DNA chip을 이용한 조각자 추출물의 인간유래 악성 종양에 미치는 영향
Effects of Gleditsia spina (GS) water extract on Gene Expression ofHuman Melanoma cells, by using Microarry technique
1
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Objective : This study was designed to investigated effects of Gleditsia spina (GS) on human derived melanoma cells Methods : The genetic profile for the effect of medicine on human derived melanoma cells of SK-MEL-2, was measured by using microarray technique, and the functional analysis on these genes was conducted. The network of total protein interactions was measured by using cytoscape program. Results : Total 253 genes were up-regulated and 439 genes down-regulated in cells treated with GS. Genes induced or suppressed by GS were all mainly concerned with metabolic process, regulation of biological process and protein binding. Conclusion : Suggest the possibility of GS as anti-cancer drug and cosmetic agent, and also suggest that related mechanisms are involved in regulation of intra-cellular metabolism in melanoma cells.
Abstract
Objective : This study was designed to investigated effects of Gleditsia spina (GS) on human derived melanoma cells Methods : The genetic profile for the effect of medicine on human derived melanoma cells of SK-MEL-2, was measured by using microarray technique, and the functional analysis on these genes was conducted. The network of total protein interactions was measured by using cytoscape program. Results : Total 253 genes were up-regulated and 439 genes down-regulated in cells treated with GS. Genes induced or suppressed by GS were all mainly concerned with metabolic process, regulation of biological process and protein binding. Conclusion : Suggest the possibility of GS as anti-cancer drug and cosmetic agent, and also suggest that related mechanisms are involved in regulation of intra-cellular metabolism in melanoma cells.
참고문헌 (38)
- 전국한의과대학 본초학교수, "本草學", pp.440~ (1998), 서울:永林社
- 신민교, "임상본초학", pp.668~9 (2000), 서울:永林社
- 江蘇新醫學院, "中藥大辭典(上)", pp.1144~7 (1980), 서울:成輔社
- 許浚, "東醫寶鑑", pp.1984~ (1999), 서울:法人文化社
- 尹吉榮, "東醫臨床方劑學", pp.83~ (1985), 서울:明寶出版社
- 박은희, "조각자 물추출물의 항염증 작용", 약학회지, 37(2), pp.124~8 (1993)
- 권두한, "항바이러스 활성 식물자원 탐색", 한국약용작물학회지, 11(1), pp.24~30 (2003), 한국약용작물학회
- 朴涌基, "皂角刺의 抗癌作用에 대한 實驗的 硏究", 대한본초학회지, 12(1), pp.53~66 (1997)
- 朴涌基, "皂角刺의 消炎作用에 대한 實驗的 硏究", 韓醫學硏究所論文集 4券, 3, pp.237~65 (1995)
- 안봉전, "선방활명음의 항암 및 항산화효과 검증", 대한본초학회지, 20(3), pp.51~58 (2005), 대한본초학회
- Schuchter LM, "Primary and metastatic diseases in malignant melanoma of gastrointestinal tract", Curr Opin Oncol, 12, pp.181~185 (2000)
- 문구, "암 동서의 결합치료", pp.93~7 (1999), 원광대학교출판국:신아출판사
- Choi HJ, "Petrotetrayndiol A induces cell cycle arrest and apoptosis in SK-MEL-2 human melanoma cells through cytochrome c-mediated activation of caspases", Cancer Lett, 232(2), pp.214~25 (2006)
- N. Iizuka, "Comparison of gene expression profiles between hepatitis B virus- and hepatitis C virus-infected hepatocellular carcinoma by oligonucleotide microarray data on the basis of a supervised learning method", Cancer Res, 62, pp.3939~44 (2002)
- Hong Y, "A susceptibility gene set for early onset colorectal cancer that integrates diverse signaling pathways: implication for tumorigenesis", Clinical Cancer Research, 13(4), pp.1107~14 (2007)
- Galbraith DW, "The daunting process of MIAME", Nature, 444(7115), pp.31~ (2006)
- Roslev P, "Application of a Tetrazolium Salt with a Water-Soluble Formazan as an Indicator of Viability in Respiring Bacteria", Appl Environ Microbiol, 59(9), pp.2891~6 (1993)
- Florell SR, "Preservation of RNA for functional genomic studies. a multidisciplinary tumor bank protocol", Modern Pathology, 14(2), pp.116~28 (2001)
- Weis S, "Quality control for microarray analysis of human brain samples: The impact of postmortem factors, RNA characteristics, and histopathology", Journals of Neuroscience Methods, 165(2), pp.198~209 (2007)
- Kwon KH, "Analysis of gene expression profiles in cholesteatoma using oligonucleotide microarray", Acta oto-laryngologica, 126(7), pp.691~7 (2006)
- Zahurak M, "Pre-processing Agilent microarray data", BMC Bioinformatics, 8, pp.142~ (2007)
- Pavlícek A, "Free- Tree--freeware program for construction of phylogenetic trees on the basis of distance data and bootstrap", jackknife analysis of the tree robustness. Application in the RAPD analysis of genus Frenkelia.Folia Biol (Praha), 1999, pp.45(3), 97~9
- 李時珍, "本草綱目 3券", pp.2021~2 (1993), 서울:醫聖堂
- 朴涌基, "조각자나무의 부위 및 채취시기별 Saponin 함량차이에 관한 연구", pp.1990
- 김충희, "랫드에서 조각자(주엽) 나무 추출물인 gleditschia saponin의 경구 2주 반복투여 독성실험", 한국독성학회, 18(3), pp.285~92 (2002)
- 송상진, "조각자가 천식모델 생쥐의 면역세포 및 사이토카인에 미치는 영향", pp.2005
- 박경덕, "피부 악성 흑색종의 임상 및 병리조직학적 소견", 대한피부과학회지, 45(2), pp.149~158 (2007), 대한피부과학회
- 조용찬, "불명확한 원발부위의 전이성 대장 악성흑색종 1예", 대한소화기내시경학회지, 32(1), pp.62~66 (2006), 대한소화기내시경학회
- 한진근, "楡白皮 추출물이 인간의 피부 세포 재생 및 악성 흑색종 세포에 미치는 영향", 박사, pp.2007
- 고홍개, "化氣調經湯이 피부 세포 재생 및 악성 흑색종 세포에 미치는 영향", 박사, pp.2007
- Blanchard AP, "High-density oligonucleotide arrays", Biosensors Bioelectron, 11, pp.687~90 (1996)
- Hughes TR, "Expression profiling using microarrays fabricated by an ink-jet oligonucleotide synthesizer", Nature Biotechnology, 19, pp.342~7 (2001)
- Dewji N, "Molecular cloning of the sphingolipid activator protein-1 (SAP-1). the sulfatide sulfatase activator", Biochem Biophys Research Commun, 134(2), pp.989~94 (1986)
- Giles RH, "Construction of a 1.2-Mb contig surrounding, and molecular analysis of the human CREB-binding protein (CBP", CREBBP) gene on chromosome, 1997, pp.42(1), 96~114
- Miller JC, "Restraint stress influences AP-1 and CREB DNAbinding activity induced by chronic lithium treatment in the rat frontal cortex and hippocampus", The International Tournal of Neuropsychopharmacology, 10(5), pp.609~19 (2007)
- Kriehuber E, "Balance between NF-κB and JNK", AP-1 activity controls dendritic cell life and death, 2005, pp.106(1), 175~83