눈연꽃 추출물의 Lyn과 Syk 인산화 억제를 통한 알레르기성 염증 억제 효과
Reduction of Allergic Inflammation Water Extract of Saussurea involucrata (Kar. & Kir.) Sch. Bip. through Tyrosine-protein Kinase Lyn and Sky Tyrosine Phosphorylation
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Background: Snow lotus [Saussurea involucrata (Kar. & Kir.) Sch. Bip.] is used in Chinese herbal medicine because of its antioxidant and anti-inflammatory effects. The mechanism of its antiinflammatory effect on allergic response is unknown. Methods and Results: We evaluated the effect of the aqueous extract of S. involucrata flowers on the allergic inflammatory response in dinitrophenyl-immunoglobulin E (DNP-IgE)-activated RBL- 2H3 cells. Cell viability and β-hexosaminidase release from the activated RBL-2H3 cells were evaluated. The levels of proinflammatory molecules including tumor necrosis factor-alpha (TNF- α), interleukin (IL)-4, IL-10, IL-1β, and prostaglandin E2 in RBL-2H3 cells treated with 5% and 10% S. involucrata extract. Treatment with S. involucrata extract significantly suppressed the phosphorylation of protein kinase B (AKT), p38, c-Jun N-terminal kinase (JNK), extracellular signal- regulated kinase (ERK)1/2, and mitogen-activiated protein kinase (MEK) in a dose-dependent manner. S. involucrata extract also inhibited the Fc epsilon receptor 1 signaling pathway involving phosphorylation of Lyn, Syk, and phosphoinositide 3-kinase (PI3K), which is associated with the PI3K-AKT-ERK pathway and cytokine expression. A major mechanism of action of S. involucrata extract was to reduce the extent of the DNP-IgE-induced cytoskeletal change from globular to filamentous actin in RBL-2H3 cells. Conclusions: S. involucrata extract mediates its antiallergic effect by suppressing inflammation. The findings will be valuable in the development of S. involucrata extract as a phytomedicine with minimal side effects for allergic diseases.
Abstract
Background: Snow lotus [Saussurea involucrata (Kar. & Kir.) Sch. Bip.] is used in Chinese herbal medicine because of its antioxidant and anti-inflammatory effects. The mechanism of its antiinflammatory effect on allergic response is unknown. Methods and Results: We evaluated the effect of the aqueous extract of S. involucrata flowers on the allergic inflammatory response in dinitrophenyl-immunoglobulin E (DNP-IgE)-activated RBL- 2H3 cells. Cell viability and β-hexosaminidase release from the activated RBL-2H3 cells were evaluated. The levels of proinflammatory molecules including tumor necrosis factor-alpha (TNF- α), interleukin (IL)-4, IL-10, IL-1β, and prostaglandin E2 in RBL-2H3 cells treated with 5% and 10% S. involucrata extract. Treatment with S. involucrata extract significantly suppressed the phosphorylation of protein kinase B (AKT), p38, c-Jun N-terminal kinase (JNK), extracellular signal- regulated kinase (ERK)1/2, and mitogen-activiated protein kinase (MEK) in a dose-dependent manner. S. involucrata extract also inhibited the Fc epsilon receptor 1 signaling pathway involving phosphorylation of Lyn, Syk, and phosphoinositide 3-kinase (PI3K), which is associated with the PI3K-AKT-ERK pathway and cytokine expression. A major mechanism of action of S. involucrata extract was to reduce the extent of the DNP-IgE-induced cytoskeletal change from globular to filamentous actin in RBL-2H3 cells. Conclusions: S. involucrata extract mediates its antiallergic effect by suppressing inflammation. The findings will be valuable in the development of S. involucrata extract as a phytomedicine with minimal side effects for allergic diseases.
참고문헌 (32)
- Baird B, "How does the actin cytoskeleton regulate distribution and diffusion of membrane components?", Biophysical Journal, 118, pp.15a~ (2020)[DOI]
- Thi Tho Bui, "Baicalein, wogonin, and Scutellaria baicalensis ethanol extract alleviate ovalbumin-induced allergic airway inflammation and mast cell-mediated anaphylactic shock by regulation of Th1", Th2 imbalance and histamine release, 2017, pp.50(2), 124~134[DOI]
- Bundgaard L, "Evaluation of systemic and local inflammatory parameters and manifestations of pain in an equine experimental wound model", Journal of Equine Veterinary Science, 68, pp.81~87 (2018)
- Draberova L, "Cytoskeletal protein 4.1R is a positive regulator of the fcεri signaling and chemotaxis in mast cells", Frontiers in Immunology, 10, pp.3068~ (2019)[DOI]
- Kun Guo, "Hesperidin Protects Against Intestinal Inflammation by Restoring Intestinal Barrier Function and Up‐Regulating Treg Cells", Molecular Nutrition & Food Research, 64(10), pp.1970058~ (2020), Wiley[DOI]
- Hagman S, "Effects of inflammatory cytokines ifn-γ, tnf-α and il-6 on the viability and functionality of human pluripotent stem cell-derived neural cells", Journal of neuroimmunology, 331, pp.36~45 (2019)
- Mohammad I Hirzallah, "The Monitoring of Brain Edema and Intracranial Hypertension", Journal of Neurocritical Care, 9(2), pp.92~104 (2016), 대한신경집중치료학회[DOI]
