Protein Expression Profile using Two-Dimensional Gel Analysis in Squamous Cervical Cancer Patients
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Purpose: Screening in cervical cancer is now progressing to discover candidate genes and proteins that may serve as biological markers and that play a role in tumor progression. We examined the protein expression patterns of the squamous cell carcinoma (SCC) tissues from Korean women with using two- dimensional polyacrylamide gel electrophoresis (2-DE) and matrix assisted laser desorption/ionization-time of flight (MALDI- TOF) mass spectrometer. Materials and Methods: Normal cervix and SCC tissues were solubilized and 2-DE was performed using pH 3～10 linear IPG strips of 17 cm length. The protein expression was evaluated using PDQuest 2-D softwareTM. The differentially expressed protein spots were identified with a MALDI-TOF mass spectrometer, and the peptide mass spectra identifications were performed using the Mascot program and by searching the Swiss-prot or NCBInr databases. Results: A total of 35 proteins were detected in SCC. 17 proteins were up-regulated and 18 proteins weredownregulated. Among the proteins that were identified, 12 proteins (pigment epithelium derived factor, annexin A2 and A5, keratin 19 and 20, heat shock protein 27, smooth muscle protein 22 alpha, α-enolase, squamous cell carcinoma antigen 1 and 2, glutathione S-transferase and apolipoprotein a1) were protein previously known to be involved in tumor, and 21 proteins were newly identified in this study. Conclusion: 2-DE offers the total protein expression profiles of SCC tissues; further characterization of these differentially expressed proteins will give a chance to identify the badly needed tumor-specific diagnostic markers for SCC. (Cancer Res Treat. 2006;38:99-107)
Abstract
Purpose: Screening in cervical cancer is now progressingto discover candidate genes and proteins that mayserve as biological markers and that play a role in tumorprogression. We examined the protein expression patternsof the squamous cell carcinoma (SCC) tissues fromKorean women with using two- dimensional polyacrylamidegel electrophoresis (2-DE) and matrix assistedlaser desorption/ionization-time of flight (MALDI- TOF)mass spectrometer. Materials and Methods: Normal cervix and SCC tissueswere solubilized and 2-DE was performed using pH 3～10linear IPG strips of 17 cm length. The protein expressionwas evaluated using PDQuest 2-D softwareTM. The differentiallyexpressed protein spots were identified with aMALDI-TOF mass spectrometer, and the peptide massspectra identifications were performed using the Mascotprogram and by searching the Swiss-prot or NCBInrdatabases. Results: A total of 35 proteins were detected in SCC. 17 proteins were up-regulated and 18 proteins weredownregulated. Among the proteins that were identified, 12proteins (pigment epithelium derived factor, annexin A2and A5, keratin 19 and 20, heat shock protein 27, smoothmuscle protein 22 alpha, α-enolase, squamous cell carcinomaantigen 1 and 2, glutathione S-transferase andapolipoprotein a1) were protein previously known to beinvolved in tumor, and 21 proteins were newly identifiedin this study. Conclusion: 2-DE offers the total protein expressionprofiles of SCC tissues; further characterization of thesedifferentially expressed proteins will give a chance toidentify the badly needed tumor-specific diagnostic markersfor SCC. (Cancer Res Treat. 2006;38:99-107)
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