아토피양 접촉피부염 유발 NC/Nga mouse에서 도고 온천수에 첨가한 게르마늄 성분의 효과
Effect of DOGO phreatic water containing germanium on Atopic Dermatitis in NC/Nga mouse
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Objectives : The purpose of this study is to investigate the effects of DOGO phreatic water containinggermanium on Atopic Dermatitis in NC/Nga mouse. Methods : We made DOGO phreatic water added germanium. After making atopic dermatitis caused bysensitizing NC/Nga mouse to DNCB(dinitrochlorobenzene), we made mouse swim in tanks each filled withdistilled water, tap water, DOGO phreatic water, DOGO phreatic water(added germanium) for 30minutes everyday. 3weeks later, we analyzed skin clinical score, total IgE levels(by ELISA), WBC differential counting(Neutrophils,Monocytes), absolute cell number of Neutrophil+Gr-1+, CCR3 mRNA expressions(by Real-time PCR), IL-4, IFN-Ɣproduction levels(by ELISA), histologic test(by H&E staining, toluidine blue staining). Results : The results of making NC/Nga mouse induced atopic dermatitis swim in tanks filled with DOGOphreatic water(contain germanium) are as follows. 1. Skin clinical scores were decreased significantly in comparison to control group. 2. Total IgG levels were decreased significantly in comparison to control group. 3. WBC differential counting(Neutrophils, Monocytes) were decreased significantly in comparison tocontrol group. 4. Absolute cell number of Neutrophil+Gr-1+ were decreased significantly in comparison to controlgroup. 5. CCR3 mRNA expressions were decreased significantly in comparison to control group. 6. IL-4, IFN-Ɣ production levels were decreased significantly in comparison to control group. 7. The epithelial tissue thickness, leucocytes infiltration, erythema, edema, excoriation, scaling, mastcells infiltrations in dorsal skin were decreased in comparison to control group. Conclusions : These results indicate that DOGO phreatic water(contain germanium) can be used for helping treatatopic dermatitis.
Abstract
Objectives : The purpose of this study is to investigate the effects of DOGO phreatic water containinggermanium on Atopic Dermatitis in NC/Nga mouse. Methods : We made DOGO phreatic water added germanium. After making atopic dermatitis caused bysensitizing NC/Nga mouse to DNCB(dinitrochlorobenzene), we made mouse swim in tanks each filled withdistilled water, tap water, DOGO phreatic water, DOGO phreatic water(added germanium) for 30minutes everyday. 3weeks later, we analyzed skin clinical score, total IgE levels(by ELISA), WBC differential counting(Neutrophils,Monocytes), absolute cell number of Neutrophil+Gr-1+, CCR3 mRNA expressions(by Real-time PCR), IL-4, IFN-Ɣproduction levels(by ELISA), histologic test(by H&E staining, toluidine blue staining). Results : The results of making NC/Nga mouse induced atopic dermatitis swim in tanks filled with DOGOphreatic water(contain germanium) are as follows. 1. Skin clinical scores were decreased significantly in comparison to control group. 2. Total IgG levels were decreased significantly in comparison to control group. 3. WBC differential counting(Neutrophils, Monocytes) were decreased significantly in comparison tocontrol group. 4. Absolute cell number of Neutrophil+Gr-1+ were decreased significantly in comparison to controlgroup. 5. CCR3 mRNA expressions were decreased significantly in comparison to control group. 6. IL-4, IFN-Ɣ production levels were decreased significantly in comparison to control group. 7. The epithelial tissue thickness, leucocytes infiltration, erythema, edema, excoriation, scaling, mastcells infiltrations in dorsal skin were decreased in comparison to control group. Conclusions : These results indicate that DOGO phreatic water(contain germanium) can be used for helping treatatopic dermatitis.
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