- Kawanishi S, "Crosstalk between DNA damage and inflammation in the multiple steps of carcinogenesis", International Journal of Molecular Sciences, 18, pp.1808~ (2017)[DOI]
- Kim JI, "During adipocyte remodeling, lipid droplet configurations regulate insulin sensitivity through F-actin and G-actin reorganization", Molecular and Cellular Biology, 39, pp.e00210~e00219 (2019)[DOI]
- Kim SR, "Rapid repigmentation of vitiligo using tofacitinib plus low-dose, narrowband uv-b phototherapy", JAMA Dermatology, 154, pp.370~371 (2018)
- Kim YY, "2-hydroxy-3-methoxybenzoic acid attenuates mast cell-mediated allergic reaction in mice via modulation of the fcεri signaling pathway", Acta Pharmaceutica Sinica, 38, pp.90~99 (2017)
- Kong ZL, "In vitro anti-inflammatory effects of curcumin on mast cell-mediated allergic responses via inhibiting fcεri protein expression and protein kinase c delta translocation", Cytotechnology, 72, pp.81~95 (2020)
- Larsen LF, "A comparative study on basophil activation test, histamine release assay, and passive sensitization histamine release assay in the diagnosis of peanut allergy", Allergy, 73, pp.137~144 (2018)
- 이영민, "Antioxidative, Anti-inflammatory, and DNA Damage Protective Effect in Cortex Extracts of Eucommia ulmoides by Roasting", 한국약용작물학회지, 27(4), pp.259~270 (2019), 한국약용작물학회[DOI]
- Lin K, "Inhibition of ampk through lyn-syk-akt enhances fcεri signal pathways for allergic response", Journal of Molecular Medicine, 94, pp.183~194 (2016)
- Liou C, "Acacetin from traditionally used saussurea involucrata kar. Et kir. Suppressed adipogenesis in 3t3-l1 adipocytes and attenuated lipid accumulation in obese mice", Frontiers in Pharmacology, 8, pp.589~ (2017)[DOI]
- Lodhi S, "Protective effects of luteolin on injury induced inflammation through reduction of tissue uric acid and pro-inflammatory cytokines in rats", Journal of Traditional and Complementary Medicine, 10, pp.60~69 (2020)
- Méndez-Pertuz M, "Modulation of telomere protection by the pi3k", akt pathway, 2017, pp.8, 1278~
- Pang L, "Apigenin attenuates pm2. 5-induced airway hyperresponsiveness and inflammation by down-regulating NF-κB in murine model of asthma", International Journal of Clinical and Experimental Pathology, 12, pp.3700~3709 (2019)
- Park JB, "Taeumjowi-tang, a traditional korean Sasang remedy, improves obesity-atopic dermatitis comorbidity by regulating hypoxia-inducible factor 1 alpha", Frontiers in Pharmacology, 10, pp.1458~ (2019)[DOI]
- A. Patruno, "Extremely low-frequency electromagnetic fields accelerates wound healing modulating MMP-9 and inflammatory cytokines", Cell Proliferation, 51(2), pp.e12432~ (2018), Wiley[DOI]
- Seresirikachorn K, "Leukotriene receptor antagonist addition to H1-antihistamine is effective for treating allergic rhinitis : A systematic review and meta-analysis", American Journal of Rhinology and Allergy, 33, pp.591~600 (2019)
- Štromberga Z, "The role of histamine in modulating urothelial contractile activity", Australian and New Zealand Continence Journal, 23, pp.112~112 (2017)
- Tabakawa Y, "Histamine released from skin-infiltrating basophils but not mast cells is crucial for acquired tick resistance in mice", Frontiers in Immunology, 9, pp.1540~ (2018)[DOI]
- Line K. Tannert, "Is a positive intracutaneous test induced by penicillin mediated by histamine? A cutaneous microdialysis study in penicillin-allergic patients", Clinical and Translational Allergy, 7(1) (2017), Wiley[DOI]
- Tiboc-Schnell CN, "Quercetin attenuates naso-sinusal inflammation and inflammatory response in lungs and brain on an experimental model of acute rhinosinusitis in rats", Journal of Physiology and Pharmacology, 71, pp.479~490 (2020)
- Wang P, "Limitations of mtt and mts-based assays for measurement of antiproliferative activity of green tea polyphenols", PloS one, 5, pp.e10202~ (2010)[DOI]
- Wang R, "Characterization of the complete chloroplast genome of saussurea involuerata(compositae), an endangered species endemic to china", Mitochondrial DNA Part B Resources, 5, pp.511~512 (2020)
- Xu N, "Formononetin ameliorates mast cell-mediated allergic inflammation via inhibition of histamine release and production of pro-inflammatory cytokines", Experimental and Therapeutic Medicine, 14, pp.6201~6206 (2017)
- Ye J, "Polydatin inhibits mast cellmediated allergic inflammation by targeting PI3K", Akt, MAPK, NF-κB and Nrf2, Scientific Reports, pp.2017, 7[DOI]
- Zhang L, "A Cu", Zn superoxide dismutase gene from Saussurea involucrata Kar. & Kir., SICSD, enhances drought, cold, and oxidative stress in transgenic tobacco, 2017, pp.97, 816~826
- Zhang W, "Modeling Pichia pastoris growth on methanol and optimizing the production of a recombinant protein, the heavy-chain fragment C of botulinum neurotoxin, serotype A", Biotechnology and Bioengineering, 70, pp.1~8 (2000)[DOI]