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용담화 에탄올 추출물의 항염증 효과에 관한 연구

Study on Anti-inflammatory Effect of Ethanol Extract of Gentianae sino-ornata

김은주|우원홍|리순화|임규상|인명희|최형욱
대한한방안이비인후피부과학회 (The Korean Medicine Opthalmology&Otolaryngology&Dermatology Society)|2015.11|한방안이비인후피부과학회지 (The Journal of Korean Medicine Ophthalmology&Otorhinolaryngology&Dermatology)|제28권 제4호|pp. 51-61

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참고문헌

키워드

IL-6iNOSCOX-2LPSGentuanae sino-ornata
주제분류한의학

초록

Objective : This research has been conducted to comparative analysis of anti-inflammatory effects to suggest a usefulness of ethanol extracts from Gentianae sino-ornata (GSO) as a functional material for inflammatory activities. Methods : Cell viability was measured by neutral red (NR) assay, and nitric oxide (NO) production in RAW264.7 cells was monitored by measuring the nitrite content in culture medium. The expressions of cyclooxigenase-2 (COX-2) was determined by western blot analysis, and Inducible nitric oxide syntase (iNOS) and cytokine were determined by reverse transcriptase-polymerase chain reaction (RT-PCR). Results : When the GSO extract was added the concentration of 5-20 ㎍/㎕, the viability of cells was maintained 90% or more at all levels. NO production was suppressed by the treatment of GSO in LPS-stimulated RAW264.7 cells. GSO inhibited the expression of iNOS, COX-2, IL-1β and IL-6 in LPS-stimulated RAW264.7 cells. Conclusions: From this results, we consider that GSO can be a useful therapeutic and preventive approach to various inflammatory diseases as a functional material for inflammatory activities.

Abstract

Objective : This research has been conducted to comparative analysis of anti-inflammatory effects to suggest a usefulness of ethanol extracts from Gentianae sino-ornata (GSO) as a functional material for inflammatory activities. Methods : Cell viability was measured by neutral red (NR) assay, and nitric oxide (NO) production in RAW264.7 cells was monitored by measuring the nitrite content in culture medium. The expressions of cyclooxigenase-2 (COX-2) was determined by western blot analysis, and Inducible nitric oxide syntase (iNOS) and cytokine were determined by reverse transcriptase-polymerase chain reaction (RT-PCR). Results : When the GSO extract was added the concentration of 5-20 ㎍/㎕, the viability of cells was maintained 90% or more at all levels. NO production was suppressed by the treatment of GSO in LPS-stimulated RAW264.7 cells. GSO inhibited the expression of iNOS, COX-2, IL-1β and IL-6 in LPS-stimulated RAW264.7 cells. Conclusions: From this results, we consider that GSO can be a useful therapeutic and preventive approach to various inflammatory diseases as a functional material for inflammatory activities.

참고문헌 (31)

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관련 논문AI추천

龍膽草 추출물이 LPS로 활성화된 Raw 264.7 cell에서의 pro-inflammatory mediator에 미치는 영향김미선, 김상찬, 박숙자, 이종록, 조원준, 지선영, 황순이대한한방안이비인후피부과학회 · 한방안이비인후피부과학회지2008 · 제21권 제2호秦艽가 항염 효과에 미치는 영향이재근, 정호준, 조미정, 조희창, 지선영대한한방안이비인후피부과학회 · 한방안이비인후피부과학회지2009 · 제22권 제3호連翹의 LPS로 유도된 Raw 264.7 Cell에서의 抗炎症 효과곡정강, 김진주, 정승기, 정희재대한한방내과학회 · 대한한방내과학회지2010 · 제31권 제2호金銀花散의 LPS로 유도된 Raw 264.7 세포에서의 항염증 효과김수진, 김연수, 지선영, 황보민대한한방안이비인후피부과학회 · 한방안이비인후피부과학회지2021 · 제34권 제3호LPS로 활성화된 Raw 264.7 Cells에서 고삼 뿌리 추출물의 항염증과 항산화 효과김범준, 김영희, 손동섭, 이동훈, 임윤영, 조대윤대한의진균학회 · 대한의진균학회지2010 · 제15권 제2호양단탕 추출물의 항염증 효과에 대한 연구김태연, 최수련, 황형서한의병리학회 · 동의생리병리학회지2020 · 제34권 제5호

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