양단탕 추출물의 항염증 효과에 대한 연구
Studies on Anti-inflammatory Effects of Yangdan-tang Extracts
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Yangdan-tang (YD) is recorded as a treatment to treat exterior-related fever illness in the Korean medicine. In this study, we examined the anti-inflammatory effects of YD, using YD water extract and lipopolysaccharide (LPS)-induced RAW 264.7 cells. First of all, we measured the amount of nitric oxide (NO) and prostaglandin E2 (PGE2), the products of inflammatory metabolism. Also, we measured enzymes such as inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), as well as cytokines such as tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), interleukin 1 alpha (IL-1α), and interleukin 1 beta (IL-1β). YD suppressed the production of NO and PGE2 in a dose dependent manner and reduced the amount of protein and the mRNA expression of iNOS and COX-2. Also, YD reduced the mRNA expression of TNF-α, IL-6, IL-1α and IL-1β. In conclusion, YD decreased production of LPS-induced inflammatory factor, which could be a clinical basic subject for inflammatory diseases.
Abstract
Yangdan-tang (YD) is recorded as a treatment to treat exterior-related fever illness in the Korean medicine. In this study, we examined the anti-inflammatory effects of YD, using YD water extract and lipopolysaccharide (LPS)-induced RAW 264.7 cells. First of all, we measured the amount of nitric oxide (NO) and prostaglandin E2 (PGE2), the products of inflammatory metabolism. Also, we measured enzymes such as inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), as well as cytokines such as tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), interleukin 1 alpha (IL-1α), and interleukin 1 beta (IL-1β). YD suppressed the production of NO and PGE2 in a dose dependent manner and reduced the amount of protein and the mRNA expression of iNOS and COX-2. Also, YD reduced the mRNA expression of TNF-α, IL-6, IL-1α and IL-1β. In conclusion, YD decreased production of LPS-induced inflammatory factor, which could be a clinical basic subject for inflammatory diseases.
참고문헌 (32)
- Jung HY, "Molecular inflammation as the underlying mechanism of aging process and vascular diseases", KSBMB News, 30(3), pp.41~52 (2010)
- Chung HY, "The molecular inflammatory process in aging", Antioxid Redox Signal, 8(3-4), pp.572~581 (2006)
- Li C., "Yixuerumen", pp.1122~1123 (2009), Bubin books
- Kim JB, "Interpretation of ‘Synopsis of Prescriptions of the Golden Chamber'", pp.564~ (2003), Hanuimunhwasa
- "Yangdantang B" (2007)[URL]
- Moon JJ, "Precise Interpretation of ‘Treatise on Cold Damage Diseases'", pp.71~76 (2017), Hanuimunhwasa
- "Yangdantang D" (2007)[URL]
- Heo J, "Donguibogam", pp.1069~ (2005), Donguibogam Publishing Inc
- Hwang DY, "Bangyakhappyun", pp.304~ (2003), Namsandang
- Lee TH, "Effects of Cinnamomum cassia, Cucumismelo and Thuja orientalis extract on the anti-oxidant, anti-inflammatory, anti-allergy, anti-wrinkle and whitening", pp.2008
- Lim CS, "Antioxidant Effects of P. R. ( Paeoniae Radix ) Aqua - acupuncture Solution in Vitro", Journal of Acupuncture Research, 14(2), pp.191~198 (1997)
- 윤석빈, "黃芩이 LPS로 유발된 Raw 264.7 Cells의 염증인자에 미치는 영향", 대한본초학회지, 26(2), pp.75~81 (2011), 대한본초학회
- 조수인, "黃芩의 항산화 효과", 대한본초학회지, 20(3), pp.67~74 (2005), 대한본초학회
- Lee YK, "The Experimental Study of Glycyrrhiza uralensis on Wound Healing by Antioxidant Effect", Journal of Oriental Medicine Research Institute in Daejeon University, 25(1), pp.145~153 (2016)
- 박종필, "Suppression of Inflammatory Macrophage Responese by Glycyrrhiza Uralensis Herbal Acupuncture Extract", Korean Journal of Acupuncture, 28(4), pp.49~58 (2011), 경락경혈학회
- 류혜숙, "생강 추출물 투여가 전구염증성 사이토카인 IFN-γ와 항염증성 사이토카인 IL-10 분비량에 미치는 영향", 한국식품영양학회지, 20(3), pp.259~264 (2007), 한국식품영양학회
- Kim EJ, "Antioxidative Effect of Ginger Extracts", Korean Journal of Food and Cookery Science, 9(1), pp.37~42 (1993)
- 정지은, "발효에 의한 대추의 항염증 증진 효과", 대한암예방학회지, 16(3), pp.263~268 (2011), 대한암예방학회
- 홍창의, "대추 추출물의 항산화 및 아토피 피부염 관련 항염증 효과", 생약학회지, 50(2), pp.86~95 (2019), 한국생약학회
- Yoo SR, "Gyeji-tang water extract exerts anti-inflammatory activity through inhibition of ERK and NF-κB pathways in lipopolysaccharide-stimulated RAW 264.7 cells", BMC Complement. Altern. Med, 16, pp.390~399 (2016)
- Jiao H, "Effects of guizhi tang on inflammatory cytokines in myocardial ischemia and hyperlipidemia rats", Zhongguo Zhong Yao Za Zhi, 37, pp.1634~1637 (2012)
- Kutsuwa M, "A comparative study of Kakkon-to and Keishi-to on prostaglandin E(2)release from rabbit astrocytes", Phymedicine, 5, pp.275~282 (1998)
- Kindt TJ, "Innate immunity : Tenney S. Kuby Immunology", pp.52~73 (2007), Freeman press
- 김범회, "LPS로 유도된 RAW264.7 염증모델에서 MAPK 조절에 의한 羊乳의 항염증효과", 동의생리병리학회지, 24(1), pp.80~84 (2010), 한의병리학회
- Dawson TM, "A novel neuronal messenger molecule in brain : The free radical, nitric oxide", Ann Neurol, 32, pp.297~311 (1992)
- Wang MJ, "Silymarin protects dopaminergic neurons against lipopolysaccharide-induced neurotoxicity by inhibiting microglia activation", Eur J Neurosci, 16(11), pp.2103~2112 (2002)
- Hanisch UK, "Microglia as a source and target of cytokines", Glia, 40(2), pp.140~155 (2002)
- Chen W, "Role of p38 MAP kinases and ERK in mediating ultraviolet-B induced cyclooxygenase-2 gene expression in human keratinocytes", Oncogene, 20(29), pp.3921~3926 (2001)
- Marina L, "Regulation of Cyclooxygenase 2 mRNA Stability by the Mitogen-Activated Protein Kinase p38 Signaling Cascade", Mol Cell Biol, 20(12), pp.4265~4274 (2000)
- Mitchell JA, "Cyclooxygenase-2 : regulation and relevance in inflammation", Biochemical Pharmacology, 50(10), pp.1535~1542 (1995)
- Crofford LJ, "Basic biology and clinical application of specific cyclooxygenase-2 inhibitors", Arthritis Rheum, 43(1), pp.4~13 (2000)
- Raabe T, "Relative contribution of transcription and translation to the induction of tumor necrosis factor-alpha by lipopolysaccharide", J Biol Chem, 273(2), pp.974~980 (1998)