Antioxidative activity of Chicoric acid through Nrf2/MAPK mediated HO-1 induction in RAW 264.7 cells
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The antioxidativepotential of chicoric acid was investigated through heme oxygenase (HO)-1, one of phase II enzymes and exhibits antioxidative activity, induction in RAW 264.7 cells. There were also measured intracellular radical scavenging activity and glutathione (GSH) concentration in chicoric acid treated RAW 264.7 cells. Chicoric acid treatment potently induced HO-1 expression, scavenged reactive oxygen species (ROS) and increased GSH concentration. Transcription factor of phase II enzymes, nuclear factor-erythroid 2 p45-related factor 2 (Nrf2), was investigated and identified in accordance with HO-1 expression. In addition, chicoric acid treatment phosphorylated PI3K/Akt with slight activation of the extrecellularregulated kinase (ERK). Chicoric acid-induced antioxidative potential protected RAW 264.7 cells against oxidative stress-induced cell death. In conclusion, chicoric acid potently accelerated Nrf2-mediated antioxidative potential through the modulation of PI3K/Akt pathway, which would contribute to their promising strategy for a novelanti-oxidative agent.
Abstract
The antioxidativepotential of chicoric acid was investigated through heme oxygenase (HO)-1, one of phase II enzymes and exhibits antioxidative activity, induction in RAW 264.7 cells. There were also measured intracellular radical scavenging activity and glutathione (GSH) concentration in chicoric acid treated RAW 264.7 cells. Chicoric acid treatment potently induced HO-1 expression, scavenged reactive oxygen species (ROS) and increased GSH concentration. Transcription factor of phase II enzymes, nuclear factor-erythroid 2 p45-related factor 2 (Nrf2), was investigated and identified in accordance with HO-1 expression. In addition, chicoric acid treatment phosphorylated PI3K/Akt with slight activation of the extrecellularregulated kinase (ERK). Chicoric acid-induced antioxidative potential protected RAW 264.7 cells against oxidative stress-induced cell death. In conclusion, chicoric acid potently accelerated Nrf2-mediated antioxidative potential through the modulation of PI3K/Akt pathway, which would contribute to their promising strategy for a novelanti-oxidative agent.
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