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정향 추출물 도포가 DNCB로 유발된 알레르기성 접촉 피부염에 미치는 영향

The Effects of Syzygium aromaticum extract Spread on the Allergic Contact Dermatitis induced by DNCB

강다혜|김희택|이경엽
대한한방안이비인후피부과학회 (The Korean Medicine Opthalmology&Otolaryngology&Dermatology Society)|2013.11|한방안이비인후피부과학회지 (The Journal of Korean Medicine Ophthalmology&Otorhinolaryngology&Dermatology)|제26권 제4호|pp. 1-14

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참고문헌

키워드

SpreadhistamineAllergic Contact DermatitisSyzygium aromaticum(SAE)DNCBTh1Th2
주제분류한의학

초록

Objectives : The purpose of this study is to investigate effects of Syzygium aromaticum (SAE) spread on the allergic contact dermatitis caused by 2,4-dinitro-chlorobezene (DNCB). Methods : Forty-two mice were divided into six groups ; normal, negative control (DNCB-treated), positive control (DNCB + 1% pimecrolimus), experimental group Ⅰ, Ⅱ and Ⅲ. control and experimental groups were induced allergic contact dermatitis by DNCB. Experimental group Ⅰ(DNCB + 0.2% SAE), Ⅱ(DNCB + 1% SAE)and Ⅲ(DNCB + 5% SAE) were spread SAE and positive control was spread the 1% pimecrolimus. In this study, effect of SAE on clinical aspects on the skin, histopathological change, the blood level of IgE, cytokines,histamine were investigated. In addition, effect of SAE on spleen CD4+/CD8+ T cell subset was investigated. Results : 1. In experimental group Ⅰ, Ⅱ and Ⅲ, erythemas and edema were more reduced than negative control. 2. In experimental group Ⅰ, Ⅱ and Ⅲ inflammatory edema and the numbers of infiltrated inflammatory cells were more reduced than negative control. 3. In experimental group Ⅰ, Ⅱ and Ⅲ, clinical skin score was more reduced than negative control. 4. In experimental group Ⅱ and Ⅲ, the thickness of skin was statistically significant reduced than negative control. 5. In experimental group Ⅱ and Ⅲ, histamine release was statistically significant reduced than negative control in dose-dependantly. 6. In experimental group Ⅱ and Ⅲ, cytokines (IL-1β, TNF-α, IL-4, IL-6, IL-10) were statistically significant reduced than negative control in dose-dependantly. 7. In experimental group Ⅰ, Ⅱ and Ⅲ, the level of total IgE was statistically significant reduced than negative control in dose-dependantly. 8. In experimental group Ⅲ, CD4+ and CD8+ T cells were statistically significant decreased similar to the positive control. Conclusions : According to above experiments, Syzygium aromaticum(SAE) was effective on allergic contact dermatitis.

Abstract

Objectives : The purpose of this study is to investigate effects of Syzygium aromaticum (SAE) spread on the allergic contact dermatitis caused by 2,4-dinitro-chlorobezene (DNCB). Methods : Forty-two mice were divided into six groups ; normal, negative control (DNCB-treated), positive control (DNCB + 1% pimecrolimus), experimental group Ⅰ, Ⅱ and Ⅲ. control and experimental groups were induced allergic contact dermatitis by DNCB. Experimental group Ⅰ(DNCB + 0.2% SAE), Ⅱ(DNCB + 1% SAE)and Ⅲ(DNCB + 5% SAE) were spread SAE and positive control was spread the 1% pimecrolimus. In this study, effect of SAE on clinical aspects on the skin, histopathological change, the blood level of IgE, cytokines,histamine were investigated. In addition, effect of SAE on spleen CD4+/CD8+ T cell subset was investigated. Results : 1. In experimental group Ⅰ, Ⅱ and Ⅲ, erythemas and edema were more reduced than negative control. 2. In experimental group Ⅰ, Ⅱ and Ⅲ inflammatory edema and the numbers of infiltrated inflammatory cells were more reduced than negative control. 3. In experimental group Ⅰ, Ⅱ and Ⅲ, clinical skin score was more reduced than negative control. 4. In experimental group Ⅱ and Ⅲ, the thickness of skin was statistically significant reduced than negative control. 5. In experimental group Ⅱ and Ⅲ, histamine release was statistically significant reduced than negative control in dose-dependantly. 6. In experimental group Ⅱ and Ⅲ, cytokines (IL-1β, TNF-α, IL-4, IL-6, IL-10) were statistically significant reduced than negative control in dose-dependantly. 7. In experimental group Ⅰ, Ⅱ and Ⅲ, the level of total IgE was statistically significant reduced than negative control in dose-dependantly. 8. In experimental group Ⅲ, CD4+ and CD8+ T cells were statistically significant decreased similar to the positive control. Conclusions : According to above experiments, Syzygium aromaticum(SAE) was effective on allergic contact dermatitis.

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