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側柏葉이 인간 유래 악성 흑색종 세포의 유전자 발현에 미치는 영향

Effects of Thujae Orientalis Folium (TOF) on Gene Expression of Human melanoma cells (SK-MEL-2)

최정화|김종한|박수연|정민영
대한한방안이비인후피부과학회 (The Korean Medicine Opthalmology&Otolaryngology&Dermatology Society)|2010.08|한방안이비인후피부과학회지 (The Journal of Korean Medicine Ophthalmology&Otorhinolaryngology&Dermatology)|제23권 제2호|pp. 81-108

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키워드

anti-cancerThujae Orientalis Folium (TOF)anti-tumor componenttyrosinase inhibitory effectwhitening activitieshuman melanoma.
주제분류한의학

초록

Objecitve : Thujae Orientalis Folium (TOF) can cool the blood and stop bleeding, eliminate phlegm and relieve cough in Oriental medicine. In addition, the fresh is used alone externally. Recently, TOF is known to have anti-tumor component. And also known to have tyrosinase inhibitory effect. Method : For these reasons, this study was designed to investigate anti-cancer and whitening activities of TOF. In this experiment, effects of TOF on proliferation rates of melanoma cells and on changes in genetic profiles were investigated. The genetic profile for the effect on human derived melanoma cell, SK-MEL-2,was measured using microarray technique, and the functional analysis on these genes was conducted. Results : Total 541 genes were up-regulated and 1,079 genes down-regulated in cells treated with TOF. Genes induced by TOF were mainly concerned with anti-cancer effects and apoptosis. Genes suppresed by TOF were related in extracellular signalling pathway. The network of total protein interactions was measured using cytoscape program, and some key molecules, such as THAP1, MAX1, STAM2, SMAD6, CYCS, PEX5,PSEN1, NONO, MAP2K7 and CREB1 that can be used for elucidation of therapeutical mechanism of medicine in future were identified. Conculusion : These results suggest possibility of TOF as anti-cancer drug for human melanoma. In addition, the present author also suggest that related mechanisms are involved in inhibition of several cancer pathway, activation of apoptosis pathway and suppression of general metabolic pathway.

Abstract

Objecitve : Thujae Orientalis Folium (TOF) can cool the blood and stop bleeding, eliminate phlegm and relieve cough in Oriental medicine. In addition, the fresh is used alone externally. Recently, TOF is known to have anti-tumor component. And also known to have tyrosinase inhibitory effect. Method : For these reasons, this study was designed to investigate anti-cancer and whitening activities of TOF. In this experiment, effects of TOF on proliferation rates of melanoma cells and on changes in genetic profiles were investigated. The genetic profile for the effect on human derived melanoma cell, SK-MEL-2,was measured using microarray technique, and the functional analysis on these genes was conducted. Results : Total 541 genes were up-regulated and 1,079 genes down-regulated in cells treated with TOF. Genes induced by TOF were mainly concerned with anti-cancer effects and apoptosis. Genes suppresed by TOF were related in extracellular signalling pathway. The network of total protein interactions was measured using cytoscape program, and some key molecules, such as THAP1, MAX1, STAM2, SMAD6, CYCS, PEX5,PSEN1, NONO, MAP2K7 and CREB1 that can be used for elucidation of therapeutical mechanism of medicine in future were identified. Conculusion : These results suggest possibility of TOF as anti-cancer drug for human melanoma. In addition, the present author also suggest that related mechanisms are involved in inhibition of several cancer pathway, activation of apoptosis pathway and suppression of general metabolic pathway.

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忍冬藤이 인간 유래 악성 흑색종 세포의 유전자 발현에 미치는 영향강성인, 김대수, 김종한, 박수연, 최정화대한한방안이비인후피부과학회 · 한방안이비인후피부과학회지2009 · 제22권 제1호DNA chip을 이용한 조각자 추출물의 인간유래 악성 종양에 미치는 영향김종한, 박수연, 박용호, 최정화대한한방안이비인후피부과학회 · 한방안이비인후피부과학회지2008 · 제21권 제1호皂角刺가 악성흑색종 세포주에 미치는 영향김부여, 김용성, 이병호, 임세현, 임지연, 조수인대한한방안이비인후피부과학회 · 한방안이비인후피부과학회지2009 · 제22권 제3호측백나무 추출물의 Tyrosinase 활성저해 및 Melanin 생성 저해모정희, 오수정한국피부과학연구원 · 아시안뷰티화장품학술지2015 · 제13권 제2호皂角刺 추출물이 인간 유래 유방암 세포의 유전자 발현에 미치는 영향박경미, 반혜란, 양승정, 조성희대한한방부인과학회 · 대한한방부인과학회지2009 · 제22권 제2호황금추출물이 인간 유래 자궁경부암세포의 유전자발현에 미치는 영향구희준, 박경미, 양승정, 조성희, 조현정대한한방부인과학회 · 대한한방부인과학회지2009 · 제22권 제3호

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