In Vitro Maintenance of Clonorchis sinensis Adult Worms
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Clonorchis sinensis is a biological carcinogen inducing human cholangiocarcinoma, and clonorchiasis is one of the important endemic infectious diseases in East Asia. The present study investigated survival longevity of C. sinensis adult worms in various in vitro conditions to find the best way of keeping the worms longer. The worms were maintained in 0.85% NaCl, 1×PBS, 1×Locke’s solution, RPMI-1640, DMEM, and IMDM media, and in 1×Locke’s solution with different supplements. All of the worms died within 3 and 7 days in 0.85% NaCl and 1×PBS, respectively, but survived up to 57 days in 1×Locke’s solution. The worms lived for 106 days in DMEM, and 114 days in both RPMI-1640 and IMDM media. The survival rate in RPMI-1640 medium was the highest (50%) compared to that in DMEM (20±10%) and in IMDM (33.3±25.2%) after 3 months. The 1×Locke’s solution with 0.005% bovine bile supplement showed increased duration of maximum survival from 42 days to 70 days. Higher concentration of bile supplements than 0.005% or addition of glucose were disadvantageous for the worm survival. The worms died rapidly in solutions containing L-aspartic acid, L-glutamic acid, and adenine compared to L-arginine, L-serine, and L-tryptophan. In conclusion, the 1×Locke’s solution best supports the worms alive among inorganic solutions for 57 days, and the RPMI-1640 medium maintains living C. sinensis adults better and longer up to 114 days in vitro than other media.
Abstract
Clonorchis sinensis is a biological carcinogen inducing human cholangiocarcinoma, and clonorchiasis is one of the important endemic infectious diseases in East Asia. The present study investigated survival longevity of C. sinensis adult worms in various in vitro conditions to find the best way of keeping the worms longer. The worms were maintained in 0.85% NaCl, 1×PBS, 1×Locke’s solution, RPMI-1640, DMEM, and IMDM media, and in 1×Locke’s solution with different supplements. All of the worms died within 3 and 7 days in 0.85% NaCl and 1×PBS, respectively, but survived up to 57 days in 1×Locke’s solution. The worms lived for 106 days in DMEM, and 114 days in both RPMI-1640 and IMDM media. The survival rate in RPMI-1640 medium was the highest (50%) compared to that in DMEM (20±10%) and in IMDM (33.3±25.2%) after 3 months. The 1×Locke’s solution with 0.005% bovine bile supplement showed increased duration of maximum survival from 42 days to 70 days. Higher concentration of bile supplements than 0.005% or addition of glucose were disadvantageous for the worm survival. The worms died rapidly in solutions containing L-aspartic acid, L-glutamic acid, and adenine compared to L-arginine, L-serine, and L-tryptophan. In conclusion, the 1×Locke’s solution best supports the worms alive among inorganic solutions for 57 days, and the RPMI-1640 medium maintains living C. sinensis adults better and longer up to 114 days in vitro than other media.
참고문헌 (29)
- Bouvard V, "A review of human carcinogens-part B: biological agents", Lancet Oncol, 10, pp.321~322 (2009)
- 신해림, "Descriptive Epidemiology of Cholangiocarcinoma and Clonorchiasis in Korea", Journal of Korean Medical Science, 25(7), pp.1011~1016 (2010), 대한의학회
- Hong ST, "Clonorchis sinensis and clonorchiasis, an update", Parasitol Int, 61, pp.17~24 (2012)
- Kim YJ, "Resistance of cholangiocarcinoma cells to parthenolide-induced apoptosis by the excretory- secretory products of Clonorchis sinensis", Parasitol Res, 104, pp.1011~1016 (2009)
- Kim JH, "Correlation between discharged worms and fecal egg counts in human clonorchiasis", PLoS Negl Trop Dis, 5, pp.e1339~ (2011)
- Uddin MH, "Strain variation in the susceptibility and immune response to Clonorchis sinensis infection in mice", PARASITOLOGY INTERNATIONAL, 61, pp.118~123 (2012), ELSEVIER IRELAND LTD
- Rahman SMM, "Coproantigen capture ELISA for detection of Clonorchis sinensis infection in experimentally infected rats", Parasitol Int, 61, pp.203~207 (2012)
