내건성 식물의 기내증식에 미치는 배양배지와 생장조절제의 영향
Influence of Culture Medium and Plant Growth Regulators on in vitro Propagation of Drought Tolerant Plants
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내건성이 우수하다고 판명되어진 비수리(Lespedeza cuneata), 새(Arundinella hirta), 소리쟁이(Rumex crispus), 장구채(Silene firma), 매듭풀(Kummerowia striata) 5수종의 기내대량증식 조건을구명하였다. 5종의 선발종 중 비수리는 WPM배지에서 길이생장이 가장 좋았으며, 새는 MS배지, 소리쟁이는 LP배지에서 장구채는 LP배지, 매듭풀은 SH배지에서 좋은 길이생장을 보였다. 뿌리발생도 수종에따라 배양배지에 따라 다르게 나타났다. 비수리의 경우 기내발근은 모든 배지에서 뿌리가 발생하지 않았으며, 새는 MS배지, 소리쟁이는 WPM배지, 장구채는 MS배지, 매듭풀은 1/2MS배지에서 가장 좋은뿌리생장을 보였다. 잎 갈변은 비수리는 B5배지, 새는 WPM배지, 소리쟁이는 B5배지, 매듭풀은 LP배지에서 가장 심한 갈변을 보였다. 생존율은 비수리, 소리쟁이, 장구채는 모든배지에서 100% 생존율을보였으며, 새는 WPM배지에서 25% 생존율을, 매듭풀은 LP배지에서 25%의 낮은 생존율을 나타냈다. 선발종의 다경줄기를 유도하기 위해 cytokinin을 처리한 결과 비수리의 경우 TDZ 0.5mg/L 처리구, 새는BA 0.5mg/L, 소리쟁이는 TDZ 0.1mg/L, 장구채는 BA 0.5mg/L, 매듭풀은 BA 0.5mg/L 처리구에서가장 많은 다경줄기를 형성하였다. cytokinin 처리는 줄기생장, 기내발근 및 다경줄기 유도에 영향을미쳤다. 기내에서 배양된 식물체는 4종의 인공상토에서 성공적으로 순화되었다. 본 연구 결과들은 내건성을 가진 5종의 유용식물의 품종개발 및 대량증식에 크게 기여할 수 있을 것으로 판단된다.
Abstract
The conditions of mass propagation of five plant species such as Lespedeza cuneata, Arundinellahirta, Rumex crispus, Silene firma, and Kummerowia striata. They were proved to be droughttolerant plants and investigated. Among the five species, L. cuneata showed the best lengthgrowth in WPM medium, A. hirta showed good stem growth in MS medium, R. crispus in LPmedium, S. firma in LP medium and K. striata in SH medium, respectively. The rootdevelopments were also different depending on the culture media and the plant species. In thecase of L. cuneata, no root was induced in all media, A. hirta was the MS medium, R. crispuswas the WPM medium, S. firma was the MS medium, and K. striata was the best in the 1 /2MS medium. Leaf browning of L. cuneata, showed the highest browning in B5 medium, A. hirta in WPM medium, R. crispus in B5 medium and S. firma in LP medium. Survival rates ofL. cuneata, R. crispus and S. firma were 100% in all mediums whereas A. hirta was 25% inWPM medium, and K. striata was 25% in LP medium. In the case of treatments withcytokinins to induce multiple shoots of the selected species, 0.5mg/l TDZ treatment for L. cuneata, 0.5 mg/l BA for A. hirta, 0.1 mg/l TDZ for R. crispus, 0.5mg/l BA for S. firma, K. striata formed the largest number of multiple shoots at 0.5mg/l BA treatment. The cytokinintreatment affected stem growth, in vitro rooting and multiple shoot induction. The in vitrocultivated plants were successfully acclimated in four artificial soils. The results of this studycould be contributed greatly to the development and mass propagation of 5 kinds of usefulplants and drought tolerant plants.
참고문헌 (39)
- Aboubakar IM, "Saad och Ahmed M and Elshahed" (2012), Plant Tissue Culture Media
- Ali G, "Callus induction and in vitro complete plant regeneration of different cultivars of tobacco (Nicotiana tabacum L.) on media of different hormonal concentrations", Biotechnology, 6, pp.561~566 (2007)
- Bennett IJ, "Alternating cytokinins in multiplication media stimulates in vitro shoot growth and rooting of Eucalyptus globulus Labill", Ann. Bot, 74, pp.53~58 (1994)
- Bollmark M, "Variation in endogenous cytokinin content during adventitious root formation in pea cuttings", J. of Plant Physiology, 132, pp.262~265 (1988)
- Chaitanya KV, "Water stress effects on photosynthesis in different mulberry cultivars", Plant Growth Regul, 40, pp.75~80 (2003)
- Clapa D, "Tissue culture and ex-vitro acclimation of Rhododendron sp", Buletin USAMV-CN, 64 (2008)
- Cohen D., "The culture medium", Acta Hort, 393, pp.15~24 (1995)
- De Fossard RA., "Tissue culture of Eucalyptus ficifolia", Proceedings of symposium on plant tissue culture, pp.425~438 (1978), Peking Science Press
- Diallo MS, "Plants regeneration from African cowpea variety(Vigna unguiculata L. Walp.)", Af. J. Biotech, 7, pp.2828~2833 (2008)
- Fernandez GCJ., "Effective Selection Criteria for Assessing Stress Tolerance", Proceedings of the International Symposium on Adaptation of Vegetables and Other Food Crops in Temperature and Water Stress, pp.257~270 (1992), AVRDC Publication
- Fernandez JA, "Induction of drought tolerance by paclobutrazol and irrigation deficit in Phillyrea angustifolia during the nursery period", Sci. Hortic, 107, pp.277~283 (2006)
- Gamborg OL, "Nutrient requirements of suspension cultures of soybean root cells", Exp. Cell Res, 50, pp.151~158 (1968)
