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凉血除濕湯이 DNCB로 유도된 NC/Nga mice의 아토피 피부염에 미치는 영향

Effect of Yanghyeoljeseuptang (YHJST) Composition on Atopic Dermatitis NC/Nga Mice Induced by DNCB(dinitrochlorobenzene)

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초록

Objectives The purpose of this study is to investigate the effect of YHJST on atopic dermatitis in an experiment using an NC/Nga mice induced by DNCB, which has histological and clinical similarities to the condition in humans. Methods To investigate the effect of YHJST on atopic dermatitis(AD), we evaluated atopic dermatitis-like skin lesions by clinical skin index and analyzed immunological parameters in peripheral blood mononuclear cells(PBMCs) and performed skin histology in ears and dorsal skin of NC/Nga ato-mouse. Results YHJST medicines decreased Serum level of IgE, IL-6, TNF-α. Also total number of CD69+, CD3+ in PBMCs, absolute cell number of CCR3+CD3+, CD11b+Gr-1+ in Dorsal skin tissue, Serum IgG1, IgM, IgG2a and IgG2b decreased significantly. Furthermore YHJST is extremely effective to histological symptoms; dermal and epidermal thickening, hyperkeratosis and inflammatory cell infiltration and suppressed histologic infiltration of CD4+& CCR3+ in ear and dorsal skin lesions significantly. YHJST decreased gene-expression of IL-6, TNF-α, CCR3, Eotaxin mRNA than that of control group. Conclusions YHJST on atopic dermatitis to atopic dermatitis NC/Nga mouse induced DNCB was incredibly effective.

Abstract

Objectives The purpose of this study is to investigate the effect of YHJST on atopic dermatitis in an experiment using an NC/Nga mice induced by DNCB, which has histological and clinical similarities to the condition in humans. Methods To investigate the effect of YHJST on atopic dermatitis(AD), we evaluated atopic dermatitis-like skin lesions by clinical skin index and analyzed immunological parameters in peripheral blood mononuclear cells(PBMCs) and performed skin histology in ears and dorsal skin of NC/Nga ato-mouse. Results YHJST medicines decreased Serum level of IgE, IL-6, TNF-α. Also total number of CD69+, CD3+ in PBMCs, absolute cell number of CCR3+CD3+, CD11b+Gr-1+ in Dorsal skin tissue, Serum IgG1, IgM, IgG2a and IgG2b decreased significantly. Furthermore YHJST is extremely effective to histological symptoms; dermal and epidermal thickening, hyperkeratosis and inflammatory cell infiltration and suppressed histologic infiltration of CD4+& CCR3+ in ear and dorsal skin lesions significantly. YHJST decreased gene-expression of IL-6, TNF-α, CCR3, Eotaxin mRNA than that of control group. Conclusions YHJST on atopic dermatitis to atopic dermatitis NC/Nga mouse induced DNCB was incredibly effective.

