Investigation of -308G>A and -1031T>C Polymorphisms in the TNFA Promoter Region in Polish Peptic Ulcer Patients
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Background/Aims: Tumor necrosis factor α (TNF-α) encod-ed by TNFA is a key mediator in inflammation, a precursor condition for peptic ulceration. Promoter polymorphisms of TNFA that influence its transcriptional activity and TNF-α pro-duction are known. TNFA-308G>A (rs1800629) and TNFA-1031T>C (rs1799964), which are responsible for increased TNFA transcription, could influence the risk of peptic ulcer-ation. This study aimed to investigate these polymorphisms and to evaluate their association with peptic ulcer disease and Helicobacter pylori infection in the Polish population. Methods: Gastric mucosa specimens obtained from 177 Polish peptic ulcer patients were used to conduct rapid ure-ase tests and to assess the investigated polymorphisms by polymerase chain reaction-restriction fragment length poly-morphism. Genotyping data were compared with the results obtained from healthy individuals of Polish origin. Results: There were no significant differences in genotype and allele frequency of the investigated polymorphisms between pep-tic ulcer patients and healthy individuals. No associations between the frequencies of particular genotypes and alleles for both single-nucleotide polymorphisms (SNPs) and the presence of H. pylori infection in peptic ulcer patients and in subgroups of men and women with peptic ulcer disease were found. Conclusions: The investigated SNPs are not risk factors for either peptic ulcer or H. pylori infection develop-ment in the Polish population. The results require verification in a larger cohort.
Abstract
Background/Aims: Tumor necrosis factor α (TNF-α) encod-ed by TNFA is a key mediator in inflammation, a precursor condition for peptic ulceration. Promoter polymorphisms of TNFA that influence its transcriptional activity and TNF-α pro-duction are known. TNFA-308G>A (rs1800629) and TNFA-1031T>C (rs1799964), which are responsible for increased TNFA transcription, could influence the risk of peptic ulcer-ation. This study aimed to investigate these polymorphisms and to evaluate their association with peptic ulcer disease and Helicobacter pylori infection in the Polish population. Methods: Gastric mucosa specimens obtained from 177 Polish peptic ulcer patients were used to conduct rapid ure-ase tests and to assess the investigated polymorphisms by polymerase chain reaction-restriction fragment length poly-morphism. Genotyping data were compared with the results obtained from healthy individuals of Polish origin. Results: There were no significant differences in genotype and allele frequency of the investigated polymorphisms between pep-tic ulcer patients and healthy individuals. No associations between the frequencies of particular genotypes and alleles for both single-nucleotide polymorphisms (SNPs) and the presence of H. pylori infection in peptic ulcer patients and in subgroups of men and women with peptic ulcer disease were found. Conclusions: The investigated SNPs are not risk factors for either peptic ulcer or H. pylori infection develop-ment in the Polish population. The results require verification in a larger cohort.
참고문헌 (26)
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