TNF, IL12B, and IFNG Gene Polymorphisms in Serbian Patients with Psoriasis
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Background: Psoriasis is a common chronic inflammatoryskin disease with a strong genetic basis. Cytokines such as tumornecrosis factor alpha (TNF-α), interleukins (ILs) such areIL-12 and IL-23, and interferon gamma (IFN-γ) are releasedfrom various inflammatory and resident cells, and have beenimplicated in the initiation/maintenance of inflammation. Certain alleles of the aforementioned cytokines may be associatedwith disease susceptibility/severity. Objective: To investigatethe association of three common functional genepolymorphisms, namely TNF −308 G/A (rs1800629), IL12B(encoding the p40 subunit of IL-12/23) ＋1188 A/C (rs3212227),and IFNG ＋874 T/A (rs2430561) with psoriasis developmentand severity in Serbian patients. Methods: We genotyped130 patients with psoriasis (26 of whom also had psoriaticarthritis) and 259 controls; rs1800629 and rs3212227,and rs2430561, by real-time PCR assay. Results: The TNFGG genotype was detected at a higher frequency in patientswith psoriasis compared to control subjects (OR, 1.420; 95%CI, 0.870∼2.403) without statistical significance (p=0.191). Lack of the TNF G allele was associated with lowerpsoriasis severity (p=0.007). The IL12B AC genotype wasunderrepresented in the patients with psoriatic arthritis comparedto healthy subjects (OR, 0.308; 95% CI, 0.090∼1.057; p=0.049). The distribution of the rs2430561 alleleand genotype frequencies was similar between patients withpsoriasis and controls. Conclusion: Our study demonstratesan effect of the rs1800629 on psoriasis severity, and a marginalimpact of the rs3212227 on susceptibility to psoriaticarthritis. Collectively, our results obtained in a Serbian cohortexpand current knowledge regarding individual predispositionto psoriatic disease.
Abstract
Background: Psoriasis is a common chronic inflammatoryskin disease with a strong genetic basis. Cytokines such as tumornecrosis factor alpha (TNF-α), interleukins (ILs) such areIL-12 and IL-23, and interferon gamma (IFN-γ) are releasedfrom various inflammatory and resident cells, and have beenimplicated in the initiation/maintenance of inflammation. Certain alleles of the aforementioned cytokines may be associatedwith disease susceptibility/severity. Objective: To investigatethe association of three common functional genepolymorphisms, namely TNF −308 G/A (rs1800629), IL12B(encoding the p40 subunit of IL-12/23) ＋1188 A/C (rs3212227),and IFNG ＋874 T/A (rs2430561) with psoriasis developmentand severity in Serbian patients. Methods: We genotyped130 patients with psoriasis (26 of whom also had psoriaticarthritis) and 259 controls; rs1800629 and rs3212227,and rs2430561, by real-time PCR assay. Results: The TNFGG genotype was detected at a higher frequency in patientswith psoriasis compared to control subjects (OR, 1.420; 95%CI, 0.870∼2.403) without statistical significance (p=0.191). Lack of the TNF G allele was associated with lowerpsoriasis severity (p=0.007). The IL12B AC genotype wasunderrepresented in the patients with psoriatic arthritis comparedto healthy subjects (OR, 0.308; 95% CI, 0.090∼1.057; p=0.049). The distribution of the rs2430561 alleleand genotype frequencies was similar between patients withpsoriasis and controls. Conclusion: Our study demonstratesan effect of the rs1800629 on psoriasis severity, and a marginalimpact of the rs3212227 on susceptibility to psoriaticarthritis. Collectively, our results obtained in a Serbian cohortexpand current knowledge regarding individual predispositionto psoriatic disease.
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