Effect of Temperature on the Killing of Opisthorchis viverrini Eggs In Vitro
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Contaminated liver fluke egg in the environment has led to the high prevalence of human opisthorchiasis asso- ciated with cholangiocarcinoma in Southeast Asia. To find the effective lessening methods of Opisthorchis viverrini eggs in the contaminated environment, we investigated the temperature conditions for killing of these trematode eggs in vitro. Numerous O. viverrini eggs were obtained in the proximal part of uteri of adult worms from experimental hamsters. Ma- ture eggs with miracidium were allocated by experimental groups (2 control: positive and negative and 4 treatment: 50, 60, 70, and 80°C) with 0.85% saline, and treated by the experimental plan. Eggs in each experimental groups were ob- served under the confocal microscope after stain with Propidium Iodide (PI) to evaluate the effect of temperatures. Eggs in 70 and 80°C groups were all killed after over 10 min heated. Majority of eggs in 60°C (10, 15, and 30 min heated), 70 and 80°C (5 min heated) groups were inactivated. However in 50°C group, below half of eggs were to be killed in all time lapse (10, 15 and 30 min). In order to prevent O. viverrini infection and cholangiocarcinoma, direct treatment of sewage by heating at 70 or 80°C at least 10 min is essential. Therefore, treatment of O. viverrini eggs at a high temperature is a po- tential method for controlling egg contamination in sewage.
Abstract
Contaminated liver fluke egg in the environment has led to the high prevalence of human opisthorchiasis asso- ciated with cholangiocarcinoma in Southeast Asia. To find the effective lessening methods of Opisthorchis viverrini eggs in the contaminated environment, we investigated the temperature conditions for killing of these trematode eggs in vitro. Numerous O. viverrini eggs were obtained in the proximal part of uteri of adult worms from experimental hamsters. Ma- ture eggs with miracidium were allocated by experimental groups (2 control: positive and negative and 4 treatment: 50, 60, 70, and 80°C) with 0.85% saline, and treated by the experimental plan. Eggs in each experimental groups were ob- served under the confocal microscope after stain with Propidium Iodide (PI) to evaluate the effect of temperatures. Eggs in 70 and 80°C groups were all killed after over 10 min heated. Majority of eggs in 60°C (10, 15, and 30 min heated), 70 and 80°C (5 min heated) groups were inactivated. However in 50°C group, below half of eggs were to be killed in all time lapse (10, 15 and 30 min). In order to prevent O. viverrini infection and cholangiocarcinoma, direct treatment of sewage by heating at 70 or 80°C at least 10 min is essential. Therefore, treatment of O. viverrini eggs at a high temperature is a po- tential method for controlling egg contamination in sewage.
참고문헌 (33)
- Sripa B, "Food-borne trematodiases in Southeast Asia : epidemiology, pathology, clinical manifestation and control", Adv Parasitol, 72, pp.305~350 (2010)
- Sripa B, "Cholangiocarcinoma : lessons from Thailand", Curr Opin Gastroenterol, 24, pp.349~356 (2008)
- Control of foodborne trematode infections. Report of a WHO Study Group, "World Health Organization", World Health Organization, 1~157, pp.World Health Organ Tech Rep Ser 1995; 849 (1995)
- Kaewkes S, "Taxonomy and biology of liver flukes", Acta Trop, 88, pp.177~186 (2003)
- Jongsuksuntigul P, "Opisthorchiasis control in Thailand", Acta Trop, 88, pp.229~232 (2003)
- Kobayashi J, "An epidemiological study on Opisthorchis viverrini infection in Lao villages", Southeast Asian J Trop Med Public Health, 31, pp.128~132 (2000)
- Yossepowitch O, "Opisthorchiasis from imported raw fish", Emerg Infect Dis, 10, pp.2122~2126 (2004)
- Sithithaworn P, "Epidemiology of Opisthorchis viverrini", Acta Trop, 88, pp.187~194 (2003)
