본문 바로가기
KERD
논문KCI 등재

오미자의 유방암 세포사멸과 TRPM7 관련성에 관한 연구

Effects of Schisandra Chinensis on Human Breast Cancer Cells

6

조회

0

다운로드

8

인용

0

참고문헌

키워드

주제분류한의학

초록

Fruits of Schisandra chinensis (SC) Baill are considered a traditional herbal medicine for the treatment and alleviation of various diseases. The purpose of this study was to investigate the anti-cancer effects of SC extract in human breast adenocarcinoma cells (MCF-7). We used human breast adenocarcinoma cell line, MCF-7 cells. We examined cell death by MTT assay and caspase 3 and 9 assay with SC extract. To examine the inhibitory effects of SC extract, cell cycle (sub G1) analysis and mitochondrial membrane depolarization was done the MCF-7 cells after one day with SC extract. In addition, to investigate the transient receptor potential melastatin 7 (TRPM7) currents, we used the whole cell patch clamp techniques. Furthermore, TRPM7 channels were overexpressed in human embryonic kidney (HEK) 293 cells to identify the role of TRPM7 channels in MCF-7 cell growth and survival. SC extract inhibited the growth of MCF-7 cells in a dose-dependent fashion. Also we showed that SC extract induced apoptosis in MCF-7 cells by MTT assay, caspase 3 and 9 assay, sub-G1 analysis and mitochondrial membrane depolarization. SC extract inhibited the TRPM7 currents in MCF-7 cells and in TRPM7 overexpressed HEK 293 cells. Furthermore, TRPM7 channel overexpression in HEK 293 cells exacerbated SC extract-induced cell death. Our findings provide insight into unraveling the effects of SC extract in human breast adenocarcinoma cells and developing therapeutic agents against breast cancer.

Abstract

Fruits of Schisandra chinensis (SC) Baill are considered a traditional herbal medicine for the treatment and alleviation of various diseases. The purpose of this study was to investigate the anti-cancer effects of SC extract in human breast adenocarcinoma cells (MCF-7). We used human breast adenocarcinoma cell line, MCF-7 cells. We examined cell death by MTT assay and caspase 3 and 9 assay with SC extract. To examine the inhibitory effects of SC extract, cell cycle (sub G1) analysis and mitochondrial membrane depolarization was done the MCF-7 cells after one day with SC extract. In addition, to investigate the transient receptor potential melastatin 7 (TRPM7) currents, we used the whole cell patch clamp techniques. Furthermore, TRPM7 channels were overexpressed in human embryonic kidney (HEK) 293 cells to identify the role of TRPM7 channels in MCF-7 cell growth and survival. SC extract inhibited the growth of MCF-7 cells in a dose-dependent fashion. Also we showed that SC extract induced apoptosis in MCF-7 cells by MTT assay, caspase 3 and 9 assay, sub-G1 analysis and mitochondrial membrane depolarization. SC extract inhibited the TRPM7 currents in MCF-7 cells and in TRPM7 overexpressed HEK 293 cells. Furthermore, TRPM7 channel overexpression in HEK 293 cells exacerbated SC extract-induced cell death. Our findings provide insight into unraveling the effects of SC extract in human breast adenocarcinoma cells and developing therapeutic agents against breast cancer.

참고문헌 (47)