참고문헌 (113)

  1. Morren MA, "Atopic dermatitis : triggering factors", J Am Acad Dermatol, pp.4473~4670 (1994)
  2. Leung DY, "Atopic dermatitis, In. Fitzpatricks dermatology in general medicine. 6th ed", pp.1180~1194 (2003), McGraw-Hill
  3. Leung DY, "Celluar and immunologic mechanisms in atopic dermatitis", J Am Acad Dermatol, 44, pp.S1~S1201 (2001)
  4. Spergel JM, "Roles of Th1 and Th2 cytokines in a murine model of allergic dermatitis", J Clin Invest, 103(8), pp.1103~1111 (1999)
  5. 洪元植, "精校黃帝內經素問", pp.72~80 (1985), 東洋醫學硏究院 出版部
  6. 박민철, "아토피 피부염의 동, 서의학적 문헌고찰", 대한안이비인후피부과학학회지, 15(1), pp.226~252 (2002)
  7. 馬紹堯, "現代中醫皮膚性病學", pp.153~160 (2001), 上海中醫藥大學出版社
  8. 金起鳳, "中醫皮膚病學", pp.193~198 (1999), 中國醫藥科技出版社
  9. 馬紹堯, "代中醫皮膚性病診療大全", pp.475~493 (1999), 山西科學技術出版社
  10. Oranje AP, "Atopic dermatitis. review 2000 to January 2001", Curr Opin Pediatr, 14, pp.410~413 (2002)
  11. 안성구, "COMMON SKIN DISEASE 진단", 치료, 2003, 68~88
  12. Marks R, "The prevalence of common skin conditions in Australian school students : 2. Atopic dermatitis", Br J Dermatol, 140, pp.468~473 (1999)
  13. 박건수, "Cytokine patterns of acute and remission stages in patients with atopic dermatitis : expression of interferon-γ, interleukin- 12", 카톨릭 대학 의학부 논문집, 51, pp.384~ (1998)
  14. 박영민, "피부과 외래환자에서 아토피 피부염의 상대빈도와 임상적 특징 및 검사 소견에 관한 연구", 대한피부과학회지, 35, pp.96~ (1997)
  15. Ring J, "The “Atopy patch test” in evaluating the role of aeroallergens in atopic eczema", Int Arch Allergy Immunol, 13, pp.379~ (1997)
  16. 장호선, "아토피 피부염의 진단과 치료", 가정의학회지, 23(7), pp.2~840 (2002), 대한가정의학회
  17. Leung DY, "Atopic dermatitis", Lancet, 361, pp.151~160 (2003)
  18. 오재원, "1995년과 2000년의 학동기와 2003년 학동전기 소아에서의 아토피피부염의 역학적 변화에 관한 전국적인 연구", 대한소아알레르기및호흡기학회지, 13(4), pp.227~237 (2003), 대한 소아알레르기 호흡기학회
  19. Abdel-Hamid KM, "Atopic dermatitis in Allergy, Asthma, and Immunology Subspecialty Consult", pp.66~73 (2003), LippincottWilliams & Wilins
  20. Boguniewicz M, "Atopic dermatitis and contact dermatitis in Manual of Allergy and Immunology", LippincottWilliams & Wilins, pp.165~186 (2002)
  21. Caron G, "Histamine polarizeshuman dendritic cells into Th2 cell- promoting effectordendritic cells", J Immunol, 167, pp.3682~3686 (2001)
  22. Cumberbatch M, "Kimber. Impact of cutaneous IL-10 on resident epidermal Langerhans’ cells and the development of polarized immuneresponses", J Immunol, 175, pp.43~50 (2005)
  23. Friedmann PS, "Contact sensitization and allergic contact dermatitis: Immunobiological mechanisms", Toxicol Lett, 162, pp.49~54 (2006)
  24. Goutet M, "Identification of contact and respiratory sensitizersusing flow cytometry", Toxicol Appl Pharmacol, 205, pp.259~270 (2005)
  25. Heo Y, "Lead differentially modifies cytokine production in vitro and in vivo", Toxicol Appl Pharmacol, 138, pp.149~157 (1996)
  26. Hilkens CM, "Human dendritic cells require exogenous interleukin-12-inducing factors to direct the development of naive T-helper cells toward the Th1 phenotype", Blood, 90, pp.1920~1926 (1997)
  27. Kepron MR, "IL-4 induced the specific rearrangement of gamma 1 genes on the expressed and unexpressed chromosomesof lipopolysaccharide- activated normal murine B cells", J Immunol, 143, pp.334~339 (1989)
