner. In organic sulfur contents of Cordyceps militalis mycelium fortified processed sulfur, CM+PSH (CM+3000 ppm of PS) was significantly higher than other groups. However, CM+PSL (CM+1500 ppm of PS) was almost changed organic sulfur. Content of total polyphenol compounds was similarity to CM, CM+PSL and CM+PSH. The EDA (electron donating ability) and SOD-like activity was increased in dose-dependent manner and the activity of CM were significantly higher than CM+PSL and CM+PSH. We examined cytotoxicity, nitric oxide production of Raw 264.7 cell and inhibition of HT 1080 cell by MTT assay. CM, CM+PSL and CM+PSH do not have any toxic effects in macrophages (Raw 264.7). And CM+PSL and CM+PSH inhibited the production of nitrite in Raw 264.7 cells activated with LPS. The antitumor effects of processed sulfur with Cordyceps militaris mycelium on HT 1080 cell was indicated a significantly inhibition activity. These results suggested that processed sulfur with Cordyceps militaris mycelium have activities of antioxidant, antiinflammatory effects.
참고문헌 (31)
- Annual report on the cause of death statistics, "In National Statistical Office", Republic of Korea, ~ (2001)
- Kim, S.W, "Studies on the immunomodulating effects of polysaccharide extracted from Ganoderma lucidum on macrophage", J Korean Soc Food Sci Nutri, 26, pp.148~153 (1997)
- 류혜숙, "생강 추출물 투여가 마우스 면역세포 활성에 미치는 영향", 한국영양학회지, 37(1), pp.23~30 (2004), 한국영양학회
- Park, J.C, "Isolation and identification of the high-glutathione producing Saccharomyces cerevisiae FF-8 from korean traditional rice wine and optical producing conditions", J Korean Soc Agric Chem Biotechnol, 46, pp.348~352 (2003)
- 본초학교수, "본초학 6판", pp.639~640 (1991), 영림사
- 김성은, "법제 유황이 실험적 골 질환에 미치는 영향", 대한동의병리학회지, 10(1), pp.79~87 (1996)
- Barretine, B.F, "Salt and gypsum as regulators of cottonseed meal intake", pp.1958
- Bouchard, R, "Sulphur requirement of lactating dairy cows. H. Utilization of sulphates, molasses and lignin-sulfonate", J Dairy Sci, 56, pp.142~ (1973)
- 박찬성, "동충하초, 쑥 및 솔잎 추출물의 항균작용", 한국식품저장유통학회지, 9(1), pp.102~108 (2002), 한국식품저장유통학회
- 이상현, "동충하초 투여가 최대유산소성 능력과 피로회복에 미치는 영향", 한국체육교육학회지, 6(2), pp.1~9 (2002), 한국체육교육학회
- Kim, M.N, "Antioxidative and antimutagenic effect of the ethanol extract from Cordyceps militaris", Korean J Postharvest Sci Technol, 8(1), pp.109~117 (2001)
- 김재홍, "冬蟲夏草 藥鍼과 口腔投與가 Ovalbumin으로 유발된 Asthma에 미치는 영향", 대한침구학회지, 19(2), pp.39~50 (2002), 대한침구학회
- 김석종, "뽕나무가루 첨가 배지에서 배양한 버섯균사체 배양물의 자유라디칼 유도 산화 억제", 한국식품영양과학회지, 33(2), pp.255~261 (2004), 한국식품영양과학회
- 김현숙, "Cordyceps militaris에 함유된 혈당강하 성분이 간세포 Glucokinase 활성에 미치는 영향", 한국식품영양과학회지, 34(2), pp.158~161 (2005), 한국식품영양과학회
- 이성태, "토양중 게르마늄 농도에 따른 벼의 생육 특성 및 게르마늄 흡수", 한국환경농학회지, 24(1), pp.40~44 (2005), 한국환경농학회
- AOAC, "Official Methods of Analysis, 8th ed", pp.144~ (1985), Association of Official Analytical Chemists
- Blois, M.S, "Antioxidant determinations by the use of a stable free radical", J Agric Food Chem, 25, pp.103~107 (1977)
- Lee, B.G, "Suppression of inducible nitric oxide synthase expression in Raw 264.7 macrophages by two-carboline alkaloids extracted from Melia azedarach", Eur Pharmacol, 406, pp.301~309 (2000)
- 지형춘, "대한약전외 한약규격집주해서", pp.89~ (1988), 한국메디컬인덱스사
- 김기영, "한약포제학", pp.515~516 (1992), 신일상사
- Azuma, K, "Phenolic antioxidants from the leaves of Corchorus olitorius L", J Agric Food Chem, 47, pp.3963~3966 (1999)
- Ham, S.S, "Antimutagenic effects of juices from edible Korean wild herbs", J Food Sci Nutr, 2, pp.155~161 (1997)
- 이양숙, "구릿대 잎 추출물의 항산화 효과 및 생리활성", 한국식품저장유통학회지, 14(1), pp.78~86 (2007), 한국식품저장유통학회
- Nice, D.J, "Characterization of a heat stable antioxidant co purified with the superoxide dismutase activity from dried peas", Food Chem, 52, pp.393~397 (1995)
- Yamada, H, "Purification and characterization of complement activation acidic polysaccharide from the roots of Lithospermum euchromum royale", International J Immunopharmacology, 8, pp.71~82 (1986)
- Park, K.M, "Nitric oxide in involved in the immunomodulating activities of acidic polysaccharide from Panax ginseng", Planta Medica, 67, pp.122~126 (2001)
- Park, J.D, "Recent studied on the chemical constituents of Korean ginseng(Panax ginseng CA Meyer)", Korean J Gingseng Sci, 20, pp.389~396 (1996)
- Nathan, C, "Nitric oxide as a secretory product of mammalian cells", FASEB J, 6, pp.3051~3064 (1992)
- Epe, B, "DNA damage by peroxynitrite characterized with DNA repair enzyme", Nucleic acids Fes, 24, pp.4105~4110 (1996)
- Palmer, R.M, "Vascular endothelial cells synthesize nitric oxide from L-arginine", Nature, 333, pp.664~666 (1988)
- Park, J.G, "Chemosensitivity testing of human colorectal carcinoma cell lines using a tetrazolium-based colorimetric assay", Cancer Res, 47, pp.5875~5879 (1987)