- Andrews RH, "Opisthorchis viverrini: an underestimated parasite in world health", Trends Parasitol, 24, pp.497~501 (2008)
- Fried B, "Food-borne intestinal trematodiases in humans", Parasitol Res, 93, pp.159~170 (2004)
- Ditrich O, "Occurrence of some medically important flukes(Trematoda : Opisthorchiidae and Heterophyidae)in Nam Ngum water reservoir, Laos", Southeast Asian J Trop Med Public Health, 21, pp.482~488 (1990)
- Scholtz T, "Helminth parasites of cats from the Vientiane Province, Laos, as indicators of the occurrence of causative agents of human parasitosis", Parasite, 10, pp.343~350 (2003)
- Giboda M, "Current status of food-borne parasitic zoonoses in Laos", Southeast Asian J Trop Med Public Health, 22(suppl), pp.56~61 (1991)
- Enes JE, "Prevalence of Opisthorchis viverrini infection in the canine and feline hosts in three villages, Khon Kaen Province, northeastern Thailand", Southeast Asian J Trop Med Public Health, 41, pp.36~ (2010)
- Alouini Z, "Destruction of helminth eggs by photosensitized porphyrin", J Environ Monit, 3, pp.548~551 (2001)
- Sengupta ME, "Use of Moringa oleifera seed extracts to reduce helminth egg numbers and turbidity in irrigation water", Water Res, 46, pp.3646~3656 (2012)
- Grundy-Warr C, "Raw attitudes, wetland cultures, lifecycles : socio-cultural dynamics relating to Opisthorchis viverrini in the Mekong Basin", Parasitol Int, 61, pp.65~70 (2012)
- Duenngai K, "Improvement of PCR for detection of Opisthorchis viverrini DNA in human stool samples", J Clin Microbiol, 46, pp.366~368 (2008)
- Keiser J, "Chemotherapy for major food-borne trematodes : a review", Expert Opin Pharmacother, 5, pp.1711~1726 (2004)
- Sasaki DT, "Discrimination of viable and non-viable cells using propidium iodide in two color immunofluorescence", Cytometry, 8, pp.413~420 (1987)
- Jones KH, "An improved method to determine cell viability by simultaneous staining with fluorescein diacetate-propidium iodide", J Histochem Cytochem, 33, pp.77~79 (1985)
- Wykoff DE, "Opisthorchis viverrini in Thailand: the life cycle and comparison with O. felineus", J Parasitol, 51, pp.207~214 (1965)
- Brockelman WY, "Field studies on the transmission of the human liver fluke, Opisthorchis viverrini, in northeast Thailand : population changes of the snail intermediate host", Int J Parasitol, 16, pp.545~552 (1986)
- Suebsoh W, "Municipal Human Excreta Management in northeast Thailand", Pak J Soc Sci, 6, pp.376~381 (2009)
- Schouw NL, "Composition of human excreta-a case study from Southern Thailand", Sci Total Environ, 286, pp.155~166 (2002)
- Fewtrell L, "Water Quality: Guidelines, Standards and Health" (2001), IWA Publishing
- Hildreth M, "Lethal effects of freezing Echinococcus multilocularis eggs at ultralow temperatures", J Parasitol, 90, pp.841~844 (2004)
- Schiller EL, "Studies on the helminth fauna of Alaska. XXVI. Some observations on the cold-resistance of eggs of Echinococcus sibiricensis Rausch and Schiller, 1954", J Parasitol, 41, pp.578~582 (1955)
- Ernst I, "Effects of temperature, salinity, desiccation and chemical treatments on egg embryonation and hatching success of Benedenia seriolae(Monogenea : Capsalidae), a parasite of farmed Seriola spp", J Fish Dis, 28, pp.157~164 (2005)
- Beyhan YE, "Effects of acetic acid on the viability of Ascaris lumbricoides eggs : Is vinegar reliable enough to clean the vegetables", Saudi Med J, 37, pp.288~ (2016)
- World Health Organization, "Guidelines for Drinking-Water Quality: Fourth Edition Incorporating the First Addendum", World Health Organization, ~ (2017)
- Collivignarelli MC, "Overview of the Main Disinfection Processes for Wastewater and Drinking Water Treatment Plants", Sustainability, 10, pp.86~ (2017)
- Arther RG, "Parasite ova in anaerobically digested sludge", J Water Pollut Control Fed, 53, pp.1334~1338 (1981)