IL-10 is Predominantly Produced by CD19(low)CD5(+) Regulatory B Cell Subpopulation: Characterisation of CD19 (high) and CD19(low) Subpopulations of CD5(+) B cells
0
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
IL-10 production by CD19(+)CD5(+) B cells was investigated, by determining the expression levels of CD19, a classical B cell marker. Peripheral mononuclear cells were stained with fluorescence-conjugated anti-CD5, anti-CD19, anti-IL-10, and Annexin V. Interestingly, IL-10-producing B cells were found to be localised within the CD19(low)CD5(+) B cell subset. Apoptotic changes were also observed mainly in CD19(low) cells among B cells. Thus, CD5(+) B cells should be classified as CD19(high) and CD19(low) cells, and the immunological significance of CD19 for the IL-10 production by CD5(+) B cells requires further studies.
Abstract
IL-10 production by CD19(+)CD5(+) B cells was investigated, by determining the expression levels of CD19, a classical B cell marker. Peripheral mononuclear cells were stained with fluorescence-conjugated anti-CD5, anti-CD19, anti-IL-10, and Annexin V. Interestingly, IL-10-producing B cells were found to be localised within the CD19(low)CD5(+) B cell subset. Apoptotic changes were also observed mainly in CD19(low) cells among B cells. Thus, CD5(+) B cells should be classified as CD19(high) and CD19(low) cells, and the immunological significance of CD19 for the IL-10 production by CD5(+) B cells requires further studies.
참고문헌 (19)
- Mizoguchi A, "A case for regulatory B cells", J Immunol, 176, pp.705~710 (2006)
- Gieni RS, "Ly1- (CD5-) B cells produce interleukin (IL)-10", Cell Immunol, 175, pp.164~170 (1997)
- Lee JH, "Allergen-specific B cell subset responses in cow's milk allergy of late eczematous reactions in atopic dermatitis", Cellular Immunology, 262(1), pp.44~51 (2010), Academic Press Inc.
- Yanaba K, "The development and function of regulatory B cells expressing IL-10 (B10 cells) requires antigen receptor diversity and TLR signals", J Immunol, 182, pp.7459~7472 (2009)
- Fujimoto M, "B cell signaling and autoimmune diseases: CD19", CD22 loop as a B cell signaling device to regulate the balance of autoimmunity, 2007, pp.46, 1~9
- Tedder TF, "Isolation of cDNAs encoding the CD19 antigen of human and mouse B lymphocytes. A new member of the immunoglobulin superfamily", J Immunol, 143, pp.712~717 (1989)
- Matsushita T, "Inhibitory role of CD19 in the progression of experimental autoimmune encephalomyelitis by regulating cytokine response", Am J Pathol, 168, pp.812~821 (2006)
- Bouaziz JD, "IL-10 produced by activated human B cells regulates CD4(+) T-cell activation in vitro", Eur J Immunol, 40, pp.2686~2691 (2010)
- Mauri C, "The ‘short’ history of regulatory B cells", Trends Immunol, 29, pp.34~40 (2008)
- Noh J, "Characterisation of allergen-specific responses of IL-10-producing regulatory B cells (Br1) in Cow Milk Allergy", CELLULAR IMMUNOLOGY, 264(2), pp.143~149 (2010), ACADEMIC PRESS INC ELSEVIER SCIENCE
- Taylor DK, "Loss of tolerance of anti-dsDNA B cells in mice overexpressing CD19", Mol Immunol, 43, pp.1776~1790 (2006)
- Sato S, "CD19 expression levels regulate B lymphocyte development: human CD19 restores normal function in mice lacking endogenous CD19", J Immunol, 158, pp.4662~4669 (1997)
- Lee Y, "Complement component C3d-antigen complexes can either augment or inhibit B lymphocyte activation and humoral immunity in mice depending on the degree of CD21", CD19 complex engagement, 2005, pp.175, 8011~8023
- Sato S, "Regulation of B lymphocyte development and activation by the CD19", CD21, Leu 13 complex requires the cytoplasmic domain of CD19, pp.J Immunol, 1997[DOI]
- Shoham T, "The tetraspanin CD81 regulates the expression of CD19 during B cell development in a postendoplasmic reticulum compartment", J Immunol, 171, pp.4062~4072 (2003)
- Fujimoto M, "CD19 amplifies B lymphocyte signal transduction by regulating Src-family protein tyrosine kinase activation", J Immunol, 162, pp.7088~7094 (1999)
- DiLillo DJ, "B10 cells and regulatory B cells balance immune responses during inflammation, autoimmunity, and cancer", Ann N Y Acad Sci, 1183, pp.38~57 (2010)
- Garaud S, "IL-10 production by B cells expressing CD5 with the alternative exon 1B", Ann N Y Acad Sci, 1173, pp.280~285 (2009)
- Díaz-Alderete A, "IL-10 production in B cells is confined to CD154+ cells in patients with systemic lupus erythematosus", J Autoimmun, 23, pp.379~383 (2004)
