忍冬藤이 인간 유래 악성 흑색종 세포의 유전자 발현에 미치는 영향
Effects of Lonicerae Caulis (LC) on Gene Expression of Human melanoma cells
1
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
Objective : This study was designed to investigate anti-cancer and whitening activities of Lonicerae Caulis (LC). So it was investigated the effects of LC on proliferation rates of melanoma cells, then effects on genetic profile by LC. Methods : The genetic profile for the effect of LC on human derived melanoma cell, SK-MEL-2, was measured using microarray technique, and the functional analysis on these genes were conducted. Total 441 genes were up-regulated and 830 genes down-regulated in cells treated with LC. Genes induced or suppressed by LC were all mainly concerned with basic signalling pathways, which are involved in cell growth, differentiation and migration. Especially, many genes, which are related in apoptosis and cell cycle arrest were up-regulated by treatment with LC, and genes related in cell cycle were down-regulated. Result : The network of total protein interactions were identified by using cytoscape program, and some key molecules, such as BCL2L1, SIN3A, SMAD2 and c-myc that can be used for elucidation of therapeutical mechanism of medicine in the future. Conclusion : These results suggest possibility of LC as anti-cancer drug and whitening cosmetics. In addition, it was also suggested that related mechanisms are involved in BCL2L1, SIN3A, SMAD2 and c-myc related signalling pathways.
Abstract
Objective : This study was designed to investigate anti-cancer and whitening activities of Lonicerae Caulis (LC). So it was investigated the effects of LC on proliferation rates of melanoma cells, then effects on genetic profile by LC. Methods : The genetic profile for the effect of LC on human derived melanoma cell, SK-MEL-2, was measured using microarray technique, and the functional analysis on these genes were conducted. Total 441 genes were up-regulated and 830 genes down-regulated in cells treated with LC. Genes induced or suppressed by LC were all mainly concerned with basic signalling pathways, which are involved in cell growth, differentiation and migration. Especially, many genes, which are related in apoptosis and cell cycle arrest were up-regulated by treatment with LC, and genes related in cell cycle were down-regulated. Result : The network of total protein interactions were identified by using cytoscape program, and some key molecules, such as BCL2L1, SIN3A, SMAD2 and c-myc that can be used for elucidation of therapeutical mechanism of medicine in the future. Conclusion : These results suggest possibility of LC as anti-cancer drug and whitening cosmetics. In addition, it was also suggested that related mechanisms are involved in BCL2L1, SIN3A, SMAD2 and c-myc related signalling pathways.
참고문헌 (51)
- 名醫別錄, "陶弘景集・尙志均點校", pp.50~51 (1986), 人民衛生出版社
- 전국한의과대학교수공편저, "本草學", pp.233~234 (1991), 永林社
- 鄭普變, "辛民敎共編. 鄕藥(生藥)大辭典", pp.938~940 (1990), 永林社
- Kang OH, "Inhibition of trypsin-induced mast cell activation by water fraction of Lonicera japonica", Arch Pharm Res, 27(11), pp.1141~1146 (2004)
- Kim JA, "Inhibitory effect of luteolin on TNF-αlpha-induced IL-8 production in human colon epithelial cells", Int Immunopharmacol, 5(1), pp.209~217 (2005)
- Lee JH, "Anti-inflammatory effect of the aqueous extract from Lonicera japonica flower is related to inhibition of NF-kappaB activation through reducing I-kappaBalpha degradation in rat liver", Int J Mol Med, 7(1), pp.79~83 (2001)
- Tae J, "Anti-inflammatory effect of Lonicera japonica in proteinaseactivated receptor 2-mediated paw edema", Clin Chim Acta, 330(1), pp.165~171 (2003)
- Song, "Anti-inflammatory Activity of the Major Constituents of Lonicera japonica", Arch Pharm. Res, 18(2), pp.133~135 (1995)
- 이송진, "忍冬藤 추출물에 대한 항염증 및 진통작용의 비교", 생약학회지, 25(4), pp.363~367 (1994)
- 류근호, "忍冬藤 추출물의 항천식 효과", 생약학회지, 30(4), pp.377~388 (1999)
- S Hu, "Influence of medicinal herbs on phagocytosis by bovine neutrophils", Zentralbl. Veterinarmed, Reihe, 39, pp.593~599 (1992)
- S. Ohta, "protective effects of Taiwan crude drugs on experimental liver injuries", Yakugaku Zasshi, 113, pp.870~880 (1993)
- Schuchter LM, "Primary and metastatic diseases in malignant melanoma of gastrointestinal tract", Curr Opin Oncol, 12, pp.181~185 (2000)
- 문구, "암 동서의 결합치료", pp.93~97 (1999), 신아출판사