  28. Kiekens RC, "Heterogeneity within tissue- specific macrophage and dendritic cell populations during cutaneous inflammation in atopic dermatitis", Br J Dermatol, 145(6), pp.957~965 (2001)
  29. Kitagaki H, "Repeated elicitation of contact hypersensitivity induces a shift in cutaneous cytokine milieu from a T helper cell type 1 to a T helper cell type 2 profile", J Immunol, 159, pp.2484~2481 (1997)
  30. Leung DYM, "Atopic dermatitis in Fitzpatrick’s Dermatology in General Medicine", pp.1464~1480 (1999), McGraw-Hill
  31. Matsumoto M, "IgE hyperproduction through enhanced tyrosine phosphorylation of Janus kinase 3 in NC", Nga mice, a model for human atopic dermatitis, 1999, pp.162, 1056~1063
  32. Mueller DL, "Clonal expansion versus functinal clonal inactivation : A costimulatory signalling pathway determines the outcome of T cell antigen receptor occupancy", Ann Rev Immunol, 7, pp.445~480 (1989)
  33. Ohmen JD, "Overexpression of IL-10 in atopic dermatitis", J Immunol, 154, pp.1956~1963 (1995)
  34. Plitnick LM, "Cytokine profiling for chemical sensitizers: Application of the ribonuclease protection assay and effect of dose", Toxicol Appl Pharmacol, 179, pp.145~154 (2002)
  35. Sikorski EE, "Phenotypic analysis of lymphocyte subpopulations in lymph nodes draining the ear following exposure to contact allergens and irritants", Fund Appl Toxicol, 34, pp.25~35 (1996)
  36. Snapper CM, "Interferon-γ and B cell stimulatory factor-1 reciprocally regulate Ig isotype production", Science, 236, pp.944~947 (1987)
  37. Tsunemi Y, "Interleukin-13 gene polymorphism G4257A is associated with atopic dermatitis in Japanese patients", J Dermatol Sci, 30, pp.100~107 (2002)
  38. US Dept, "Health and Human Services. Atopic dermatitis (A type of eczema)", pp.4272~ (2003), NIH publication
  39. Wiemann C, "Joint positive control testing in guinea pig skin sensitization tests", Reg Toxicol Pharmacol, 35, pp.14~22 (2002)
  40. Wills-Karp M, "Interleukin-13 : Central mediator of allergic asthma", Science, 282, pp.2258~2261 (1998)
  41. Zhu Z, "Pulmonary expression of interleukin-13 causes inflmmation, mucus hypersecretion, subepithelial fibrosis, physiologic abnormalities, and eotaxin production", J Clincal Invest, 103, pp.779~789 (1999)
  42. Zimmermann N, "Chemokines in asthma : Cooperative interaction between chemokines and IL-13", J Allergy Clin Immunol, 111, pp.227~242 (2003)
  43. Yang G, "Anti-IL-13 monoclonal antibody inhibits airway hyperresponsiveness, inflammation and airway remodeling", Cytokine, 28, pp.224~232 (2004)
  44. Walter DM, "Critical role for IL-13 in the development of allergen-induced airway hyperreactivity", J Immunol, 167, pp.4668~4675 (2002)
  45. 정은미, "학동기 천식 환아의 약 10년간 추적 관찰 후 기관지 과민도의 변화 및 이들 변화에 미치는 요소에 관한 연구", Allergy Asthma & Respiratory Diseases, 12(2), pp.82~92 (2002), 대한 소아알레르기 호흡기학회
  46. Yang M, "Eotaxin- 2 and IL-5 cooperate in the lung to regulate IL-13 production and airway eosinophilia and hyperreactivity", J Allergy Clin Immunol, 112, pp.935~943 (2003)
