Differentially Expressed Proteins in Nitric Oxide-Stimulated NIH/3T3 Fibroblasts: Implications for Inhibiting Cancer Development
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Purpose: Recent evidence shows that nitric oxide (NO) may exhibit both pro-cancer and anti-cancer activities. The present study aimed to determine the differentially expressedproteins in NO-treated NIH/3T3 fibroblasts in order to investigate whether NO induces proteins with pro-cancer or anti-cancer effects. Materials and Methods:The cells were treated with 300 μM of an NO donor 3,3-bis-(aminoethyl)-1-hydroxy-2-oxo-1-triazene (NOC-18) for 12 h. The changed protein patterns, which were separated by two-dimensional electrophoresis using pH gradients of 4‒7, were conclusively identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis of the peptide digests. Results: Seventeendifferentially expressed proteins were identified in NOC-18-treated cells. Nine proteins [vinculin protein, keratin 19, ubiquitous tropomodulin, F-actin capping protein (α1 subunit), tropomyosin 3, 26S proteasome-associated pad1 homolog, T-complex protein 1 (ε subunit) NG-dimethylarginine dimethylaminohydrolase, and heat shock protein 90] were increased and eight proteins (heat shock protein 70, glucosidaseII, lamin B1, calreticulin, nucleophosmin 1, microtubule-associated protein retinitis pigmentosa/end binding family member 1, 150 kD oxygen-regulated protein precursor, and heat shock 70-related protein albino or pale green 2) were decreased by NOC-18 in the cells. Thirteen proteins are related to the suppression of cancer cell proliferation, invasion, and metastasis while two proteins (heat shock protein 90 and NG-dimethylarginine dimethylaminohydrolase) are related to carcinogenesis. The functions of 150 kD oxygen-regulated protein precursor and T-complex protein 1 (ε subunit) are unknown in relation to carcinogenesis. Conclusion: Most proteins differentiallyexpressed by NOC-18 are involved in inhibiting cancer development.
Abstract
Purpose: Recent evidence shows that nitric oxide (NO) may exhibit both pro-cancer and anti-cancer activities. The present study aimed to determine the differentially expressedproteins in NO-treated NIH/3T3 fibroblasts in order to investigate whether NO induces proteins with pro-cancer or anti-cancer effects. Materials and Methods:The cells were treated with 300 μM of an NO donor 3,3-bis-(aminoethyl)-1-hydroxy-2-oxo-1-triazene (NOC-18) for 12 h. The changed protein patterns, which were separated by two-dimensional electrophoresis using pH gradients of 4‒7, were conclusively identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis of the peptide digests. Results: Seventeendifferentially expressed proteins were identified in NOC-18-treated cells. Nine proteins [vinculin protein, keratin 19, ubiquitous tropomodulin, F-actin capping protein (α1 subunit), tropomyosin 3, 26S proteasome-associated pad1 homolog, T-complex protein 1 (ε subunit) NG-dimethylarginine dimethylaminohydrolase, and heat shock protein 90] were increased and eight proteins (heat shock protein 70, glucosidaseII, lamin B1, calreticulin, nucleophosmin 1, microtubule-associated protein retinitis pigmentosa/end binding family member 1, 150 kD oxygen-regulated protein precursor, and heat shock 70-related protein albino or pale green 2) were decreased by NOC-18 in the cells. Thirteen proteins are related to the suppression of cancer cell proliferation, invasion, and metastasis while two proteins (heat shock protein 90 and NG-dimethylarginine dimethylaminohydrolase) are related to carcinogenesis. The functions of 150 kD oxygen-regulated protein precursor and T-complex protein 1 (ε subunit) are unknown in relation to carcinogenesis. Conclusion: Most proteins differentiallyexpressed by NOC-18 are involved in inhibiting cancer development.
