Anti-oxidizing effect of the dichloromethane and hexane fractions from Orostachys japonicus in LPS-stimulated RAW 264.7 cells via upregulation of Nrf2 expression and activation of MAPK signaling pathway
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초록
Orostachys japonicus shows various biological activities. However, the molecular mechanisms remain unknown inLPS-stimulated macrophages. Here, we investigated theanti-oxidizing effect of the dichloromethane (DCM) and hexanefractions from O. japonicus (OJD and OJH) against oxidativestress in RAW 264.7 cells stimulated by LPS. OJD and OJHsignificantly increased the expression of heme oxygenase-1(HO-1) in a dose- and time-dependent manner. Additionally, itwas found that the expression of HO-1 was stimulated by Nrf2activated via degradation of Keap1. ERK and p38 inhibitorsrepressed HO-1 induced by OJD and OJH in LPS-stimulatedcells, respectively. In conclusion, these results suggest that OJDand OJH may block oxidative damage stimulated by LPS, viaincreasing the expression of HO-1 and Nrf2, and MAPKsignaling pathway.
Abstract
Orostachys japonicus shows various biological activities. However, the molecular mechanisms remain unknown inLPS-stimulated macrophages. Here, we investigated theanti-oxidizing effect of the dichloromethane (DCM) and hexanefractions from O. japonicus (OJD and OJH) against oxidativestress in RAW 264.7 cells stimulated by LPS. OJD and OJHsignificantly increased the expression of heme oxygenase-1(HO-1) in a dose- and time-dependent manner. Additionally, itwas found that the expression of HO-1 was stimulated by Nrf2activated via degradation of Keap1. ERK and p38 inhibitorsrepressed HO-1 induced by OJD and OJH in LPS-stimulatedcells, respectively. In conclusion, these results suggest that OJDand OJH may block oxidative damage stimulated by LPS, viaincreasing the expression of HO-1 and Nrf2, and MAPKsignaling pathway.
참고문헌 (24)
- Kim, I. D., "Paeoniflorin protects RAW 264.7 macrophages from LPS-induced cytotoxicity and genotoxicity", Toxicol. In Vitro, 23, pp.1014~1019 (2009)
- Chow, J. M., "Quercetin, but not rutin and quercitrin, prevention of H2O2-induced apoptosis via anti-oxidant activity and heme oxygenase 1 gene expression in macrophages", Biochem. Pharmacol, 69, pp.1839~1851 (2005)
- Kim, C. K., "Taurine chloramine induces heme oxygenase-1 expression via Nrf2 activation in murine macrophages", Int. Immunopharmacology, 10, pp.440~446 (2010)
- Lee, D. S., "Anti-inflammatory effects of sulfuretin from Rhus verniciflua Stokes via the induction of heme oxygenase-1 expression in murine macrophages", Int. Immunopharmacology, 10, pp.850~858 (2010)
- Kim, J. Y., "Mutation Research", Fundamental and Molecular Mechanisms of Mutagenesis, 2010, pp.690, 12~23
- Park, S. Y., "Schisandra chinensis α-iso-cubebenol induces heme oxygenase-1 expression through PI3K", Akt and Nrf2 signaling and has anti-inflammatory activity in Porphyromonas gingivalis lipopolysaccharide-stimulated macrophages, 2011, pp.11, 1907~1915
- Park, J. C., "Changes in hepatic drug metabolizing enzymes and lipid peroxidation by methanol extract and major compound of Orostachys japonicus", J. Ethnopharmacol, 102, pp.313~318 (2005)
- 류덕선, "Effects of polysaccharides derived from Orostachys japonicus on induction of cell cycle arrest and apoptotic cell death in human colon cancer cells", BMB Reports, 43(11), pp.750~755 (2010), 생화학분자생물학회
- Jae-Han Jeong, "Anti-inflammatory effects of ethanol extract from Orostachys japonicus on modulation of signal pathways in LPS-stimulated RAW 264.7 cells", BMB Reports, 44(6), pp.399~404 (2011), 생화학분자생물학회
- Lee, H. S., "Antiinflammatory effects of dichloromethane fraction from Orostachys japonicus in RAW 264.7 cells: Suppression of NF-κB activation and MAPK signaling", J. Ethnopharmacology, 140, pp.271~276 (2012)
- Khlifi, D., "Composition and anti-oxidant, anti- cancer and anti-inflammatory activities of Artemisia herba- alba, Ruta chalpensis L. and Peganum harmala L", Food Chem. Toxicol, 55, pp.202~208 (2013)
- Lee, H. S., "Anti-inflammatory effect of the hexane fraction from Orostachys japonicus in RAW 264.7 cells by suppression of NF-κB and PI3K-Akt signaling", J. Funct. Foods, 5, pp.1217~1225 (2013)
- Yongfen Ma, "Anti-inflammatory Activities of Coumarins Isolated from Angelica gigas Nakai on LPS-stimulated RAW 264.7 Cells", Preventive Nutrition and Food Science, 14(3), pp.179~187 (2009), 한국식품영양과학회
- Copple, I. M., "The Nrf2-Keap1 defence pathway: Role in protection against drug-induced toxicity", Toxicology, 246, pp.24~33 (2008)
- Kim, A. N., "Up-regulation of heme oxygenase-1 expression through CaMKII-ERK1", 2-Nrf2 signaling mediates the anti-inflammatory effect of bisdemethoxycurcumin in LPS-stimulated macrophages, 2010, pp.49, 323~331
- 송주동, "Involvement of Peroxynitrite in NO Donor-Induced HO-1 Expression in Rat Articular Chondrocytes", 생명과학회지, 21(4), pp.486~493 (2011), 한국생명과학회
- Kim B. C., "Heme oxygenase-1 mediates the anti-inflammatory effect of mushroom Phellinus linteus in LPS-stimulated RAW 264.7 macrophages", J. Ethnopharmacol, 106, pp.364~371 (2006)
- Chung, H. T., "Interaction Relations between Nitric Oxide (NO) and Carbon Monoxide (CO): Heme Oxygenase-1", CO pathway is a key modulator in NO-mediated Antiapoptosis and Anti-inflammation, 2008, pp.441, 329~338
- Park, S. Y., "Upregulation of heme oxygenase-1 via PI3K", Akt and Nrf2 signaling pathways mediates the anti-inflammatory activity of Schisandrin in Porphyromonas gingivalis LPS-stimulated macrophages, 2011, pp.139, 93~101
- Lee, J. M., "An Important Role of Nrf2-ARE Pathway in the Cellular Defense Mechanism", J. Biochem. Mol. Biol, 37, pp.139~143 (2004)
- Stepkowski, T. M., "Molecular cross-talk between the NRF2", KEAP1 signaling pathway, autophagy, and apoptosis, 2011, pp.50, 1186~1195
- Fan, W., "Keap1 facilitates p62- mediated ubiquitin aggregate clearance via autophage", Autophagy, 6, pp.614~621 (2010)
- Zahid Manzoor, "Mitogen-activated Protein Kinases in Inflammation", Journal of Bacteriology and Virology, 42(3), pp.189~195 (2012), 대한미생물학회
- 조장은, "Mycobacterium tuberculosis-induced expression of granulocyte-macrophage colony stimulating factor is mediated by PI3-K", MEK1, BMB Reports, pp.2013, 46(4)[DOI]
