희귀식물 제주황기의 미세번식
Micropropagation of a rare plant species, Astragalus membranaceus Bunge var. alpinus N.
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초록
In order to develop an efficient in vitro micropropagationtechnique for a rare plant species, Astragalusmembranaceus Bunge var. alpinus N., shoot proliferationand in vitro or in vivo rootings were conducted andhyperhydrated leaf generated from cultures was histologicallyobserved. During shoot induction, no distinct effect onmultiple shoot induction was found between BA and kinetintreatment. BA enhanced the number of internodes, whereaskinetin stimulated shoot elongation. Hyperhydrated leafcomposed of bigger cells and retarded palisade parenchymaand showed irregular cell arrangement compared to normalleaf. Especially starch content in hyperhydrated leaf wassignificantly reduced. The best rooting rate was achieved byB5 medium among three different medium (B5, MS andWPM) and 0.1mg/L IBA treatment induced the highestrooting ratio (80%). No statistical difference was induced byexplant types (apical bud or axillary bud) in terms of rootingratio. In vivo cutting induced rooting rate up to 65% by 0.5%IBA/Talc powder treatment. Although in vivo rooting ratewas less efficient compared to in vitro rooting, better survivalrate was observed after soil acclimatization. Present studysuggested that above micropropagation techniques can beused for rapid multiplication as well as in vitro or in vivoconservation of the species.
Abstract
In order to develop an efficient in vitro micropropagationtechnique for a rare plant species, Astragalusmembranaceus Bunge var. alpinus N., shoot proliferationand in vitro or in vivo rootings were conducted andhyperhydrated leaf generated from cultures was histologicallyobserved. During shoot induction, no distinct effect onmultiple shoot induction was found between BA and kinetintreatment. BA enhanced the number of internodes, whereaskinetin stimulated shoot elongation. Hyperhydrated leafcomposed of bigger cells and retarded palisade parenchymaand showed irregular cell arrangement compared to normalleaf. Especially starch content in hyperhydrated leaf wassignificantly reduced. The best rooting rate was achieved byB5 medium among three different medium (B5, MS andWPM) and 0.1mg/L IBA treatment induced the highestrooting ratio (80%). No statistical difference was induced byexplant types (apical bud or axillary bud) in terms of rootingratio. In vivo cutting induced rooting rate up to 65% by 0.5%IBA/Talc powder treatment. Although in vivo rooting ratewas less efficient compared to in vitro rooting, better survivalrate was observed after soil acclimatization. Present studysuggested that above micropropagation techniques can beused for rapid multiplication as well as in vitro or in vivoconservation of the species.
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