참고문헌 (46)
- Moncada S, "Nitric oxide: physiology, pathophysiology, and pharmacology", Pharmacol Rev, 43, pp.109~142 (1991)
- Beck KF, "Inducible NO synthase: role in cellular signalling", J Exp Biol, 202(Pt 6), pp.645~653 (1999)
- Kanner J, "Nitric oxide as an antioxidant", Arch Biochem Biophys, 289, pp.130~136 (1991)
- Lechner M, "Inducible nitric oxide synthase (iNOS) in tumor biology: the two sides of the same coin", Semin Cancer Biol, 15, pp.277~289 (2005)
- Ying L, "An emerging role for endothelial nitric oxide synthase in chronic inflammation and cancer", Cancer Res, 67, pp.1407~1410 (2007)
- Sugita H, "Nitric oxide inhibits the proliferation and invasion of pancreatic cancer cells through degradation of insulin receptor substrate-1 protein", Mol Cancer Res, 8, pp.1152~1163 (2010)
- Carneiro ZA, "Nitric oxide photorelease from a trinuclear ruthenium nitrosyl complex and its in vitro cytotoxicity against melanoma cells", J Inorg Biochem, 134, pp.36~38 (2014)
- Brantley EC, "Nitric oxide-mediated tumoricidal activity of murine microglial cells", Transl Oncol, 3, pp.380~388 (2010)
- Patel HJ, "Constitutive expressions of type I NOS in human airway smooth muscle cells: evidence for an antiproliferative role", FASEB J, 13, pp.1810~1816 (1999)
- Seo JY, "Nitric oxide-induced IL-8 expression is mediated by NF-kappaB and AP-1 in gastric epithelial AGS cells", J Physiol Pharmacol, 60(Suppl 7), pp.101~106 (2009)
- Lim JW, "NF-kappaB, inducible nitric oxide synthase and apoptosis by Helicobacter pylori infection", Free Radic Biol Med, 31, pp.355~366 (2001)
- Davies CM, "Reactive nitrogen and oxygen species in interleukin-1-mediated DNA damage associated with osteoarthritis", Osteoarthritis Cartilage, 16, pp.624~630 (2008)
- Yu JH, "Proteome analysis of rat pancreatic acinar cells: implication for cerulein-induced acute pancreatitis", Proteomics, 3, pp.2446~2453 (2003)
- Faure O, "Inducible Hsp70 as target of anticancer immunotherapy: Identification of HLA-A*0201-restricted epitopes", Int J Cancer, 108, pp.863~870 (2004)
- Gabai VL, "Heat shock protein Hsp72 controls oncogene-induced senescence pathways in cancer cells", Mol Cell Biol, 29, pp.559~569 (2009)
- Zhao Q, "A mitochondrial specific stress response in mammalian cells", EMBO J, 21, pp.4411~4419 (2002)
- Sidera K, "A critical role for HSP90 in cancer cell invasion involves interaction with the extracellular domain of HER-2", J Biol Chem, 283, pp.2031~2041 (2008)
- Miyata Y, "The therapeutic target Hsp90 and cancer hallmarks", Curr Pharm Des, 19, pp.347~365 (2013)
- Ma L, "Dual targeting of heat shock proteins 90 and 70 promotes cell death and enhances the anticancer effect of chemotherapeutic agents in bladder cancer", Oncol Rep, 31, pp.2482~2492 (2014)
- Guttmann DM, "The heat shock proteins as targets for radiosensitization and chemosensitization in cancer", Cancer Biol Ther, 12, pp.1023~1031 (2011)
- Michalak M, "Ca2+ signaling and calcium binding chaperones of the endoplasmic reticulum", Cell Calcium, 32, pp.269~278 (2002)
- Hisaoka M, "Aberrant calreticulin expression is involved in the dedifferentiation of dedifferentiated liposarcoma", Am J Pathol, 180, pp.2076~2083 (2012)
- Pfister AS, "Amer2 protein interacts with EB1 protein and adenomatous polyposis coli (APC) and controls microtubule stability and cell migration", J Biol Chem, 287, pp.35333~35340 (2012)