  1. Scaffidi, C., "Two CD95 (APO-1", Fas) signaling pathways, 1998, pp.17, 1675~1687
  2. Lawen, A., "Apoptosis - an introduction", Bioessays, 25, pp.888~896 (2003)
  3. Jin, Z., "Overview of cell death signaling pathways", Cancer Biol Ther, 4, pp.139~163 (2005)
  4. Kroemer, G., "Mitochondrial control of cell death", Nat Med, 6, pp.513~519 (2000)
  5. Liu, X., "Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c", Cell, 86, pp.147~157 (1996)
  6. 한송이, "홍화가 인체 대장암세포에 미치는 효과", 한국한의학연구원논문집, 17(2), pp.129~134 (2011), 한국한의학연구원
  7. 김정아, "홍화가 인체 위암세포에 미치는 효과", 동의생리병리학회지, 25(3), pp.466~470 (2011), 대한동의병리학회
  8. Kim, B. J., "Transient Receptor Potential Melastatin 7 Channels are Involved in Ginsenoside Rg3-Induced Apoptosis in Gastric Cancer Cells", Basic & Clinical Pharmacology & Toxicology, 109, pp.233~239 (2011)
  9. 이희정, "고삼의 인체 유방암세포에 미치는 효과", 한국한의학연구원논문집, 18(1), pp.75~84 (2012), 한국한의학연구원
  10. 임보라, "인체 위암세포에서 고삼의 세포사멸효과", 한국한의학연구원논문집, 18(1), pp.85~92 (2012), 한국한의학연구원
  11. 성기천, "천연 오미자 추출물의 약리, 화학적 특성 및 분석", 韓國油化學會誌, 28(3), pp.290~298 (2011), 한국유화학회
  12. 조영제, "오미자(Schizandra chinensis Baillon) 추출물의 생리활성", Applied Biological Chemistry, 50(3), pp.198~203 (2007), 한국응용생명화학회
  13. 김현구, "오미자 추출물의 추출특성 및 항산화 효과", 한국식생활문화학회지, 19(5), pp.484~490 (2004), 한국식생활문화학회
  14. Lee, M. S., "Schizandrin protects primary rat cortical cell cultures from glutamate-induced apoptosis by inhibiting activation of the MAPK family and the mitochondria dependent pathway", Molecules, 18, pp.354~372 (2012)
  15. Melhem, A., "Treatment of chronic hepatitis C virus infection via antioxidants: results of a phase I clinical trial", J Clin Gastroenterol, 39, pp.737~742 (2005)
  16. Park, E. J., "Schisandrin B suppresses TGFβ1 signaling by inhibiting Smad2", 3 and MAPK pathways, 2012, pp.83, 378~384
  17. Jo, S. H., "In vitro and in vivo anti-hyperglycemic effects of Omija (Schizandra chinensis) fruit", Int J Mol Sci, 12, pp.1359~1370 (2011)
  18. 박성혜, "기능성 식품으로의 활용을 위한 한약자원에 관한 연구. 1. 오미자 열수추출물이 흰쥐의 국소 뇌혈류량과 혈압에 미치는 영향", 한국식품영양과학회지, 33(1), pp.34~40 (2004), 한국식품영양과학회
  19. 이성환, "陽陵泉 五味子약침이 고지방식이로 유발된 고지혈증 흰쥐에 미치는 영향", 대한침구의학회지, 28(2), pp.143~153 (2011), 대한침구의학회
  20. Gnabre, J., "Isolation of lignans from Schisandra chinensis with anti-proliferative activity in human colorectal carcinoma: Structure-activity relationships", Analyt Technol, 878, pp.2693~2700 (2010)
  21. Kim, J. H., "Apoptosis induction of human leukemia U937 cells by gomisin N, a dibenzocyclooctadiene lignan, isolated from Schizandra chinensis Baill", Food Chem Toxicol, 48(3), pp.807~813 (2010)
  22. Kim, B. J., "Suppression of transient receptor potential melastatin 7 channel induces cell death in gastric cancer", Cancer Sci, 99(12), pp.2502~2509 (2008)
  23. Guilbert, A., "Evidence that TRPM7 is required for breast cancer cell proliferation", Am J Physiol Cell Physiol, 297(3), pp.C493~C502 (2009)
  24. Wykes, R.C., "Functional transient receptor potential melastatin 7 channels are critical for human mast cell survival", J Immunol, 179(6), pp.4045~4052 (2007)
  25. Jiang, J., "Transient receptor potential melastatin 7-like current in human head and neck carcinoma cells: role in cell proliferation", Cancer Res, 67(22), pp.10929~10938 (2007)
  26. Abed, E., "Importance of melastatin-like tran\-sient receptor potential 7 and cations (magnesium, calcium) in human osteoblast-like cell proliferation", Cell Prolif, 40(6), pp.849~865 (2007)
  27. National survey of cancer patients; Analysis of national cancer survey, "Ministry of Health and Welfare", ~ (2008)
  28. Kelsey, J.L., "Breast cancer epidemiology", Cancer Res, 48, pp.5615~5623 (1988)
  29. Nkondjock, A., "Risk factors and risk reduction of breast cancer", Med Sci (Paris), 21, pp.175~180 (2005)
  30. Dorai, T., "Role of chemopreventive agents in cancer therapy", Cancer Lett, 215, pp.129~140 (2004)
  31. 대한내과학회, "Harrison’s 내과학", pp.559~567 (2006), 도서출판 MIP
  32. 권은미, "알러젠 제거 옻나무 추출물 위주의 한방치료와 항암화학요법을 병용한 폐암 환자 2례", 대한암한의학회지, 14(1), pp.13~20 (2009)
  33. Kim, O.C., "Volatile components of Schizandra chinensis Baillon", Kor J Agric Chem Biotech, 39, pp.30~36 (1994)
  34. Panossian, A., "Pharmacology of Schisandra chinensis Bail.: an overview of Russian research and uses in medicine", J Ethnopharmacol, 118(2), pp.183~212 (2008)
  35. "국민건강보험공단"[URL]
  36. 이석준, "환자들의 정보요구가 e-Health 시스템 사용과 성과에 미치는 영향에 관한 연구: 유방암환자대상 수요자의료정보시스템을 중심으로", 정보시스템연구, 22(2), pp.105~129 (2013), 한국정보시스템학회
  37. Clapham, D.E., "TRP channels as cellular sensors", Nature, 426(6966), pp.517~524 (2003)
  38. Vannier, B., "The membrane topology of human transient receptor potential 3 as inferred from glycosylation scanning mutagenesis and epitope immunocytochemistry", J Biol Chem, 273(15), pp.8675~8679 (1998)
  39. Pedersen, S.F., "TRP channels: an overview", Cell Calcium, 38(3), pp.233~252 (2005)
  40. Schmitz, C., "Regulation of vertebrate cellular Mg2+ homeostasis by TRPM7", Cell, 114, pp.191~200 (2003)
  41. Aarts, M., "A key role for TRPM7 channels in anoxic neuronal death", Cell, 115, pp.863~877 (2003)
  42. Elizondo, M.R., "Defective skeletogenesis with kidney stone formation in dwarf zebrafish mutant for trpm7", Curr Biol, 15, pp.667~671 (2005)
  43. Vriens, J., "Invertebrate TRP proteins as functional models for mammalian channels", Pflugers Arch, 449, pp.213~226 (2004)
  44. Sahni, J., "TRPM7 ion channels are required for sustained phosphoinositide 3-kinase signaling in lymphocytes", Cell Metab, 8, pp.84~93 (2008)
  45. Kim, B.J, , Gastroenterology, 129, pp.1504~1517 (2005)

관련 논문AI추천

이 간행물의 다른 논문 (15건)