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龍膽草 추출물이 LPS로 활성화된 Raw 264.7 cell에서의 pro-inflammatory mediator에 미치는 영향

Inhibitory effect of Gentianae Radix MeOH extract on pro-inflammatory mediator production in lipopolysaccharide activated Raw 264.7 cells

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초록

In traditional oriental medicine, Gentianae Radix has been used clinically for clearing away 'heat', removing dampness and purging fire in the liver and gall bladder. However, there has been a lack of studies regarding the effects of Gentianae Radix on the immunological activities. The present study was conducted to evaluate the effect of Gentianae Radix on the regulatory effects of cytokines and nitric oxide(NO) for the immunological activities in Raw 264.7 cells. After the treatment of Gentianae Radix MeOH extract, cell viability was measured by MTT assay, and NO production was monitored by measuring the nitrite content in culture medium. The expression of COX-2 and iNOS was determined by immunoblot analysis, and the content of levels of cytokines in media was analyzed by ELISA kit. Results provided evidence that Gentianae Radix inhibited the production of nitrite and nitrate (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β) and IL-6, and the activation of phospholylation of inhibitor κBα (p-IκBα) in Raw 264.7 cells activated with lipopolysaccharide (LPS). These findings suggest that Gentianae Radix can make anti-inflammatory effect, which may play a role in adjunctive therapy.

Abstract

In traditional oriental medicine, Gentianae Radix has been used clinically for clearing away 'heat', removing dampness and purging fire in the liver and gall bladder. However, there has been a lack of studies regarding the effects of Gentianae Radix on the immunological activities. The present study was conducted to evaluate the effect of Gentianae Radix on the regulatory effects of cytokines and nitric oxide(NO) for the immunological activities in Raw 264.7 cells. After the treatment of Gentianae Radix MeOH extract, cell viability was measured by MTT assay, and NO production was monitored by measuring the nitrite content in culture medium. The expression of COX-2 and iNOS was determined by immunoblot analysis, and the content of levels of cytokines in media was analyzed by ELISA kit. Results provided evidence that Gentianae Radix inhibited the production of nitrite and nitrate (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β) and IL-6, and the activation of phospholylation of inhibitor κBα (p-IκBα) in Raw 264.7 cells activated with lipopolysaccharide (LPS). These findings suggest that Gentianae Radix can make anti-inflammatory effect, which may play a role in adjunctive therapy.

참고문헌 (39)