- Tamura N, "Microtubule plus-ends within a mitotic cell are ‘moving platforms’ with anchoring, signalling and force-coupling roles", Open Biol, 2, pp.120132~ (2012)
- Morrison EE, "The APC-EB1 interaction", Adv Exp Med Biol, 656, pp.41~50 (2009)
- Kim MJ, "Depletion of end-binding protein 1(EB1)promotes apoptosis of human non-small-cell lung cancer cells via reactive oxygen species and Bax-mediated mitochondrial dysfunction", Cancer Lett, 339, pp.15~24 (2013)
- Sun S, "Circulating Lamin B1 (LMNB1) biomarker detects early stages of liver cancer in patients", J Proteome Res, 9, pp.70~78 (2010)
- Li L, "Lamin B1 is a novel therapeutic target of betulinic acid in pancreatic cancer", Clin Cancer Res, 19, pp.4651~4661 (2013)
- Suradej B, "Glucosidase II exhibits similarity to the p53 tumor suppressor in regards to structure and behavior in response to stress signals: a potential novel cancer biomarker", Oncol Rep, 30, pp.2511~2519 (2013)
- Wong JC, "Nucleophosmin 1, upregulated in adenomas and cancers of the colon, inhibits p53-mediated cellular senescence", Int J Cancer, 133, pp.1567~1577 (2013)
- Deng WH, "Effects of ORP150 on appearance and function of pancreatic beta cells following acute necrotizing pancreatitis", Pathol Res Pract, 207, pp.370~376 (2011)
- Li C, "Overexpression of Apg-2 increases cell proliferation and protects from oxidative damage in BaF3-BCR", ABL cells, 2010, pp.36, 899~904
- Gotoh K, "Apg-2 has a chaperone-like activity similar to Hsp110 and is overexpressed in hepatocellular carcinomas", FEBS Lett, 560, pp.19~24 (2004)
- Carisey A, "Vinculin, an adapter protein in control of cell adhesion signalling", Eur J Cell Biol, 90, pp.157~163 (2011)
- Goldmann WH, "Vinculin, cell mechanics and tumour cell invasion", Cell Biol Int, 37, pp.397~405 (2013)
- Kostyukova AS, "Tropomodulins and tropomodulin", tropomyosin interactions, 2008, pp.65, 563~569
- Franzén B, "Expression of tropomyosin isoforms in benign and malignant human breast lesions", Br J Cancer, 73, pp.909~913 (1996)
- Zheng Q, "Role of high-molecular weight tropomyosins in TGF-beta-mediated control of cell motility", Int J Cancer, 122, pp.78~90 (2008)
- 임보현, "Overexpression of nicotinamide N-methyltransferase in gastric cancer tissues and its potential post-translational modification", Experimental and Molecular Medicine, 38(5), pp.455~465 (2006), 생화학분자생물학회
- Lee YJ, "Prognostic value of CAPZA1 overexpression in gastric cancer", Int J Oncol, 42, pp.1569~1577 (2013)
- Schoenfeld A, "Keratin 19 mRNA measurement to detect micrometastases in lymph nodes in breast cancer patients", Br J Cancer, 74, pp.1639~1642 (1996)
- Ju JH, "Regulation of cell proliferation and migration by keratin19-induced nuclear import of early growth response-1 in breast cancer cells", Clin Cancer Res, 19, pp.4335~4346 (2013)
- Guenther MG, "Assembly of the SMRT-histone deacetylase 3 repression complex requires the TCP-1 ring complex", Genes Dev, 16, pp.3130~3135 (2002)
- Nikawa J, "A novel function of the human chaperonin CCT epsilon subunit in yeast", Biosci Biotechnol Biochem, 76, pp.199~201 (2012)
- Lagadec C, "Tumor cells with low proteasome subunit expression predict overall survival in head and neck cancer patients", BMC Cancer, 14, pp.152~ (2014)
- Boult JK, "Active site mutant dimethylarginine dimethylaminohydrolase 1 expression confers an intermediate tumour phenotype in C6 gliomas", J Pathol, 225, pp.344~352 (2011)