山楂가 DNCB로 유발된 생쥐의 알레르기성 접촉피부염에 미치는 영향
Effects of Crataegus Pinnatifida (CP) on Allergic Contact Dermatitis (ACD) Induced by DNCB in Mice
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초록
Allergic contact dermatitis (ACD) is a type IV delayed hypersensitivity reaction that results from exposures andsubsequent sensitization to an environmental chemical. Crataegus Pinnatifida (CP) is commonly used to improvespleen function, remove retention of food, and promote blood circulation. This study is designed to investigate theeffects of CP on ACD induced by 2,4-dinitrochlorobenzene (DNCB) in mice. In this experiment, the effects of CPon changes in body weights, ear and dorsum skin thicknesses, ear weights, clinical aspects on the dorsum skin,histopathological changes, spleen weights, cytokines were investigated. In addition, the effects on the proliferationrates of splenocytes were also investigated in vivo and vitro study. In results, CP spread (CPS) group and CP spread and administered (CPS+Adm) group showed decrease in spleenweights. In CPS+Adm group, dorsum skin thicknesses were decreased significantly compared to control group. CPtreatment diminished erythema, desquamation and keratosis which were induced by repeated painting of DNCB. In histopathological observation, spongiosis and edema were diminished in CPS and CPS+Adm group. CP led todecrease in the proliferation rates of splenocytes in vivo and vitro. In conclusion, these data suggest that CP can decrease symptoms of ACD, so CP is useful to treat patient withACD.
Abstract
Allergic contact dermatitis (ACD) is a type IV delayed hypersensitivity reaction that results from exposures andsubsequent sensitization to an environmental chemical. Crataegus Pinnatifida (CP) is commonly used to improvespleen function, remove retention of food, and promote blood circulation. This study is designed to investigate theeffects of CP on ACD induced by 2,4-dinitrochlorobenzene (DNCB) in mice. In this experiment, the effects of CPon changes in body weights, ear and dorsum skin thicknesses, ear weights, clinical aspects on the dorsum skin,histopathological changes, spleen weights, cytokines were investigated. In addition, the effects on the proliferationrates of splenocytes were also investigated in vivo and vitro study. In results, CP spread (CPS) group and CP spread and administered (CPS+Adm) group showed decrease in spleenweights. In CPS+Adm group, dorsum skin thicknesses were decreased significantly compared to control group. CPtreatment diminished erythema, desquamation and keratosis which were induced by repeated painting of DNCB. In histopathological observation, spongiosis and edema were diminished in CPS and CPS+Adm group. CP led todecrease in the proliferation rates of splenocytes in vivo and vitro. In conclusion, these data suggest that CP can decrease symptoms of ACD, so CP is useful to treat patient withACD.
참고문헌 (42)
- Janeway CA, "Immunobiology", pp.461~ (2005), Lifescience
- 류혜숙, "생강 추출물 투여에 의한 마우스 비장세포 및 대식세포 활성 효과", Journal of Nutrition and Health, 37(9), pp.780~785 (2004), 한국영양학회
- 표명윤, "Mancozeb의 아급성 노출이 마우스의 면역병리학적 인자 및 비장세포 증식능력에 미치는 영향", 환경독성보건학회지, 19(4), pp.367~373 (2004), 환경독성보건학회
- Korean dermatological association, "Dermatology", pp.63~185 (2008), Ryomoongak
- Lee BW, "Bonchojeongyo", pp.346~348 (2002), Iljoongsa
- Hwang KS, "Bonchogujin", pp.202~ (1993), Uiseongdang
- 채종구, "내피세포 Nitric Oxide 유리를 통한 山？의 혈관이완 작용", 동의생리병리학회지, 17(1), pp.145~149 (2003), 대한동의병리학회
- 유가량, "산사 Butanol 분획이 PGF2α-유도 혈관평활근수축의 억제에 미치는 신호전달 연구", 동의생리병리학회지, 17(2), pp.461~466 (2003), 대한동의병리학회
