Cosmetic Potency of Puerariae Radix in Dermal Fibroblasts
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Interaction between epidermis and dermis plays an important role in wound healing and hair follicle formation. This study focused on investigating the potency of ethanol extract of Puerariae Radix (EPR) as cosmetic ingredient using human dermal fibroblasts (hDFn). Our results revealed that EPR suppressed collagenase activity dose-dependently. EPR inhibited activity of 5α-reductase I and II at the final concentration of 25 μg/ml in hDFn cells. Also, EPR promoted the proliferation and the ERK activation of cells. ERK phosphorylation by EPR was blocked by specific inhibitor of ERK, PD98059. EPR-induced cell proliferation was blocked by PD98059. This means that EPR could promote the proliferation of hDFn cells via the activation ERK. Collectively, these results suggest that EPR may be used as a new cosmetic ingredient.
Abstract
Interaction between epidermis and dermis plays an important role in wound healing and hair follicle formation. This study focused on investigating the potency of ethanol extract of Puerariae Radix (EPR) as cosmetic ingredient using human dermal fibroblasts (hDFn). Our results revealed that EPR suppressed collagenase activity dose-dependently. EPR inhibited activity of 5α-reductase I and II at the final concentration of 25 μg/ml in hDFn cells. Also, EPR promoted the proliferation and the ERK activation of cells. ERK phosphorylation by EPR was blocked by specific inhibitor of ERK, PD98059. EPR-induced cell proliferation was blocked by PD98059. This means that EPR could promote the proliferation of hDFn cells via the activation ERK. Collectively, these results suggest that EPR may be used as a new cosmetic ingredient.
참고문헌 (26)
- Hogan BL, "Morphogenesis", Cell, 96, pp.225~233 (1999)
- Hogan BL, "Organogenesis : Molecular Mechanisms of Tubulogenesis", Nat Rev Genet, 3, pp.513~523 (2002)
- Tracy LE, "Extracellular matrix and dermal fibroblast function in the healing wound", Adv Wound Care(New Rochelle), 5, pp.119~136 (2016)
- Sorrell JM, "Fibroblast heterogeneity : more than skin deep", J Cell Sci, 117, pp.667~675 (2004)
- Driskell RR, "Distinct fibroblast lineages determine dermal architecture in skin development and repair", Nature, 504, pp.277~281 (2013)
- Imokawa, G., "Mechanism of UVB-induced wrinkling of the skin : Paracrine cytokine linkage between keratinocytes and fibroblasts leading to the stimulation of elastase", J Investig Dermatol Symp Proc, 14, pp.36~43 (2009)
- Imokawa, G., "Biological mechanisms underlying the ultraviolet radiation-induced formation of skin wrinkling and sagging I : Reduced skin elasticity, highly associated with enhanced dermal elastase activity, triggers wrinkling and sagging", Int J Mol Sci, 16, pp.7753~7775 (2015)
- Bossi O, "UV irradiation increases ROS production via PKCdelta signaling in primary murine fibroblasts", J Cell Biochem, 105, pp.194~207 (2008)
- Uemura M, "Novel 5alpha-steroid reductase(SRD5A3, type-3)is overexpressed in hormone-refractory prostate cancer", Cancer Sci, 99, pp.81~86 (2008)
- Chen W, "The 5α-reductase system and its inhibitors", Dermatology, 193, pp.177~184 (1996)
- Bayne E, "Immunohistochemical localization of types 1 and 2 5-reductase in human scalp", Br J Dermatol, 141, pp.481~491 (1999)
- The Pharmacopoeia Commission of PRC, "Pharmacopoeia of the People’s Republic of China Version 2000" (2000), Chemical Industry Press
- Luo Q, "Herbalogical textual research on"Gegen"", Zhongguo Zhongyao Zazhi, 32, pp.1141~1144 (2007)
- Kim IR, "Boncho-Hak", pp.184~185 (2007), Young-Lim Press
- Yun YG, "Oriental prescription & Explanation", pp.241~242 (2004), Euiseongdang Publishing
- 박설아, "인체 모유두세포의 증식에 미치는 갈근 에탄올추출물의 효과", 동의생리병리학회지, 31(3), pp.167~172 (2017), 한의병리학회[DOI]
- Adlercreutz H, "Phyto-oestrogens and cancer", The Lancet Oncology, 3, pp.364~373 (2002)
- Molavi O, "Silibinin sensitizes chemo-resistant breast cancer cells to chemotherapy", Pharm Biol, 55, pp.729~739 (2017)
- Whelchel A, "Inhibition of ERK activation attenuates endothelin-stimulated airway smooth muscle cell proliferation", Am J Respir Cell Mol Biol, 16, pp.589~596 (1997)
- Mella JM, "Efficacy and safety of finasteride therapy for androgenetic alopecia : a systematic review", Arch Dermatol, 146, pp.1141~1150 (2010)
- S Lee, "Adverse Sexual Effects of Treatment with Finasteride or Dutasteride for Male Androgenetic Alopecia: A Systematic Review and Meta-analysis", Acta Dermato Venereologica, pp.0~ (2018), Acta Dermato-Venereologica[DOI]
- Azzouni F, "The 5 alpha-reductase I isozyme family : A review of basic biology and their role in human diseases", Adv Urol (2011)[DOI]
- Jain R, "Avicequinone C isolated from Avicennia marina exhibits 5α-reductase-Type I inhibitory activity using an androgenic alopecia relevant cell-based assay system", Molecules, 19, pp.6809~6821 (2014)
- Gupta AK, "The efficacy and safety of 5α-reductase inhibitors in androgenetic alopecia : a network meta-analysis and benefit–risk assessment of finasteride and dutasteride", J Dermatolog Treat, 25, pp.156~161 (2014)
- Whelchel A, "Inhibition of ERK activation attenuates endothelin-stimulated airway smooth muscle cell proliferation", Am J Respir Cell Mol Biol, 16, pp.589~596 (1997)
- Molavi O, "Silibinin sensitizes chemo-resistant breast cancer cells to chemotherapy", Pharm Biol, 55, pp.729~739 (2017)