三神丸 에탄올 추출물(SSH)의 MRSA에 대한 항균활성 연구
A study on Antibacterial Activity of Samsinhwan Ethanol Extract against MRSA
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초록
이번 연구의 목적은 MRSA에 대하여 三神丸의 항균효과와 기존 항생제인 Oxacillin과 Ciprofloxacin과의 synergy effect를 확인하고 그 기전을 밝히는 것이다. 이에 三神丸의 Disc 확산 실험 및 최소억제농도(MIC)를 측정하여 MARA에 대한 항균 활성 및 활성농도를 검증하고, 항생제와 三神丸 에탄올 추출물을 병행 처리하여 병용 효과를 확인하고, 시간에 따른 생장 억제 효과를 확인하는 절차와 방법을 통해 三神丸이 MARA에 대한 항균 활성으로 oxacillin 또는 ciprofloxacin과 결합 되었을 때, 시너지 및 부분 시너지 효과를 보임으로써 in vitro에서 우수한 항균 물질임이 연구 결과로 나타났음을 시사하는 바이다. 아울러 MARA 내성의 제어를 위한 신약 개발에 본 연구가 의미 있는 자료가 되기를 기대할 뿐 아니라, 내성 균주를 극복하기 위한 항생물질의 연구와 개발에 더욱 박차를 가하는 계기가 되기를 바란다.
Abstract
The purpose of this study is to identify the antimicrobial effects of SSH(三神丸) and synerggy effects of the existing antibiotics Oxacillin and Ciprofloxacin on MRSA and to identify the mechanisms. In this case, the procedure and method for verifying anti-bacterial activity and active concentration of MARA by measuring the minimum inhibitory concentration (MIC) of the trichin is used to verify the potency and active concentration of MARA, to confirm the potency of the disease by treating antibiotics and trichine ethanol extract in parallel, and to confirm the anti-bacterial effect over time, when the trichine is activated as an anti-bacterial activity to MARA, and a compound. In addition, we hope that this research will not only serve as meaningful data for the development of new drugs to control MARA immunity, but also serve as an opportunity to further accelerate the research and development of antibiotics to overcome resistant strains.
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