- Liang C, "Experimental establishment of life cycle of Clonorchis sinensis", Chinese J Parasitol Parasit Dis, 27, pp.148~150 (2009)
- Kim YJ, "Proliferative effect of excretory", secretory products from Clonorchis sinensis on the human epithelial cell line HEK293 via regulation of the transcription factor E2F1, 2008, pp.102, 411~417
- "Differential gene expression profiling in human cholangiocarcinoma cells treated with Clonorchis sinensis excretory-secretory products", PARASITOLOGY RESEARCH, 104, pp.1035~1046 (2009), SPRINGER
- "Identification of a serodiagnostic antigen, legumain, by immunoproteomic analysis of excretory-secretory products of Clonorchis sinensis adult worms", PROTEOMICS, 9(0), pp.3066~3078 (2009), WILEY-V C H VERLAG GMBH
- "A family of cathepsin F cysteine proteases of Clonorchis sinensis is the major secreted proteins that are expressed in the intestine of the parasite", MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 170, pp.7~16 (2010), ELSEVIER SCIENCE BV
- "Bile components and amino acids affect survival of the newly excysted juvenile Clonorchis sinensis in maintaining media", PARASITOLOGY RESEARCH, 103, pp.1019~1024 (2008), SPRINGER
- Russo MA, "Effects of medium calcium, and agents affecting cytoskeletal function, on cellular volume and morphology in liver tissue in vitro", J Cell Biochem, 113, pp.1915~1925 (2012)
- Shemarova IV, "Signal function of calcium-binding proteins in lower eukaryotes", Tsitologiia, 53, pp.600~614 (2011)
- Shunyu Li, "Organ-specific antigens of Clonorchis sinensis", The Korean Journal of Parasitology, 42(4), pp.169~174 (2004), 대한기생충학회
- Fredensborg BL, "In vitro cultivation of Maritrema novaezealandensis(Microphallidae): the effect of culture medium on excystation, survival and egg production", Parasitol Res, 95, pp.310~313 (2005)
- Fried B, "Effects of tonicity, digestive enzymes and bile salts, and nutrient media on the survival of excysted metacercariae of Echinostoma caproni", Parasitol Res, 93, pp.1~4 (2004)
- Huesca-Guillén A, "Paramphistomum spp: improved artificial excystment and in vitro culture of immature and adult stages", Parasitol Res, 102, pp.41~45 (2007)
- Mayra Grano-Maldonado, "In Vitro Cultivation of Cymatocarpus solearis (Brachycoeliidae) Metacercariae to Obtain the Adult Stage without the Marine Turtle Definitive Host", The Korean Journal of Parasitology, 48(1), pp.49~55 (2010), 대한기생충학회
- Tsai SJ, "Determination of bile acids in pig liver, pig kidney and bovine liver by gas chromatography-chemical ionization tandem mass spectrometry with total ion chromatograms and extraction ion chromatograms", J Chromatogra, 1218, pp.524~533 (2011)
- Janowitz P, "Comparison of gall bladder bile and endoscopically obtained duodenal bile", Gut, 31, pp.1407~1410 (1990)
- Pedrini P, "Xanthomonas maltophilia CBS 897.97 as a source of new 7beta- and 7alpha-hydroxysteroid dehydrogenases and cholylglycine hydrolase: improved biotransformations of bile acids", Steroids, 71, pp.189~198 (2006)
- BernardFRIED, "Effects of exogenous glucose on survival and infectivity of Schistosoma mansoni cercariae", The Korean Journal of Parasitology, 40(1), pp.55~58 (2002), 대한기생충학회
- Ponder EL, "Effects of glucose on survival, infectivity and linear movement of the cercariae of Echinostoma caproni", J Helminthol, 78, pp.185~187 (2004)
- Schlotterer A, "C. elegans as model for the study of high glucose-mediated life span reduction", Diabetes, 58, pp.2450~2456 (2009)
- Lee SJ, "Glucose Shortens the Life Span of C. elegans by Downregulating DAF-16", FOXO Activity and Aquaporin Gene Expression, 2009, pp.10(5), 379~391[DOI]
- Schulz TJ, "Glucose restriction extends Caenorhabditis elegans life span by inducing mitochondrial respiration and increasing oxidative stress", Cell Metab, 6, pp.280~293 (2007)
- Golembewski EK, "The HSV-2 protein ICP 10PK prevents neuronal apoptosis and loss of function in an in vivo model of neurodegeneration associated with glutamate", Exp Neurol, 203, pp.381~393 (2007)