- Choi HJ, "Petrotetrayndiol A induces cell cycle arrest and apoptosis in SK-MEL-2 human melanoma cells through cytochrome c-mediated activation of caspases", Cancer Lett, 232(2), pp.214~225 (2006)
- Blanchard AP, "High-density oligonucleotide arrays", Biosensors Bioelectron, 11, pp.687~690 (1996)
- N. Iizuka, "Comparison of gene expression profiles between hepatitis B virus- and hepatitis C virus-infected hepatocellular carcinoma by oligonucleotide microarray data on the basis of a supervised learning method", Cancer Res, 62, pp.3939~3944 (2002)
- Roslev P, "Application of a Tetrazolium Salt with a Water-Soluble Formazan as an Indicator of Viability in Respiring Bacteria", Appl Environ Microbiol, pp.2891~2896 (1993)
- Florell SR, "Preservation of RNA for functional genomic studies: a multidisciplinary tumor bank protocol", Mod Pathol, 14(2), pp.116~128 (2001)
- Weis S, "Quality control for microarray analysis of human brain samples: The impact of postmortem factors, RNA characteristics, and histopathology", J Neurosci Methods, 165(2), pp.198~209 (2007)
- Kwon KH, "Analysis of gene expression profiles in cholesteatoma using oligonucleotide microarray", Acta Otolaryngol, 126(7), pp.691~697 (2006)
- Zahurak M, "Pre-processing Agilent microarray data", BMC Bioinformatics, 8, pp.142~ (2007)
- Pavlícek A, "Free-Treefreeware program for construction of phylogenetic trees on the basis of distance data and bootstrap", jackknife analysis of the tree robustness. Application in the RAPD analysis of genus Frenkelia, 1999, pp.45(3), 97~99
- 대한피부과학회 교과서 편찬위원회, "개정4판 피부과학", pp.694~ (2001), 여문각
- 정정영, "아틀라스 피부관리학", pp.51~52 (2007), 도서출판 엠디월드
- 김은주, "赤松葉 함유 韓方化粧品이 人體 皮膚 老化에 미치는 影響 연구", 대한본초학회지, 21(1), pp.25~31 (2006), 대한본초학회
- 박성규, "滋陰丹이 皮膚의 老化에 미치는 影響", 대한본초학회지, 19(1), pp.67~76 (2004), 대한본초학회
- 김은철, "천연물에 포함된 미백성분의 추출 및 미백 효과", 한국공업화학협회, 16(3), pp.348~353 (2005)
- 안봉전, "추출��건에 따른 울금의 항산화 미백 효과", 한의학잡지, 14(3), pp.168~172 (2006)
- 장경희, "미백효과가 있는 천연물에 대한 고찰", 대전대학교 한의학연구소 논문집, 13(2), pp.289~302 (2004)
- 이진태, "율피의 생리활성 및 미백효과를 이용한 화장품신소재에 관한 연구", 대한본초학회지, 20(2), pp.27~33 (2005), 대한본초학회
- 中國醫學科學院藥物硏究所等編, "中藥志", pp.256~267 (1988), 人民衛生出版社
- 《現代中醫藥文庫》 編輯委員會, "中藥現代硏究與應用", pp.2931~2963 (1997), 學苑出版社
- 王本祥, "現代中藥藥理學", pp.204~214 (1997), 天津科學技術出版社
- 李宇彬, "中藥有效成分藥理與應用", pp.110~111 (1995), 黑龍江科學技術出版社
- 박경덕, "피부 악성 흑색종의 임상 및 병리조직학적 소견", 대한피부과학회지, 45(2), pp.149~158 (2007), 대한피부과학회
- 조용찬, "불명확한 원발부위의 전이성 대장 악성흑색종 1예", Clinical Endoscopy, 32(1), pp.62~66 (2006), 대한소화기내시경학회
- Hughes TR, "Expression profiling using microarrays fabricated by an ink-jet oligonucleotide synthesizer", Nat Biotechnol, 19, pp.342~347 (2001)
- 박대중, "三白草 에탄올추출물의 항산화능 및 피부 미백작용 연구", 대한본초학회지, 23(2), pp.193~202 (2008), 대한본초학회
- Pazos Y, "Basic aspects of ghrelin action", Vitam. Horm, 77, pp.89~119 (2007)
- Gaur U, "Regulation of proliferation, survival and apoptosis by members of the TNF superfamily", Biochem. Pharmacol, 66(8), pp.1403~1408 (2003)
- Wajant H, "Tumor necrosis factor signaling", Cell Death Differ, 10(1), pp.45~65 (2003)
- Locksley RM, "The TNF and TNF receptor superfamilies: integrating mammalian biology", Cell, 104(4), pp.487~501 (2001)
- Soucek, "Modelling Myc inhibition as a cancer therapy", Nature, 455, pp.679~683 (2008)
- Hartley JL, "DNA cloning using in vitro site-specific recombination", Genome Res, 10(11), pp.1788~1795 (2001)
- Zhang Y, "Dual mechanisms of regulation of transcription of luteinizing hormone receptor gene by nuclear orphan receptors and histone deacetylase complexes", J. Steroid Biochem. Mol. Biol, 85(1), pp.401~414 (2003)
- Fiorito F, "Pagnini U.2,3,7,8-tetrachlorodibenzop- dioxin regulates bovine herpesvirus type 1 induced apoptosis by modulating Bcl-2 family members", Apoptosis, 13(10), pp.1243~1252 (2008)
- Wrana JL, "TGF-beta receptors and signalling mechanisms", Mineral and electrolyte metabolism, 24(2), pp.120~130 (1998)
- Ida K, "EWS-FLI-1 and EWS-ERG chimeric mRNAs in Ewing's sarcoma and primitive neuroectodermal tumor", Int J Cancer, 63(4), pp.500~504 (1995)
- Peeters P, "Fusion of TEL, the ETS-variant gene 6 (ETV6), to the receptor-associated kinase JAK2 as a result of t(9;12) in a lymphoid and t(9;15;12) in a myeloid leukemia", Blood, 90(7), pp.2535~2540 (1997)
- Roberts SB, "Differential phosphorylation of the transcription factor Oct1 during the cell cycle" (1991)