  47. Li L, "Effect of Th2 cytokines on chemokine expression in the lung : IL-13 potently induces eotaxin expression by airway epithelial cells", J Immunol, 162, pp.2477~2487 (1999)
  48. Pope SM, "IL-13 induces eosinophil recruitment into the lung by an IL-5 and eotaxin dependent mechanism", J Allergy Clin Immunol, 108, pp.594~601 (2001)
  49. Matsukura S, "Interleukin-13 upregulates eotaxin expression in airway epithelial cells by a STAT6-dependent mechanism", Am J Respir Cell Mol Biol, 24, pp.755~761 (2001)
  50. Moore PE, "IL-13 and IL-4 cause eotaxin release in human airway smooth muscle cells : a role for ERK", Am J Physiol Lung Cell Mol Physiol, 282, pp.847~852 (2002)
  51. Schmid-Grendelmeier P, "Eosinophils express functional IL-13, in eosinophilic inflammatory disease", J Immunol, 169, pp.1021~1027 (2002)
  52. 김진택, "소아천식 기도염증의 비침습적 평가방법", Korean Journal of Pediatrics, 44(3), pp.334~339 (2001), 대한소아과학회
  53. Arima K, "Upregulation of IL-13 concentraion in vivo the IL-13 variant associated with bronchial asthma", J Allergy Clin Immunol, 109, pp.980~987 (2002)
  54. 巢元方, "巢氏諸病源候論", 대성문화사, pp.251~253 (1992)
  55. 陳實功, "外科正宗", pp.298~ (2000), 天津科學技術出版社
  56. 肅達民, "兒科病", 人民衛生出版社, pp.664~674 (2002)
  57. Abeck D, "Optimal management of atopic dermatitis", Am Clin Dermatol, 1, pp.41~46 (2000)
  58. -Brown RA, "Therapeutic in atopic dermatitis", Adv Dermatol Clin, 8, pp.747~750 (1990)
  59. 이지현, "아토피피부염학회 보고서: 한국 아토피피부염의 치료지침", 대한피부과학회지, 44(8), pp.907~913 (2006), 대한피부과학회
  60. 이진용, "아토피피부염의 한방치료", 대한한방알레르기 및 면역학회 자료집, pp.61~79 (2002)
  61. 전국한의과대학, "本草學", pp.160~163 (2000), 도서출판 永林社
  62. 임철홍, "白鮮皮가 항알레르기의 작용에 미치는 영향", 석사, pp.1994
  63. 이석원, "牧丹皮 추출물 및 EA분획의 항알러지 작용에 대한 연구", 동의병리학회지, 15(4), pp.631~635 (2001)
  64. 신광식, "生地黃 추출물이 DNCB로 유도된 생쥐의 allergy성 접촉피부염에 미치는 영향", 석사, pp.1999
  65. 이용숙, "金銀花-黃芩 配合이 항염증 작용에 미치는 영향", 박사, pp.2005
  66. 정선영, "苦蔘의 생쥐의 B-cells에 미치는 항알레르기 효과", 석사, pp.2002
  67. 우무륜, "苦蔘이 알러지성 염증 반응에 미치는 영향", 대전대학교 한의학연구소 논문집, 15(2), pp.135~148 (2006)
  68. O'Bryan L, "Localized eosinophil degranulation mediates disease in tropical pulmonary eosinophilia", Infect Immun, 71, pp.1337~1342 (2003)
  69. Min JW, "Comparison of plasma eotaxin family level in aspirininduced and aspirin- tolerant asthma patient", Chest, 128, pp.127~132 (2005)
  70. Li J, "Haemopoietic mechanisms in murine allergic upper and lower airway inflammation", Immunology, 114, pp.386~396 (2005)
  71. Taube C, "The role of IL-13 in established allergic airway disease", J Immunol, 169, pp.6482~6489 (2002)
  72. Venkayya R, "The Th2 lymphocyte products IL-4 and IL-13 rapidly induce airway hyperresponsiveness through direct effects on resident airway cells", Am J Respir Cell Mol Biol, 26, pp.202~208 (2002)
  73. 장지영, "경증 천식 환아의 기관지과민성 유발 관여 요소", 대한소아알레르기및호흡기학회지, 15(2), pp.142~149 (2005), 대한 소아알레르기 호흡기학회
  74. Shin SA, "Relationship between airway hyperresposiveness and duration of asthma in children", J Asthma Allergy Clin Immunol, 23, pp.77~83
  75. Hummelshoj T, "Association between an interleukin-13 promotor polymorphism and atopy", Eur J Immunogenet, 30, pp.355~359 (2003)
  76. Zhou G, "Haplotype structure and evidence for positive selection at the human IL13 locus", Mol Biol Evol, 21, pp.29~35 (2004)
