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Genotype analysis of Cryptosporidium spp. Prevalent in a rural village in Hwasun-gun, Republic of Korea

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Abstract

Two species of Cryptosporidiumare known to infect man; C. hominis which shows anthroponotic trans-mission between humans, and C. parvumwhich shows zoonotic transmission between animals or between animalsand man. In this study, we focused on identifying genotypes of Cryptosporidiumprevalent among inhabitants anddomestic animals (cattle and goats), to elucidate transmittal routes in a known endemic area in Hwasun-gun,Jeollanam-do, Republic of Korea. The existence of Cryptosporidiumoocysts was confirmed using a modified Ziehl-Neelsen stain. Human infections were found in 7 (25.9%) of 27 people examined. Cattle cryptosporidiosis cases con-formed on the small subunit of the rRNA gene using both PCR-RFLP and sequence analysis. Most of the human iso-lates were mixtures of C. hominisand C. parvumgenotypes and similar PCR-RFLP patterns were observed in cattleand goat isolates. However, sequence analyses identified only C. hominisin all isolates examined. The natural infec-tion of cattle and goats with C. hominiscryptosporidiosis in the studied area is caused by mixtures of C. hominisand C. parvumoocysts originating from bothinhabitants and domestic animals.

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관련 논문AI추천

Prevalence of cryptosporidiosis among the villagers and domestic animals in several rural areas of Korea서민, 허선, 김동수, 김석일, 손운목, 유재란, 이규재, 최해연대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2004 · 제42권 제1호Genotype and animal infectivity of a human isolate of Cryptosporidium parvum in the Republic of Korea (Brief Communication)국상미, 박순정, 박재환, 용태순, 채종일대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2004 · 제42권 제2호Prevalenc of diarrhea caused by Cryptosporidium parvum in non-HIV patients in Jeollanam-do, Korea송현제, 유재란, 이종규대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2005 · 제43권 제3호A survey of cryptosporidiosis among 2,541 residents of 25 coastal islands in Jeollanam-do (Province), Republic of Korea국상미, 김재립, 김효진, 박재환, 신은희, 이순형, 임한종, 채종일대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2006 · 제44권 제4호Molecular Prevalence and Genotypes of Cryptosporidium parvum and Giardia duodenalis in Patients with Acute Diarrhea in Korea, 2013-2016이명노, 조신형, Da-Won Ma, Sang-Eun Lee, Sung-Hee Hong대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2019 · 제57권 제5호전북 지역의 Cryptosporidium oocyst 분포에 대한 역학적 조사김유현대한임상검사과학회 · 대한임상검사과학회지1997 · 제29권 제1호

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