인간치은섬유아세포의 다양한 세포행동 관련 유전자발현에 마이크로그루브-파이브로넥틴 복합 티타늄표면이 미치는 영향에 대한 연구
A study on the effect of microgroove-fibronectin complex titanium plate on the expression of various cell behavior-related genes in human gingival fibroblasts
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목적:인간 치은섬유아세포의 세포활동에 관련된 다양한 유전자들의 발현에 마이크로그루브-파이브로넥틴 복합 표면이미치는 영향을 확인하고자 하였다. 연구 재료 및 방법: 평활한 티타늄시편(NE0), 산처리만 시행한 티타늄시편(E0), 마이크로그루브 및 산처리된 티타늄시편(E60/10), 파이브로넥틴을 고정시킨 평활한 티타늄시편(NE0FN), 산처리 및 파이브로넥틴을 고정시킨 티타늄시편(E0FN), 그리고 마이크로그루브 및 산처리 후 파이브로넥틴을 고정시킨 티타늄시편(E60/10FN) 실험군 제작 후 인간치은섬유아세포의 세포행동 관련 44개 유전자에 대한 실시간 중합효소연쇄반응 실험을 진행하였다. 결과: 인간치은섬유아세포 부착과 증식 등에 관여하는 4종류 신호전달 경로가 활성화되었다. Focal adhesion 경로에 속하는 Integrin α5, Integrin β1, Integrin β3, Talin-2 유전자들, PI3K-AKT 신호 전달 경로에 속하는 AKT1, AKT2, NF-κB 유전자들, MAPK 신호전달 경로에 속하는 MEK2, ERK1, ERK2 유전자들, 세포주기 신호 전달 경로에 속하는 cyclinD1, CDK4, CDK6 유전자들이 마이크로그루브-파이브로넥틴 복합 티타늄표면(E60/10FN)에서 상향조절되었다. 결론: 마이크로그루브-파이브로넥틴 복합 티타늄표면이 세포행동에 관여하는 다양한 유전자들을 상향조절할 수 있다.
Abstract
Purpose: To determine the effects of the microgroove-fibronectin complex surface on the expression of various genes related to cellularactivity in human gingival fibroblasts. Materials and Methods: Smooth titanium specimens (NE0), acid-treated titanium specimens(E0), microgroove and acid-treated titanium specimens (E60/10), fibronectin-fixed smooth titanium specimens (NE0FN), acidtreatedand fibronectin-immobilized titanium specimens (E0FN), and microgroove and acid-treated titanium specimens immobilizedwith fibronectin (E60/10FN) were prepared. Real-time polymerase chain reaction experiments were conducted on 44 genes relatedto cell behavior of human gingival fibroblasts. Results: Adhesion and proliferation of human gingival fibroblast on microgroovefibronectincomplex titanium were activated through four types of signaling pathway. Integrin α5, Integrin β1, Integrin β3, Talin-2,which belong to the focal adhesion pathway, AKT1, AKT2, NF-κB, which belong to the PI3K-AKT signaling pathway, MEK2, ERK1,ERK2, which belong to the MAPK signaling pathway, and Cyclin D1, CDK4, CDK6 genes belonging to the cell cycle signaling pathwaywere upregulated on the microgroove-fibronectin complex titanium surface (E60/10FN). Conclusion: The microgroove-fibronectincomplex titanium surface can up-regulate various genes involved in cell behavior.
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