  77. Baggiolini M, "Human chemokines", an update, Annu Rev Immunol, 15, pp.675~705 (1997)
  78. Bartels J, "Human dermal fibroblasts expression eotaxin: molecular cloning mRNA expression, and identification of eotaxin sequence variants", Biochem Biophys Res Commun, 225, pp.1045~1051 (1996)
  79. Bonecchi R, "Differential expression of chemokine receptors and chemotactic responsiveness of type 1 T helper cells (Th1s) and Th2s", J Exp Med, 187, pp.129~134 (1998)
  80. Daugherty BL, "Cloning expression and characterization of the human eosinophil eotaxin receptor", J Exp Med, 183, pp.2349~2354 (1996)
  81. Garcia-Zepeda EA, "Human eotoxin is a specific chemoattractant for eosinophil cells and provides a new mechanism to explain tissue eosinophilia", Nat Med, 2, pp.449~456 (1996)
  82. Gerber BO, "Functional expression of the eotaxin receptor CCR3 in T lymphocytes co-localizing with eosinophils", Curr Biol, 7, pp.836~843 (1997)
  83. Grewe M, "A role for Th1 and Th2 cells in the immunopathogenesis of atopic dermatitis", Immunol Today, 19, pp.359~361 (1998)
  84. Grewe M, "Lesional expression of interferon-È in atopic eczema", Lancet, 343, pp.24~26 (1994)
  85. Hamid Q, "Differential in situ cytokine gene expression in acute versus chronic atopic dermatitis", J Clin Invest, 94, pp.870~876 (1994)
  86. Hanifin J, "Diagnostic features of atopic dermatitis", Acta Dermatol Venereol, 92, pp.44~47 (1980)
  87. Harrison PR, "Molecular mechanisms involved in the regulation of gene expression during cell differentiation and development", Immunol Ser, 49, pp.411~464 (1990)
  88. van der Heijden FL, "High frequency of IL-4- producing CD4+ allergen-specific T lymphocytes in atopic dermatitis lesional skin", J Invest Dermatol, 97, pp.389~394 (1991)
  89. Kitaura M, "Molecular cloning of human eotaxin, an eosinophil-selective CC chemokine, and identification of a specific eosinophil eotaxin receptor, CC chemokine receptor 3", J Biol Chem, 271, pp.7725~7730 (1996)
  90. Kunz B, "Clinical validation and guidelines for the SCORAD index: consensus report of the European Task Force on Atopic Dermatitis", Dermatology, 195, pp.10~19 (1997)
  91. Lamkhioued B, "Increased expression of eotaxin in bronchoalveolar lavage and airways of asthmatics contributes to the chemotaxis of eosinophils to the site of inflammation", J Immunol, 159, pp.4593~4601 (1997)
  92. Leiferman KM, "The role of eosinophils in atopic dermatitis. in: Atopic Dermatitis. From Pathogenesis to Treatment", pp.145~172 (1996), Heidelberg: Springer-Verlag
  93. Lilly CM, "Expression of eotaxin by human lung epithelial cells: induction by cytokines and inhibition by glucocorticoids", J Clin Invest, 99, pp.1767~1773 (1997)
  94. Luster AD, "Chemokine-chemotactic cytokines that mediate inflammation", New Engl J Med, 338, pp.436~445 (1998)
  95. Mattoli S, "Eotaxin expression and eosinophilic inflammation in asthma", Biochem Biophys Res Commun, 236, pp.299~301 (1997)
  96. Minshall EM, "Eotaxin mRNA and protein expression in chronic sinusitis and allergen-induced nasal responses in seasonal allergic rhinitis", Am J Respir Cell Mol Biol, 17, pp.683~690 (1997)
