대식세포에서 Nrf2/HO-1 경로 활성을 통한 그라비올라 잎 추출물의 항산화 효과
Anti-oxidative Effect of Graviola Leaf Extracts via Activation of Nrf2/HO-1 Pathway in Macrophages
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The aim of this study was to determine the effects of Graviola leaf extracts (GLE) on the antioxidant activity in LPSstimulated RAW264.7 macrophages. To investigate the antioxidant effects of GLE, we assessed scavenging activities on DPPH and ABTS radicals. DPPH and ABTS radical scavenging activities significantly increased in a GLE dosedependent manner. GLE induced nuclear translocation of nuclear-E2-related factor 2 (Nrf2), a key regulator of HO-1 expression, in a concentration-dependent manner. The expression of heme oxygenase-1 (HO-1) was also induced in LPS-treated RAW264.7 cells. The expression of proinflammatory proteins including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were also obviously inhibited by GLE in LPS-induced RAW264.7 cells. Furthermore, the production of prostagland E2 (PGE2), interleukin-1β (IL-1β), IL-6 and tumor necrosis factor-α (TNF-α) were also remarkably inhibited in LPS-induced RAW264.7 cells following treatment with GLE. These results suggest that GLE exerted anti-oxidative effects in RAW264.7 cells through modulation of the Nrf2/HO-1 pathway and that GLE could be a new Nrf2 activator for the treatment and prevention of oxidative stress.
Abstract
The aim of this study was to determine the effects of Graviola leaf extracts (GLE) on the antioxidant activity in LPSstimulated RAW264.7 macrophages. To investigate the antioxidant effects of GLE, we assessed scavenging activities on DPPH and ABTS radicals. DPPH and ABTS radical scavenging activities significantly increased in a GLE dosedependent manner. GLE induced nuclear translocation of nuclear-E2-related factor 2 (Nrf2), a key regulator of HO-1 expression, in a concentration-dependent manner. The expression of heme oxygenase-1 (HO-1) was also induced in LPS-treated RAW264.7 cells. The expression of proinflammatory proteins including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were also obviously inhibited by GLE in LPS-induced RAW264.7 cells. Furthermore, the production of prostagland E2 (PGE2), interleukin-1β (IL-1β), IL-6 and tumor necrosis factor-α (TNF-α) were also remarkably inhibited in LPS-induced RAW264.7 cells following treatment with GLE. These results suggest that GLE exerted anti-oxidative effects in RAW264.7 cells through modulation of the Nrf2/HO-1 pathway and that GLE could be a new Nrf2 activator for the treatment and prevention of oxidative stress.
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