- Georgieva K, "Photosynthetic characteristics of transformed tobacco plants grown in vitro after tgeir transplantation in natural conditions", Plant physiol, 22, pp.3~13 (1996)
- Mahdiyeh Gholami, "Effect of Irrigation Regimes on Water Status and Photosynthetic Parameters of Peach-Almond Hybrid (GF677) Seedlings and Cuttings", Horticulture, Environment, and Biotechnology, 50(2), pp.94~99 (2009), 한국원예학회
- Grąbkowska R, "Influence of thidiazuron(TDZ) pretreatment of shoot tips on shoot multiplication and ex vitro acclimatization of Harpagophytum procumbens", Acta. Physiologiae Plantarum, 36, pp.1661~1672 (2014)
- 한은희, "장백도라지의 대량 증식을 위한 조직배양 및 순화 조건 확립", Journal of Plant Biotechnology, 41(3), pp.134~139 (2014), 한국식물생명공학회
- Hura T, "Physiological and biochemical tools useful in drought-tolerance detection in genotypes of winter triticale: accumulation of ferulic acid correlates with drought tolerance", Ann. Bot, 100, pp.767~775 (2007)
- 임현정, "생리적 인자 분석을 통한 내건성 식물 선발", 농업생명과학연구, 50(1), pp.33~43 (2016), 농업생명과학연구원[DOI]
- 임현정, "자생식물로부터 내건성 식물의 최적인자 선발과 생육특성", 농업생명과학연구, 50(2), pp.73~82 (2016), 농업생명과학연구원[DOI]
- Jahan AA, "Preconditioning of axillary buds in thidiazuron-supplemented liquid media improves in vitro shoot multiplication in Nyctanthes arbor-tristis L", Appl Biochem Biotech, 163, pp.851~859 (2011)
- Jung S., "Variation in antioxidant metabolism of young and mature leaves of Arabidopsis thaliana subjected to drought", Plant Sci, 166, pp.459~466 (2004)
- Lloyd G, "Commercially feasible micro-propagation of mountain laurel, Kalmia latifolia, by use of shoot tip culture", Int. Plant Prop. Soc. Proc, 30, pp.421~427 (1981)
- Mirbahar AA, "Effect of water stress on yield and yield components of wheat(Triticum aestivum L.) varieties", Pak. J. Bot, 41, pp.1303~1310 (2009)
- Mukherjee P, "In vitro propagation of a grape rootstock, deGrasset(Vitis champinii Planch.): Effects of medium compositions and plant growth regulators", Scientia Horticulturase, 126, pp.13~19 (2010)
- Murashige T, "A revised medium for rapid growing and bioassay with tobacco tissue culture", Physiol Plant, 15, pp.473~497 (1962)
- Nemeth G., "Benzyladenine-stimulated rooting in fruit tree rootstocks cultured in vitro", Z Pflanzenphysiol, 95, pp.389~396 (1979)
- Niu G, "Growth and physiological responses to drought stress in four oleander clones", J. Am. Soc. Hortic. Sci, 133, pp.188~196 (2008)
- Passioura JB., "Roots and drought resistance", Agric. Water Manage, 7, pp.265~280 (1983)
- Quoirin M, "Improved medium for in vitro culture of Prunus sp", Acta Hort, 78, pp.437~442 (1977)
- Romero P, "Effect of regulated deficit irrigation during the pre-harvest period on gas exchange, leaf development and crop yield of mature almond trees", Tree Physiol, 24, pp.303~312 (2004)
- RoReddy AR, "Drought induced responses of photosynthesis and antioxidant metabolism in higher plants", J. Plant Physiol, 161, pp.1189~1202 (2004)
- Saha S, "Synergistic effect of kinenin and benzyl adenine plays a vital role in high frequency regeneration from cotyledon explants of bottle gourd(Lagenaria siceraria) in relation to ethylene production", Breed. Sci, 57, pp.197~202 (2007)
- Schenk RU, "Medium and techniques for induction and growth of monocotyledonous and plant cell cultures", Can. J. Bot, 50, pp.199~204 (1972)
- Shirin F, "In vitro plantlet regeneration from nodal explants of field-grown culms in Bambusa glaucescens wild", Plant Biotechnol. Rep, 1, pp.141~147 (2007)
- Siddique I, "Rapid micropropagation of Ocimum basilicum using shoot tip explants pre-cultured in thidiazuron supplemented liquid medium", Biol. Plant, 51, pp.787~790 (2007)
- Singh SK, "A short pre-culture soak in thidiazuron or for chlorfenuron improves axillary shoot proliferation in rose micropropagation", Sci. Hortic, 91, pp.169~177 (2001)
- Tazawa K, "Multiple bud formation and plant regeneration in anther culture of Shiode(Smilax oldhami Miq.)", Breed. Sci, 53, pp.183~185 (2003)
- Tuskan GA, "Influence of plant growth regulators, basal media and carbohydrate lavels on the in vitro development of Pinus ponderosa(Dougl. ex Law) cotyledon explants", Plant Cell Tissue Organ Cult, 20, pp.47~52 (1990)
- Vankova R, "Effects of synthetic cytokinins and respiration patterns of Beta vulgaris cells in suspension", J. of Plant Growth Regulation, 10, pp.197~199 (1991)