  97. Mochizuki M, "IL-4 induces eotaxin: a possible mechanism of selective eosinophil recruitment in helminth infection and atopy", J Immunol, 160, pp.60~68 (1998)
  98. Ohmen JD, "Overexpression of IL-10 in atopic dermatitis. Contrasting cytokine patterns with delayed- type hypersensitivity reactions", J Immunol, 154, pp.1956~1963 (1995)
  99. Ponath PD, "Molecular cloning and characterization of a human eotaxin receptor expressed selectively on eosinophils", J Exp Med, 183, pp.2437~2448 (1996)
  100. Rubbert A, "Dendritic cells express multiple chemokine receptors used as coreceptors for HIV entry", J Immunol, 160, pp.3933~3941 (1998)
  101. Uguccioni M, "High expression of the chemokine receptor CCR3 in human blood basophils. Role in activation by eotaxin, MCP-4, and other chemokines", J Clin Invest, 100, pp.1137~1143 (1997)
  102. Sallusto F, "Selective expression of the eotaxin receptor CCR3 by human T helper 2 cells", Science, 277, pp.2005~2007 (1997)
  103. van Reijsen FC, "Skin-derived aeroallergen-specific T-cell clones of Th2 phenotype in patients with atopic dermatitis", J Allergy Clin Immunol, 90, pp.184~193 (1992)
  104. Romagnani S, "Th1 and Th2 in human diseases", Clin Immunol Immunopathol, 80, pp.225~235 (1996)
  105. Ying S, "Enhanced expression of eotaxin and CCR3 mRNA and protein in atopic asthma. Association with airway hyperresponsiveness and predominant co-localization of eotaxin mRNA to bronchial epithelial and endothelial cells", Eur J Immunol, 27, pp.3507~3516 (1997)
  106. Ying S, "The kinetics of allergen-induced transcription of messenger RNA for monocyte chemotactic protein-3 and RANTES in the skin of human atopic subjects: relationship to eosinophil, T cell, and macrophage recruitment", J Exp Med, 181, pp.2153~2159 (1995)
  107. Ulfgren AK, "Detection of cytokine producing cells in the synovial membrane from patients with rheumatoid arthritis", Ann Rheumatic Diseases, 54, pp.654~661 (1995)
  108. Xia MQ, "Immunohistochemical study of the beta-chemokine receptors CCR3 and CCR5 and their ligands in normal and Alzheimers disease brains", Am J Pathol, 153, pp.3137~ (1998)
  109. Yamada H, "Eotaxin is a potent chemotaxin for human basophils", Biochem Biophys Res Commun, 231(2), pp.365~368 (1997)
  110. Bronte V, "Apoptotic death of CD8+ T lymphocytes after immunization: induction of a suppressive population of Mac-1+", Gr-1+ cells, 1998, pp.161(10), 5313~5320
  111. Bronte V, "Unopposed production of granulocyte- macrophage colony-stimulating factor by tumors inhibits CD8+ T cell responses by dysregulating antigen-presenting cell maturation", J Immunol, 162(10), pp.5728~5737 (1999)
  112. Angulo I, "Nitric oxide-producing CD11b(+)Ly-6G (Gr-1)(+)CD31(ER-MP12)(+) cells in the spleen of cyclophosphamide-treated mice: implications for T-cell responses in immunosuppressed mice", Blood, 95(1), pp.212~220 (2000)
  113. Young MR, "Suppression of T cell proliferation by tumor-induced granulocyte- macrophage progenitor cells producing transforming growth factor-b and nitric oxide", J Immunol, 156(5), pp.1916~1922 (1996)

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