Simultaneous Analysis of Conazole Fungicides in Garlic by Q-TOF Mass Spectrometer Coupled with a Modified QuEChERS Method
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BACKGROUND:The conazoles, difenoconazole, diniconazole, hexaconazole, penconazole and tetraconazole are a large class of synthetic fungicides used extensively for foliage and seed treatments in agricultural crops. The extensive use of conazoles has brought concerns on the potentiality of environmental contamination and toxicity. Thus studies on the development of methods for monitoring the conazoles are required. METHODS AND RESULTS: A modified quick, easy, effective, rugged and safe (QuEChERS) method was involved in sample preparation. Quadrapole time of flight mass spectrometer (Q-TOF MS) in electron spray ionization (ESI) mode was employed to determine conazoles in garlic samples. The limit of detection (LOD) and limit of quantification (LOQ) of conazoles by Q-TOF-MS ranged from 0.001 to 0.002 mg/L and 0.002 to 0.005 mg/L, respectively. Q-TOF-MS analysis exhibited less than 2.6 ppm error of accurate mass measurements for the detection of conazoles spiked at 0.05 mg/L in garlic matrix. Recovery values of conazoles fortified in garlic samples at 0.02, 0.05 and 0.1 mg/L were between 79.2 and 106.2% with a maximum 11.8% of standard deviation. No detectable conazoles were found in the domestic market samples by using the Q-TOF-MS method. CONCLUSION(s):High degree of confirmation for conazoles by accurate mass measurements demonstrated that Q-TOF-MS analysis combined with a QuEChERS method may be applicable to simultaneous determination of conazoles in garlic samples.
Abstract
BACKGROUND:The conazoles, difenoconazole, diniconazole, hexaconazole, penconazole and tetraconazole are a large class of synthetic fungicides used extensively for foliage and seed treatments in agricultural crops. The extensive use of conazoles has brought concerns on the potentiality of environmental contamination and toxicity. Thus studies on the development of methods for monitoring the conazoles are required. METHODS AND RESULTS: A modified quick, easy, effective, rugged and safe (QuEChERS) method was involved in sample preparation. Quadrapole time of flight mass spectrometer (Q-TOF MS) in electron spray ionization (ESI) mode was employed to determine conazoles in garlic samples. The limit of detection (LOD) and limit of quantification (LOQ) of conazoles by Q-TOF-MS ranged from 0.001 to 0.002 mg/L and 0.002 to 0.005 mg/L, respectively. Q-TOF-MS analysis exhibited less than 2.6 ppm error of accurate mass measurements for the detection of conazoles spiked at 0.05 mg/L in garlic matrix. Recovery values of conazoles fortified in garlic samples at 0.02, 0.05 and 0.1 mg/L were between 79.2 and 106.2% with a maximum 11.8% of standard deviation. No detectable conazoles were found in the domestic market samples by using the Q-TOF-MS method. CONCLUSION(s):High degree of confirmation for conazoles by accurate mass measurements demonstrated that Q-TOF-MS analysis combined with a QuEChERS method may be applicable to simultaneous determination of conazoles in garlic samples.
참고문헌 (24)
- Anastassiades, M., "Fast and easy multiresidue method employing acetonitrile extraction", partitioning and “dispersive solid-phase extraction” for the determination of pesticide residues in produce, 2003, pp.86, 412~431
- Borek, C, "Recent advances on the nutritional effects associated with the use of garlic as a supplement", J. Nutr, 131, pp.1010~1015 (2001)
- Darouiche, R.O., "Potential Use of a Simplified Method for Determination of itraconazole levels in plasma and esophageal tissue by using high-performance liquid chromatography", J. Antimicrob, 757, pp.757~759 (1995)
- Ferrer, I., "Multi-residue method for the analysis of 101 pesticides and their degradates in food and water samples by liquid chromatography", time-of-flight mass spectrometry, 2007, pp.1175, 24~37
- Gilbert-López, B., "Sample treatment and determination of pesticide residues in fatty vegetable matrices", Talanta, 79, pp.109~128 (2009)
- Gilbert-López, B., "Multi-residue determination of pesticides in fruit-based soft drinks by fast liquid chromatography time-of-flight mass spectrometry", Talanta, 81, pp.1310~1321 (2010)
- Harris, S.C., "Assay of fluconazole by megabore capillary gas-liquid chromatography with nitrogen selective detection, Antimicrob", Agents Chemother, 33, pp.714~716 (1989)
- Kjærsta, M.B., "Endocrine disrupting effect in vitro of conazole antifungals used as pesticides and pharmaceuticals", Reprod.Toxico, 30, pp.573~582 (2010)
- Konwick, B.J., "Bioaccumulation and biotransformation of chiral triazole fungicides in rainbow trout (Oncorhynchusmykiss)", Aquat.Toxicol, 80, pp.372~381 (2006)
- Lacina, O., "Identification", quantification of multiple pesticide residues in food plants by mass spectrometry, 2010, pp.1217, 648~659
- Lehotay, S., "Comparison of QuEChERS sample preparation methods for the analysis of pesticide residues in fruit and vegetables", J. Chromatogr. A, 1217, pp.2548~2560 (2010)
- Pareja, L., "Trace analysis of pesticides in paddy field water by direct injection using liquid chromatography-quadrupole-linear ion trap-mass spectrometry", J. Chromatogr. A, 1218, pp.4790~4798 (2011)
- Petrovic, M., "Application of liquid chromatography", quadrupole time-of-flight mass spectrometry (LC-QqTOF-MS) in the environmental analysis, 2006, pp.41, 1259~1267
- Rege, A.B, "Rapid and sensitive assay for fluconazole which uses gas chromatography with electron capture detection", Antimicrorob. Agents Chemother, pp.647~650 (1992)
- Ren, L., "Characterization of the in vivo and in vitro metabolic profile of PAC-1 using liquid chromatography-mass spectrometry", J. Chromatogr. B, 876, pp.47~53 (2008)
- Ross, J.C, "In vivo mutagenicity of conazole fungicides correlates with tumorigenicity", Mutat. Res, 24, pp.149~152 (2009)
- Stevinson, C., "Garlic for Treating Hypercholesterolemia", Ann. Int. Med, 133, pp.420~429 (2000)
- Stroh, J.G, "Automated Sub-ppm mass accuracy on an EST-TOF for use with drug discovery compound librarise", J. Am. Soc. Mass spectrum, 18, pp.161~1616 (2007)
- Tully, D.B., "Gene expression profiling in liver and testis of rats to characterize the toxicity of triazole fungicides", Toxicol. Appl. Pharmacol, 30, pp.573~582 (2006)
- Warnock, D.W., "Comparison of high performance liquid chromatographic and microbiological methods for determination of itraconazole", J. Antimicrob, 21, pp.93~100 (1988)
- Wilkowska, A, "Determination of pesticide residues in food matrices using the QuEChERS methodology", Food Chem, 123, pp.803~812 (2011)
- Woestenborghs, R., "Determination of itraconazole in plasm and animal tissues by high-performance liquid chromatography", J. Chromatogr, 413, pp.332~337 (1987)
- Wolff, J.C., "Accurate mass liquid chromatography", mass spectrometry on quadrupole orthogonal acceleration time-of-flight mass analyzers using switching between separate sample and reference sprays, 2001, pp.73, 2605~
- Wood, P. R., "Gas chromatographic method for the determination of fluconazole, a novel antifungal agent, in human plasma and urine", J. Chromatogr, 383, pp.179~186 (1986)