Mouse cell에서 仙方活命飮의 항산화작용과 항알러지 및 항염증 효과
The Effects of Sunbanghwalmyung-eum Extract on Anti-oxidant, Anti-allergic and Anti-inflammatory ability in mouse cell
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Background and Objectives:The aim of this study was to investigate the anti-oxidant, anti-allergic and anti-inflammatory ability of the Taglisodog-eum(SHE) extract on the RAW 264.7 and EL4 cells Materials and Methods:Three types of experiments were implemented for this study: first, the experiment to study the anti-oxidant effect of SHE using Riboflavin; second, in vitro experiment to investigate the inhibition of Th 2 cell differentiation by SHE using EL4 cells (IL-4 mRNA expression); third, the suppression of NF-κB activation using RAW 264.7 cells (iNOS and COX-2 mRNA expression). Results:The anti-oxidant ability of SHE were dose-dependantly increased. From in vitro, the LPS-induced iNOS and COX-2 mRNA expression were dose-dependantly decreased in the RAW264.7 cells treated with SHE and the PMA-induced IL-4 mRNA expression were also dose-dependantly decreased in EL4 cells. NF-κB activation was suppressed, and iNOS & COX-2 production were inhibited by SHE Conclusion:The results suggest that SHE has dose-dependant anti-oxidant ability, and has anti-allergic and anti-inflammatory effects through the suppression of NF-κB activation and the inhibition of Th 2 cell differentiation.
Abstract
Background and Objectives:The aim of this study was to investigate the anti-oxidant, anti-allergic and anti-inflammatory ability of the Taglisodog-eum(SHE) extract on the RAW 264.7 and EL4 cells Materials and Methods:Three types of experiments were implemented for this study: first, the experiment to study the anti-oxidant effect of SHE using Riboflavin; second, in vitro experiment to investigate the inhibition of Th 2 cell differentiation by SHE using EL4 cells (IL-4 mRNA expression); third, the suppression of NF-κB activation using RAW 264.7 cells (iNOS and COX-2 mRNA expression). Results:The anti-oxidant ability of SHE were dose-dependantly increased. From in vitro, the LPS-induced iNOS and COX-2 mRNA expression were dose-dependantly decreased in the RAW264.7 cells treated with SHE and the PMA-induced IL-4 mRNA expression were also dose-dependantly decreased in EL4 cells. NF-κB activation was suppressed, and iNOS & COX-2 production were inhibited by SHE Conclusion:The results suggest that SHE has dose-dependant anti-oxidant ability, and has anti-allergic and anti-inflammatory effects through the suppression of NF-κB activation and the inhibition of Th 2 cell differentiation.
참고문헌 (25)
- Stanley L, "Text Book of Path. 2nd", pp.61~5 (1963), W.B. Saunders Company
- 陳自明, "婦人良方校注補遺", pp.637~ (1991), 上海:上海科學技術出版社
- 이재희, "도설한방진료요방", pp.424~5 (1976), 서울:의학연구사
- 안봉전, "선방활명음의 항암 및 항산화효과 검증", 대한본초학회지, 20(3), pp.51~58 (2005), 대한본초학회
- 이범용, "선방활명음의 항균효능 및 성분에 관한 연구", 대한한의학회지, 19(1), pp.89~99 (1998)
- 최인화, "선방활명음의 항암 및 면역반응에 관한 실험적 연구", 경희대논문집, 15, pp.341~59 (1992)
- 안현주, "선방활명음 및 구성약물의 세포독성에 관한 실험적 연구", 한방안이비인후피부과학회지, 17(1), pp.131~42 (2004)
- 조수현, "선방활명음의 중이염 치험례", 한방안이비인후피부과학회지, 16(1), pp.198~205 (2003)
- 李梴, "編注醫學入門", pp.756~8 (1994), 서울:의성당
- 허준, "동의보감Ⅲ 제3판", pp.375~6 (1999), 서울:대성문화사
- 이상인, "방제학", pp.114~5 (1994), 서울:영림사
- Gardner DR, "Superoxide sensitivity of Escherichiacoli 6-phosphogluconate dehydratse", J.Biol. Chem, 266, pp.1478~83 (1991)
- Imlay IA, "DNA damage and oxygen radical toxicity", Science, 232, pp.1302~9 (1986)
- Baeuerle P. A, "IκB-NF-κB structure: at the interface of inflammation control", Cell, 95, pp.729~31 (1998)
- Takeno S, "Nuclear factorkappa B activation in the nasal polyp epithelium: relationship to local cytokine gene expression", Laryngoscope, 12(1), pp.53~8 (2002)
- Moncada S, "Nitric oxide:physiology, pathophysiology, and pharmacology", Pharmacol. Rev, 43, pp.109~42 (1991)
- Marletta M. A, "Macrophage oxidation of L-arginine to nitrite and nitrate: nitric oxide is an intermediate", Biochemisry, 27, pp.8706~11 (1988)
- Needleman P, "Arachidonic acid metabolism", Annu Rev Biochem, 55, pp.69~102 (1986)
- Herschman HR, "Rostaglandin synthase", Biochim Biophys Acta, 1299, pp.125~40 (1996)
- Sano H, "Expression of cycloosygenase-1 and 2 in human colorectal cacer", Cancer Res, 55, pp.3785~9 (1995)
- Borich L, "Allergic rhinitis: Systemic inflammation and implications for management", J Allergy Clin Immunol, pp.60~2 (2002)
- Yang SH, "Decreased serum IgE level, decreased IFN-γ and IL-5 but increased IL-10 production, and suppresed cyclooxygenase 2 mRNA expression in patients with perennial allergic rhinitis after treatment with a new mixed formula of Chinese herbs", Int Immunopharmacol, 1(6), pp.1173~82 (2001)
- Chrousos GP, "Stress, chronic inflammation, and emotional and physical well-being: Concurrent effects and chronic sequelae", J Allergy Clin Immunol, 106, pp.S275~91 (2000)
- Friedmann P. S, "Pathogenesis and management of atopic dermatitis", Clin. Exp. Allergy, 25, pp.799~806 (1995)
- Koreck AI, "Rhinophototherapy: A new therapeutic tool for the management of allergic rhinitis", J Allergy Clin Immunol, 115, pp.541~7 (2005)