  1. Mc daniel ML, "Cytokines and nitric oxides in islet inflammation and diabetes", Proc. Soc. Exp. Biol. Med, 211, pp.24~32 (1996)
  2. Higuchi M, "Cytolytic mechanism of activated macrophages. Tumor necrosis factor and L-argininedependent mechanism acts as synergistically as the major cytolytic mechanism of activated macrophages", J Immunol, 144, pp.1425~31 (1990)
  3. Corbett JA, "Intraislet release of interleukin-1 inhibits beta cell function by inducing beta cell expression of inducible nitric oxide syntheses", J Exp Med, 181, pp.559~68 (1995)
  4. Cetkovic-Cvrlje M, "TNF and IFNγ potentiate the deleterious effects of IL-1β on mouse pancreatic islets mainly via generation of nitric oxide", Cytokine, 6, pp.399~406 (1994)
  5. Kawamata H, "Enhanced expression of inducible nitric oxide synthase by Juzentaiho- to in LPS-activated RAW 264.7 cells, a murine macrophage cell line", Am J Chin Med, 28, pp.217~26 (2000)
  6. Lee BG, "Suppression of inducible nitric oxide synthase expression in RAW 264.7 macrophages by two- carboline alkaloids extracted from Melia azedarach", Eur J Pharmacol, 406, pp.301~9 (2000)
  7. Seo WG, "Inhibitory effects of methanol extract of Cyperus rotundus rhizomes on nitric oxide and superoxide production by murine macrophage cell line, RAW 264.7 cells", J Ethnopharmacol, 76, pp.59~64 (2001)
  8. 이영선, "향부자 열수추출물의 Nitric oxide 생성 및 iNOS 유전자 발현에 미치는 영향", 동의생리병리학회지, 17(3), pp.771~776 (2003), 대한동의병리학회
  9. Seo WG, "Inhibitory effect of ethyl acetate fraction from Cudrania tricuspidat on the expression of nitric oxide synthase gene in RAW 264.7 macrophages stimulated with interferon and lipopolysaccharide", Gen Pharmacol, 35, pp.21~8 (2000)
  10. 장선일, "활성화된 설치류 RAW 264.7 대식세포에서 당귀 에탄올 추출물의 항염증 효과", 대한한의학방제학회지, 10(2), pp.189~97 (2002)
  11. Chun SC, "Anti-Inflammatory Activity of the ethanol Extract of Moutan Cortex in LPS-Activated Raw 264.7 Cells", eCAM, 4(3), pp.327~33 (2007)
  12. Lim JH, "Inhibitory effect of Yongdamsagantang water extract on IL-6 and nitric oxide production in lipopolysaccharide-activated RAW 264.7 cells", OPEM, 7(3), pp.321~9 (2007)
  13. 김창민, "중약대사전", pp.2836~ (1998), 서울:정담
  14. 전국한의대본초학교수, "본초학", pp.184~ (1992), 서울:영림사
  15. 木村正康, "漢方藥理學", pp.364~5 (1997), 南山堂:東京
  16. 한방약리학 교재편찬위원회, "한방약리학", pp.594~5 (2006), 서울:신일상사
  17. 한상원, "龍膽草(龍膽草) 및 시호수침(柴胡水鍼)이 CCl4 로 유발된 흰쥐의 손상 간에 미치는 영향", 대한침구학회지, 10(1), pp.297~313 (1993)
  18. Tang ZM, "Screening 20 Chinese herbs often used for clearing heat and dissipating toxin with nude mice model of hepatitis C viral infection", Zhongguo Zhong Xi Yi Jie He Za Zhi, 23(6), pp.447~8 (2003)
  19. Kumazawa N, "Protective effects of various methanol extracts of crude drugs on experimental hepatic injury induced by alpha-naphthylisothiocyanate in rats", Yakugaku Zasshi, 111(3), pp.199~204 (1991)
  20. 문성호, "농도별 초용담(草龍膽) 수침이 담석 유발 생쥐에 미치는 영향", 대한침구학회지, 8(1), pp.141~56 (1991)
  21. 김나영, "용담의 RAW264.7 세포주에서의 Nitric Oxide 생성 저해물질", 생약학회지, 30(2), pp.173~6 (1999)
  22. Palmer RM, "Vascular endothelial cells synthesize nitric oxide from L-arginine", Nature, 333, pp.664~6 (1988)
  23. 임동건, "산화 스트레스; 활성산소종과 산화질소", 대한중환자의학회지, 19(2), pp.81~5 (2004)
  24. Kubes P, "Inducible nitric oxide synthase; a little bit og good in all of us", Gut, 47, pp.6~9 (2000)
  25. Chiou WF, "Camptothecin suppresses nitric oxide biosynthesis in RAW 264.7 macrophages", Life Sci, 69, pp.625~35 (2001)
  26. Seo WG, "The aqueous extract of Rhodiola sachalinensis root enhances the expression of inducible nitric oxide synthase gene in RAW 264.7 macrophages", J Ehnopharmacol, 76, pp.119~23 (2001)
  27. Surh YJ, "Anti-tumor promoting potential of selected spice ingredients with antioxidative and anti-inflammatory activities: a short review", Food Chem Toxicol, 40(8), pp.1091~7 (2002)
  28. Surh YJ, "Molecular mechanisms underlying chemopreventive activities of anti-inflammatory phytochemicals: down-regulation of COX-2 and iNOS through suppression of NF-kappa B activation", Mutat Res, 480-481, pp.243~68 (2001)
  29. Linton MF, "Cyclooxygenase-2 and inflammation in atherosclerosis", Curr Opin Pharmacol, 4(2), pp.116~23 (2004)
  30. Lee AK, "Inhibition of lipopolysaccharide-inducible nitric oxide synthase, TNF-α and COX-2 expression by sauchinone effects on I-κB α phosphorylation, C", EBP and AP-1 activation, 2003, pp.139, 11~20
  31. Chun K, "Nitric oxide induces expression of cyclooxygenase-2 in mouse skin through activation of NF-κB", Carcinogenesis, 25(3), pp.445~54 (2004)
  32. 박혜준, "五味消毒飮이 마우스 腹腔 大食細胞에서 NO의 分泌過程에 미치는 영향", 동의생리병리학회지, 16(5), pp.15~927 (2002), 대한동의병리학회
  33. Victor VM, "Role of free radicals in sepsis: antioxidant therapy", Curr Pharm Des, 11(24), pp.3141~58 (2005)
  34. Chen F, "New insights into the role of nuclear factorkappaB in cell growth regulation", Am J Pathol, 159(2), pp.387~97 (2001)
  35. Delgado AV, "Production of tumor necrosis factor-alpha, interleukin 1-beta, interleukin 2, and interleukin 6 by rat leukocyte subpopulations after exposure to substance P", Neuropeptides, 37(6), pp.355~61 (2003)
  36. Casey LC, "Plasma cytokine and endotoxin levels correlate with survival in patients with the sepsis syndrome", Ann Intern Med, 119, pp.771~8 (1993)
  37. Wogensen L, "Pancreatic beta-cell function and interleukin- 1b in plasma during the acute phase response in patients with major burn injuries", Eur J Clin Invest, 23, pp.311~9 (1993)
  38. Shito M, "Interleukin 1 receptor blockade reduces tumor necrosis factor production, tissue injury, and mortality after hepatic ischemiareperfusion in the rat", Transplantation, 63, pp.143~8 (1997)
  39. Takabayashi T, "Interleukin-1 upregulates anaphylatoxin receptors on mononuclear cells", Surgery, 135(5), pp.544~54 (2004)

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