- 최호정, "山？ 분획의 혈관이완 활성과 성분분석에 관한 연구", 동의생리병리학회지, 17(5), pp.1235~1242 (2003), 대한동의병리학회
- 손창우, "山査의 혈관이완 효능과 항산화 작용", 동의생리병리학회지, 16(1), pp.11~71 (2002), 대한동의병리학회
- 권순혁, "山楂가 흰쥐의 식이성 高脂血症에 미치는 영향", 동의생리병리학회지, 24(1), pp.67~73 (2010), 대한동의병리학회
- 서부일, "山楂가 알콜 투여로 유발된 흰쥐의 고지혈증과간 손상의 예방에 미치는 영향", 대한본초학회지, 20(1), pp.35~44 (2005), 대한본초학회
- 신정훈, "카드뮴유발 흰쥐의 간손상에 대한 山査추출물의 보호효과", 동의생리병리학회지, 24(2), pp.249~257 (2010), 대한동의병리학회
- 왕수빈, "기억력 감퇴모델에서 산사의 기억력 개선 효과에 관한 연구", 대한본초학회지, 24(4), pp.165~171 (2009), 대한본초학회
- 임영미, "배양 인체피부섬유모세포에 있어서 활성산소의 산화적 손상에 대한 山査추출물의 항산화 효과", 동의생리병리학회지, 22(1), pp.115~119 (2008), 대한동의병리학회
- Kim KH, "Effect of Crataegii Fructus on antioxidant capacity in D-galactose induced aging rats", Korean J Orient Prev Med Soc, 8(2), pp.65~80 (2004)
- Yoon CY, "Huangdi's internal classics plain questions", pp.531~ (2004), Joomin
- Oriental medicine university dermatology & surgery, "Text of traditional korean dermatology & surgery", pp.65~353 (2007), Sunwoo
- Yuan XY, "Effects and mechanisms of aloperine on 2,4-dinitrofluorobenzeneinduced allergic contact dermatitis in BALB", c mice, 2010, pp.629(1), 147~152
- 김보라, "차가버섯 열수 추출물이 비장세포 증식능과 Cytokine 생성능에 미치는 영향", 약 학 회 지, 54(3), pp.187~191 (2010), 대한약학회
- Ryu JY, "Differentiation of syndromes and treatments in surgery & dermatology", pp.209~211 (1987), Seowandang
- 조백건, "內消和中湯加味方의 아토피 피부염 치료에 대한 임상고찰", 대한한방소아과학회지, 19(1), pp.131~141 (2005), 대한한방소아과학회
- Park EH, "Effects of GP extract on oxidative stress and contact dermatitis in NC", Nga mice induced by DNCB, 2007, pp.16(2), 131~145[DOI]
- 김정환, "脾虛蘊濕方이 NC", Nga 아토피피부염 생쥐 모델의 피부 및 면역학적 변화에 미치는 영향, 2007, pp.21(3), 714~720[DOI]
- Oriental medicine university herbology, "Herbology", pp.415~ (2004), Younglimsa
- Kim Jae Ik., "Clinical herbology lecture", pp.521~ (2000), Daesung
- Shin Tae Yong, "Allergy and oriental medicine", pp.3~147 (2001), Shinil
- Jeong JY, "Common skin disease in korea", pp.37~48 (2009), MD world
- Jeon JH, "A literature study on the external therapy of contact dermatitis", J Korean Orient Med Ophthalmol Otolaryngol Dermatol, 11(1), pp.197~218 (1998)
- 김상찬, "금은화 화장수가 DNCB로 유발된 접촉성피부염에 미치는 영향", 대한본초학회지, 21(1), pp.9~15 (2006), 대한본초학회
- Shin KS, "The effect of Rhemanniae radix extract on allergic contact dermatitis on mice induced by DNCB", Herbal Formula Sci, 8(1), pp.257~279 (2000)
- 송진수, "현삼이 DNCB로 유발된 알레르기성 접촉성 피부염에 미치는 영향", 한방안이비인후피부과학회지, 24(3), pp.1~16 (2011), 대한한방안이비인후피부과학회
- Jun JH, "The effects of Scutellaria baicalensis GEORGI on allergic contact dermatitis induced by DNCB in mice", Dongguk university oriental medicine institute, 7(1), pp.119~133 (1998)
- 강윤호, "黃連解毒湯이 알러지성 접촉피부염에 미치는 영향 - 알러지성 접촉피부염 유발로 손상된 생쥐 상피세포의 완화를 중심으로", 대한한방내과학회지, 25(3), pp.508~517 (2004), 대한한방내과학회
- 박숙자, "소풍산물추출물이 DNCB로 유발된 접촉성피부염에 미치는 영향", 동의생리병리학회지, 20(3), pp.623~628 (2006), 대한동의병리학회
- 남봉수, "散風苦蔘丸이 알레르기성 접촉피부염에 미치는 영향", 한방안이비인후피부과학회지, 18(2), pp.10~27 (2005), 대한한방안이비인후피부과학회
- 배은영, "祛風燥濕解毒方이 접촉성피부염 유발 NC", Nga 생쥐의 면역 관련 인자에 미치는 영향, 2007, pp.21(6), 1491~1498[DOI]
- Kim JK, "Effect of Scrophularia buergeriana extract on the degranulation of mast cells and ear swelling induced by dinitrofluorobenzene in mice", Inflammation, 35(1), pp.183~191 (2012)
- Saint-Mezard P, "Allergic contact dermatitis", Eur J Dermatol, 14(5), pp.284~295 (2004)
- Kim IS, "Core immunology", pp.24~25 (2010), Daihakseolim
- 정인조, "개에서 실험적으로 유발한 비장종대의 방사선학적 평가", 한국임상수의학회지, 23(3), pp.263~266 (2006), 한국임상수의학회
- Kwon HH, "Clinical immunology" (2010), Koreamedicalbook