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楡白皮 추출물이 인간의 피부 세포 재생 및 악성 흑색종 세포에 미치는 영향

Effects of Cortex Ulmi pumilae on Human HaCaT keratinocyte and its antioxidant and anti-cancer effect

최정화|김종한|박수연|한진근
대한한방안이비인후피부과학회 (The Korean Medicine Opthalmology&Otolaryngology&Dermatology Society)|2007.12|한방안이비인후피부과학회지 (The Journal of Korean Medicine Ophthalmology&Otorhinolaryngology&Dermatology)|제20권 제3호|pp. 81-97

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참고문헌

주제분류한의학

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Abstract

Objective : Cortex of Ulmi pumilae(CUP) has been used to treat several diseases including boil, swelling, and scabies etc. Recently, CUP was known to have wrinkle care and whitening actions. But, It's exact mechanisms are unclear. Methods : The present study was designed to investigate effects of CUP on Human HaCaT keratinocyte and malignant melanoma cells such as SK-MEL-2 and B16F10 in terms of cell viabilities, proliferations, DPPH free radical scavenging activities, oxygen free radical productions and inhibitory action on elastase activities. Results : CUP accelerated proliferation of HaCaT keratinocytes in the lower concentration. CUP also prevented cell death of HaCaT induced by Hydrogen peroxide, which products oxygen free radicals. On the contrary, CUP did not affect proliferations of SK-MEL-2 or B16F10. Futhermore, CUP showed inhibitory action against SK-MEL-2 proliferation at the concentration of 500 ㎍/㎖. In addition, CUP was shown to have DPPH free radical scavenging activities and also have inhibitory effects on elastase activities too. On the fluorescent examinations, the present author knows that CUP elevated production levels of oxygen free radicals in malignant melanoma cell, SK-MEL-2. Conclusions : These results suggest that CUP has possibilities of usage for functional cosmetics which have wrinkle care and whitening activities and related mechanisms are involved in inhibition of elastase action and acceleration of oxidative stress in melanoma cell.

참고문헌 (37)

