Diclofenac에 의한 백서의 장손상 모델에서 젖소초유의 효과
Effects of bovine colostrum in diclofenac induced gut injured rat model
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목적:비스테로이드성 항염제는 만성관절염, 통증, 발열 등에 가장 흔히 사용되는 약물이지만 소장을 포함한 전 위장관에 걸쳐 손상을 유발하여 출혈이나 단백소실 등이 발생한다. 비스테로이드성 항염제에 의한 소장손상을 유발하는 인자로는 소장 상피의 장벽 장애와 장내세균의 과증식 등이 제시되어 왔으며 초유는 출산 후 처음으로 얻어지는 우유로 각종 성장인자들과 면역글로불린 및 항균성 펩티드들이 풍부하게 함유되어 있다. 본 연구에서는 실험동물에서 지방질을 제거한 젖소초유의 diclofenac이 유발한 소장손상에 대한 영향을 알아보기로 하였다. 방법:백서 64마리를 대조군, diclofenac 단독 투여군, diclofenac 및 5%와 10% 젖소초유 동시 투여군으로 분류하여 젖소초유를 투여하는 군은 diclofenac 투여 5일 전부터 5%와 10% 젖소초유를 투여하였다. Diclofenac은 50 mg/kg를 피하투여 하였으며 이후 장투과성을 측정하였고, 장내 호기성균주수, 혈청 총단백과 알부민 수치 및 회장의 조직학적 소견을 관찰하였다. 결과:Diclofenac 투여에 의하여 백서에서 장투과성 증가 및 장내 세균의 과증식, 혈청 총단백과 알부민의 감소, 소장의 점막 손상 등이 유발되었다. 젖소초유의 투여에 의하여 장투과성의 증가 및 장내세균의 과증식이 감소되었으며 혈청 총단백과 알부민의 감소와 소장의 점막 손상이 개선되었다. 결론:젖소초유는 비스테로이드성 항염제에 의하여 유발된 소장병증과 장내세균전위의 억제에 유용할 것으로 사료된다.
Abstract
Background:Non-steroidal anti-inflammatory drugs (NSAIDs) are commonly prescribed medicine but induce damage throughout the entire gastrointestinal tract including small intestine with protein and blood loss. Impaired epithelial barrier function, overgrowth of luminal bacteria and others have been implicated in the pathogenesis of NSAID induced enteropathy. Colostrum is a first milk produced after birth and is particularly rich in growth factors, immunoglobulins and antimicrobial peptides. The present study aimed to exam whether defatted bovine colostrum reduce small intestinal injury caused by diclofenac in the animals. Methods:64 rats were utilized in four groups; control group, diclofenac group, diclofenac with 5% colostrum group and diclofenac with 10% colostrum group. The animals with colostrum were fed with 5% or 10% colostral solution for 5 days before diclofenac administration. Small intestinal injury was induced by administering a single dose of diclofenac (50 mg/kg subcutaneously). Epithelial permeability, enteric aerobic bacterial counts, serum albumin and protein levels, and pathologic findings of distal ileum were measured. Results:Diclofenac caused marked increase in intestinal permeability, enteric bacterial numbers and intestinal villous damage, and declines in serum levels of total protein and albumin. Co-administration of bovine colostrum reduced intestinal permeability and enteric bacterial numbers, declines in serum albumin and protein levels, and mucosal damage of small intestine induced by diclofenac. Conclusion:Bovine colostrums may have beneficial effects on preventing NSAID induced small intestinal injury and bacterial translocation.
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