Sprague-Dawley 랫드를 이용한 소청룡탕의 단회투여독성시험
Single Dose Toxicity Study of Socheongryong-tang in Sprague-Dawley Rats
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Socheongryong-tang has been used for the treatment of inflammatory allergic diseases such as allergic rhinitis and bronchial asthma in Asian countries. This study was conducted to investigate the safety of Socheongryong-tang in rats. The safety of this tang on acute toxicity was evaluated by single dose toxicity study. Rats were orally administrated in a single dose of 0 and 2000 mg/kg (limited dose) Socheongryong-tang. There were 7 rats in each groups. All animals were sacrificed after 14 days of treatment. After single administration, mortality, clinlcal signs, body weight changes and gross pathological findings were observed for 14 days. Three parameters were tested: organ weight measurement, clinical chemistry, and hematology. In this study with rats, Socheongryong-tang treatment did not show any acute toxicity. No mortality was noted for 14 days of treatment. There were no adverse effects on clinical signs, body weight, organ weight and gross pathological findings at all treatment groups. The clinical chemistry parameters attesting to liver and kidney functions as well as the hematological parameters were within the normal ranges. From single dose toxicity study with rats, it is considered that LD_50 of Socheongryong-tang is over 2000 mg/kg in oral administration. This finding of the safety on single dose toxicity study of Socheongryong-tang are expected to strengthen the position of Socheongryong-tang as nontoxic medicine.
Abstract
Socheongryong-tang has been used for the treatment of inflammatory allergic diseases such as allergic rhinitis and bronchial asthma in Asian countries. This study was conducted to investigate the safety of Socheongryong-tang in rats. The safety of this tang on acute toxicity was evaluated by single dose toxicity study. Rats were orally administrated in a single dose of 0 and 2000 mg/kg (limited dose) Socheongryong-tang. There were 7 rats in each groups. All animals were sacrificed after 14 days of treatment. After single administration, mortality, clinlcal signs, body weight changes and gross pathological findings were observed for 14 days. Three parameters were tested: organ weight measurement, clinical chemistry, and hematology. In this study with rats, Socheongryong-tang treatment did not show any acute toxicity. No mortality was noted for 14 days of treatment. There were no adverse effects on clinical signs, body weight, organ weight and gross pathological findings at all treatment groups. The clinical chemistry parameters attesting to liver and kidney functions as well as the hematological parameters were within the normal ranges. From single dose toxicity study with rats, it is considered that LD_50 of Socheongryong-tang is over 2000 mg/kg in oral administration. This finding of the safety on single dose toxicity study of Socheongryong-tang are expected to strengthen the position of Socheongryong-tang as nontoxic medicine.
참고문헌 (20)
- Ko, E.J., "So-Cheong-Ryong-Tang, traditional Korean medicine, suppresses Th2 lineage development", Biol Pharm Bull, 27(5), pp.739~743 (2004)
- Ko, E.J., "Traditional Korean medicine (SCRT) modulate Th1", Th2 specific cytokine production in mice CD4+ T cell, 2004, pp.91, 121~128
- 동의학연구소, "새로보는 방약합편", pp.236~252 (2006), 도서출판 단샘
- 허 준, "신대역 동의보감", pp.1091~ (2009), 법인문화사
- Nakao, M., "The effect of Shoseiryuto, a traditional Japanese medicine, on cytochrome P450s, N-acetyltransferase 2 and xanthine oxidase, in extensive or intermediate metabolizers of CYP2D6", Eur J Clin Pharmacol, 63, pp.345~353 (2007)
- Kao, S.T., "The Effect of herbal medicine, xiao-qing-long-tang (XQLT), on allergen-induced bronchial inflammation in mite-sensitized mice", Allergy, 55, pp.1127~1130 (2000)
- Kao, S.T., "Effects of xiao-qing-long-tang (XQLT) on bronchoconstriction and airway eosinophil infiltration in ovalbumin-sensitized guinea pigs: in vivo and in vitro studies", Allergy, 56, pp.1164~1171 (2001)
- Bruchajzer, E., "Acute and subacute nephrotoxicity of 2-bromophenol in rats", Toxicol Lett, 134, pp.245~252 (2002)
- Fujii, H., "Evaluation of the safety and toxicity of the oligomerized polyphenol oligonol", Food Chem Toxicol, 45, pp.378~387 (2007)
- Harizal, S.N., "Acute toxicity study of the standardized methanolic extract of Mitragyna speciosa Korth in Rodent", J Ethnopharmacol, 131, pp.404~409 (2010)
- Mukinda, J.T., "Acute and cronic toxicity of the aqueous extract of Artemisia afra in Rodent", J Ethnopharmacol, 111, pp.138~144 (2007)
- Sheng, Y., "Enhanced DNA repair, immune function and reduced toxicity of C-MED-100TM, a novel aqueous extract from Uncaria tomentosa", J Ethnopharmacol, 69, pp.115~126 (2000)
- Topping, D.C., "Hydroquinone: Acute and subchronic toxicity studies with emphasis on neurobehavioral and nephrotoxic effects", Food Chem Toxicol, 45, pp.70~78 (2007)
- 식품의약품안전청, "식품의약품안전청고시 제 2009-116호(2009년 08월 24일) 의약품등의 독성시험기준", ~ (2009)
- 식품의약품안전청, "식품의약품안전청고시 제 2009-102호(2009년 08월 24일) ‘비임상시험관리기준’", ~ (2009)
- OECD, "OECD guidelines for testing of chemical no. 423. Acute oral toxicity-Acute class method, line 23", ~ (2001)
- 김은순, "랫트에서 한약재 복합물 BDR-29의 단회 경구투여 독성에 관한 연구", 생약학회지, 38(1), pp.71~75 (2007), 한국생약학회
- 신인식, "Spargue-Dawley 랫드를 이용한 평위산의 안전성 연구", 동의생리병리학회지, 24(3), pp.426~429 (2010), 대한동의병리학회
- 정하숙, "생리활성이 우수한 고품질 유색미의 단회 경구투여 안전성 연구", 한국식품영양과학회지, 36(5), pp.527~533 (2007), 한국식품영양과학회
- 김성민, "섬백리향 정유의 진통, 항염증, 정신적인 작용", 약 학 회 지, 51(6), pp.508~516 (2007), 대한약학회