  1. "神農本草經 中藥彩色图譜", pp.150~151 (1996), 北京:中國中醫藥出版社
  2. "陳師文 編. 太平惠民和劑局方", . 旋風出版社. 192.
  3. "楡白皮와 刺楡皮의 形態 鑑別에 관한 硏究", 19(2), pp.169~179 (2004)
  4. "김진식. 楡白皮의 성분에 관한 연구. 생약학회지. 1990", pp.201~4
  5. "Dictionary of Chinese Medicines", pp.4319~4321 (1998), Seoul:Jeong Dam Press
  6. "J.H. Park and O.P. Jee. Catechin glycoside from ulmus davidiana", pp.193~
  7. "N.J. Kim and J.S. Kim. A study on efficacy of ulmi cortex", pp.217~22
  8. "Antibacterial Activity of ulmus pumila L. Extract. 산업미생물학회지. 1992", pp.1~5
  9. "楡白皮가 항염작용에 미치는 영향", 7(1), pp.837~852 (1998)
  10. "楡白皮 (Ulmi cortex) 의 항균활성", 28(5), pp.1022~1028 (1999)
  11. "楡白皮 (Ulmi cortex) 추출물의 항산화 활성", 7(4), pp.373~379 (2000)
  12. "楡白皮, 계지, 우슬, 봉독 및 우황, 웅담, 사향 복잡제제 약침이 mouse 의 LPS 유발 관절염의 혈액학적 변화에 미치는 영향", 18(1), pp.157~169 (2001)
  13. "楡白皮 물 추출물이 흰쥐의 위궤양에 미치는 영향", 10(3), pp.177~180 (2002)
  14. "楡白皮 추출물을 함유한 하이드로겔 패치의 주름 억제 효과", 34(3), pp.193~199 (2004)
  15. "Experimental study on the anti-inflammatory and wound healing effect of ulmus parvifolia, Research Institute of Korean Medicine Thesid Collection", pp.837~852 (1998), Taejeon University
  16. "Clark GM. Predictive value of trypan blue exclusion viability measurements for colony formation in a human tumor cloning assay. Cancer Drug Deliv. 1984", pp.95~100
  17. "King GM. Application of a Tetrazolium Salt with a Water-Soluble Formazan as an Indicator of Viability in Respiring Bacteria. Appl Environ Microbiol. 1993", pp.2891~6
  18. "Hydrogen peroxide induces necrosis, apoptosis, oncosis and apoptotic oncosis of mouse terminal proximal straight tubule cells", pp.234~238 (1999), Nephron
  19. "Hydrogen peroxide inhibits human keratinocyte migration", pp.525~529 (1996), Dermatol Surg
  20. "Free radical scavenging activity in the nonenzymatic fraction of human saliva: a simple DPPH assay showing the effect of physical exercise", pp.537~546 (1999), Antioxid Redox Signal
  21. "Inhibition of human leukocyte and porcin pancreatic elastase by homologues of bovine pancreatic tyrosin inhibitors", pp.9090~9096 (1996)
  22. "Active oxygen chemistry within the liposomal bilayer. Part IV: Locating 2',7'-dichlorofluorescein (DCF), 2',7'-dichloro -dihydrofluorescein (DCFH) and 2',7'-dichlorodihydro －fluorescein diacetate (DCFH-DA) in the lipid bilayer", 131(1), pp.123~133 (2004)
  23. "Adrenocorticotropin hormone stimulates interleukin-18 expression in human HaCaT keratinocytes", 127(5), pp.1210~1216 (2007)
  24. "Petrotetrayndiol A induces cell cycle arrest and apoptosis in SK-MEL-2 human melanoma cells through cytochrome c-mediated activation of caspases", 232(2), pp.214~225 (2006)
  25. "Polyhemoglobin-tyrosinase, an oxygen carrier with murine B16F10 melanoma suppression properties : a preliminary report", 32(2), pp.293~302 (2004)
  26. "Phothoaging is associated with protein oxidation in human skin in vivo", pp.618~625 (2002)
  27. "Reactive oxygen species as mediators of UVB-induced mitogen activated protein kinase activation in kerativnocytes", pp.114~127 (2001)
  28. "Free radicals and antioxidants in normal physiological functions and human disease", 39(1), pp.44~84 (2007)
  29. "Development of an Agar Diffusion Method to Measure Elastase Inhibition Activity Using Elastin-Congo Red", 16(8), pp.1320~1324 (2006)
  30. "Neutrophil Elastase Causes MUC5AC Mucin Synthesis Via EGF Receptor, ERK and NF-kB Pathways in A549 Cells", 20(4), pp.275~283 (2005)
  31. "감태(Ecklonia cava)에서 분리한 Phlorotannin 성분의 Elastase 저해활성 및 Hyaluronidase 저해활성", 27(2), pp.92~96 (2006)
  32. "The role of elastases secreted by fibroblasts in wrinkle formation : implication through selective inhibition of elastase activity", 74(2), pp.283~290 (2001)
  33. "Cheronis JC. Inhibition of human leucocyte elastase by ursolic acid. Evidence for a binding site for pentacyclic triterpenes. Biochem J. 1991", pp.521~6
  34. "Mitochondrial oxidative stress: implications for cell death", 47, pp.143~183 (2007)
  35. "An anesthetic possessing neuroprotective action against oxidative stress, promotes the process of cell death induced by H2O2 in rat thymocytes", pp.18~23 (2006)
  36. "Ascorbate potentiates the cytotoxicity of menadione leading to an oxidative stress that kills cancer cells by a non-apoptotic caspase-3 independent form of cell death", 9(2), pp.223~233 (2004)
  37. "Depletion of endogenous zinc stores induces oxidative stress and cell death in human melanoma cells", 47(2), pp.91~96 (2004)

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