Determination of streptomycin in kiwifruit samples using LC-ESI-MS/MS
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In May 2012, a safety hazard issue arose because some kiwifruit growers in New Zealand had sprayed streptomycin to prevent kiwifruit canker. Therefore, for food safety management, analytical methods to determine streptomycin residues in kiwifruits are required. We developed an analytical method to determine streptomycin residues in kiwifruit samples using liquid chromatograph tandem mass spectrometer (LC-ESI-MS/MS). Streptomycin residues in samples were extracted using 1% formic acid in methanol, centrifugation for 10 min, and subsequent supernatant filtration. Purified samples were subjected to LC-ESI-MS/MS to confirm presence of and quantify streptomycin residues. Average streptomycin recoveries (6 replicates each sample) were in the range of 94.8%- 110.6% with relative standard deviations of <10%. The linearity of the concentration range of 0.01-5.0 mg/ kg using a matrix-matched calibration gave R2 = 0.9995. The limit of quantification (LOQ) was 0.01 mg/kg. Results showed that our analytical method is rapid, simple, and sensitive, with easy sample preparation.
Abstract
In May 2012, a safety hazard issue arose because some kiwifruit growers in New Zealand had sprayed streptomycin to prevent kiwifruit canker. Therefore, for food safety management, analytical methods to determine streptomycin residues in kiwifruits are required. We developed an analytical method to determine streptomycin residues in kiwifruit samples using liquid chromatograph tandem mass spectrometer (LC-ESI-MS/MS). Streptomycin residues in samples were extracted using 1% formic acid in methanol, centrifugation for 10 min, and subsequent supernatant filtration. Purified samples were subjected to LC-ESI-MS/MS to confirm presence of and quantify streptomycin residues. Average streptomycin recoveries (6 replicates each sample) were in the range of 94.8%- 110.6% with relative standard deviations of <10%. The linearity of the concentration range of 0.01-5.0 mg/ kg using a matrix-matched calibration gave R2 = 0.9995. The limit of quantification (LOQ) was 0.01 mg/kg. Results showed that our analytical method is rapid, simple, and sensitive, with easy sample preparation.
참고문헌 (10)
- L. L. Brunton, "The Pharmacological Basis of Therapeutics" (2005), McGraw Hill publication
- J. Kilcoyne, , J. Chromatogra. A, 1217, pp.7123~7130 (2010)
- P. Edder, , J. Chromatogra. A, 830, pp.345~351 (1999)
- "Environmental Protection Agency" (2014)[URL]
- I. P. Kaur, , Int. J. Phar, 1, pp.110~117 (2011)
- H. Kambara, , J. Antib, 35, pp.67~73 (1982)
- M. Mezcua, , J Chromatogra. A, 1109, pp.222~227 (2006)
- R. Sheridan, , J Agric. Food Chem, 56, pp.3509~3516 (2008)
- N. I. Stefan, , J. AOAC International, 82, pp.1017~1042 (1999)
- M. Bruijnsvoort, , J. Chromatogra. A, 1058, pp.137~142 (2004)